EXPRESSION OF A BIOLOGICALLY-ACTIVE MURINE TISSUE INHIBITOR OF METALLOPROTEINASES-1 (TIMP-1) IN BACULOVIRUS-INFECTED INSECT CELLS - PURIFICATION AND TISSUE DISTRIBUTION IN THE RAT

KURSCHAT P, GRAEVE L, ERREN A, GATSIOS P, ROSEJOHN S, ROEB E, Tschesche H, KOJ A, HEINRICH PC (1995)
EUROPEAN JOURNAL OF BIOCHEMISTRY 234(2): 485-491.

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Murine tissue inhibitor of metalloproteinases-1 (mTIMP-1) was expressed in baculovirus-infected insect cells (Sf9). The protein secreted into the culture medium was purified to homogeneity by means of heparin-Sepharose CL-6B and FPLC. The purified protein showed metalloproteinase-inhibitory activity in two independent assays: reverse zymography and inhibition of collagenase activity. Digestion of the recombinant TIMP-1 with peptide-N-glycanaseF revealed that both N-glycosylation sites are used. I-125-mTIMP-1 intraveneously injected into a male Sprague Dawley rat disappeared within 2 min from the circulation. 5 min after injection more than 50% of the I-125-mTIMP-1 were found in the liver and 20% in the kidneys. At later times, trichloroacetic-acid-soluble material accumulated in the intestinal tract.
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KURSCHAT P, GRAEVE L, ERREN A, et al. EXPRESSION OF A BIOLOGICALLY-ACTIVE MURINE TISSUE INHIBITOR OF METALLOPROTEINASES-1 (TIMP-1) IN BACULOVIRUS-INFECTED INSECT CELLS - PURIFICATION AND TISSUE DISTRIBUTION IN THE RAT. EUROPEAN JOURNAL OF BIOCHEMISTRY. 1995;234(2):485-491.
KURSCHAT, P., GRAEVE, L., ERREN, A., GATSIOS, P., ROSEJOHN, S., ROEB, E., Tschesche, H., et al. (1995). EXPRESSION OF A BIOLOGICALLY-ACTIVE MURINE TISSUE INHIBITOR OF METALLOPROTEINASES-1 (TIMP-1) IN BACULOVIRUS-INFECTED INSECT CELLS - PURIFICATION AND TISSUE DISTRIBUTION IN THE RAT. EUROPEAN JOURNAL OF BIOCHEMISTRY, 234(2), 485-491.
KURSCHAT, P., GRAEVE, L., ERREN, A., GATSIOS, P., ROSEJOHN, S., ROEB, E., Tschesche, H., KOJ, A., and HEINRICH, P. C. (1995). EXPRESSION OF A BIOLOGICALLY-ACTIVE MURINE TISSUE INHIBITOR OF METALLOPROTEINASES-1 (TIMP-1) IN BACULOVIRUS-INFECTED INSECT CELLS - PURIFICATION AND TISSUE DISTRIBUTION IN THE RAT. EUROPEAN JOURNAL OF BIOCHEMISTRY 234, 485-491.
KURSCHAT, P., et al., 1995. EXPRESSION OF A BIOLOGICALLY-ACTIVE MURINE TISSUE INHIBITOR OF METALLOPROTEINASES-1 (TIMP-1) IN BACULOVIRUS-INFECTED INSECT CELLS - PURIFICATION AND TISSUE DISTRIBUTION IN THE RAT. EUROPEAN JOURNAL OF BIOCHEMISTRY, 234(2), p 485-491.
P. KURSCHAT, et al., “EXPRESSION OF A BIOLOGICALLY-ACTIVE MURINE TISSUE INHIBITOR OF METALLOPROTEINASES-1 (TIMP-1) IN BACULOVIRUS-INFECTED INSECT CELLS - PURIFICATION AND TISSUE DISTRIBUTION IN THE RAT”, EUROPEAN JOURNAL OF BIOCHEMISTRY, vol. 234, 1995, pp. 485-491.
KURSCHAT, P., GRAEVE, L., ERREN, A., GATSIOS, P., ROSEJOHN, S., ROEB, E., Tschesche, H., KOJ, A., HEINRICH, P.C.: EXPRESSION OF A BIOLOGICALLY-ACTIVE MURINE TISSUE INHIBITOR OF METALLOPROTEINASES-1 (TIMP-1) IN BACULOVIRUS-INFECTED INSECT CELLS - PURIFICATION AND TISSUE DISTRIBUTION IN THE RAT. EUROPEAN JOURNAL OF BIOCHEMISTRY. 234, 485-491 (1995).
KURSCHAT, P, GRAEVE, L, ERREN, A, GATSIOS, P, ROSEJOHN, S, ROEB, E, Tschesche, Harald, KOJ, A, and HEINRICH, PC. “EXPRESSION OF A BIOLOGICALLY-ACTIVE MURINE TISSUE INHIBITOR OF METALLOPROTEINASES-1 (TIMP-1) IN BACULOVIRUS-INFECTED INSECT CELLS - PURIFICATION AND TISSUE DISTRIBUTION IN THE RAT”. EUROPEAN JOURNAL OF BIOCHEMISTRY 234.2 (1995): 485-491.
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5 Citations in Europe PMC

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Batra J, Robinson J, Mehner C, Hockla A, Miller E, Radisky DC, Radisky ES., PLoS ONE 7(11), 2012
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Brain distribution and elimination of recombinant human TIMP-1 after cerebral ischemia and reperfusion in rats.
Sa Y, Hao J, Samineni D, Clark J, Pyne-Geithman G, Broderick J, Lu A., Neurol. Res. 33(4), 2011
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TIMP-1 inhibits apoptosis in breast carcinoma cells via a pathway involving pertussis toxin-sensitive G protein and c-Src.
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Gaudin P, Trocme C, Berthier S, Kieffer S, Boutonnat J, Lamy C, Surla A, Garin J, Morel F., Biochim. Biophys. Acta 1499(1-2), 2000
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Oncostatin M differentially regulates tissue inhibitors of metalloproteinases TIMP-1 and TIMP-3 gene expression in human synovial lining cells.
Gatsios P, Haubeck HD, Van de Leur E, Frisch W, Apte SS, Greiling H, Heinrich PC, Graeve L., Eur. J. Biochem. 241(1), 1996
PMID: 8898888

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