The promoter of the Vicia faba L VfENOD-GRP3 gene encoding a glycine-rich early nodulin mediates a predominant gene expression in the interzone II-III region of transgenic Vicia hirsuta root nodules

Küster H, Quandt HJ, Broer I, Perlick AM, Pühler A (1995)
PLANT MOLECULAR BIOLOGY 29(4): 759-772.

Download
Es wurde kein Volltext hochgeladen. Nur Publikationsnachweis!
Zeitschriftenaufsatz | Veröffentlicht | Englisch
Autor
; ; ; ;
Abstract / Bemerkung
We recently reported on the broad bean gene VfENOD-GRP3 encoding a glycine-rich early nodulin. This gene was predominantly expressed in the interzone II-III region of Vicia faba root nodules. The VfENOD-GRP3 promoter contained several sequence motifs potentially involved in the regulation of gene expression. To investigate the molecular basis for the specific VfENOD-GRP3 expression, defined VfENOD-GRP3 promoter fragments were fused to an intron-containing gusAint gene. Agrobacterium rhizogenes ARqual strains carrying these fusions integrated into the TL DNA were used to generate hairy roots on Vicia hirsuta, which subsequently were nodulated. Histochemical analysis of transgenic nodules indicated that a strong gusAint expression in the interzone II-III region was mediated by the -1252/+10 VfENOD-GRP3 promoter region. This reporter gene expression in V. hirsuta was comparable to the location of VfENOD-GRP3 transcripts in V. faba nodules. An analysis of defined promoter fragments revealed that a strong gusAint expression in the interzone II-III region was also mediated by the -737/+10 promoter, whereas the -239/+10 promoter only mediated a weak gusAint expression in the interzone II-III region. Since the -239/+10 promoter fragment did not resemble published nodulin gene promoters, we propose that it contains new sequence motifs involved in mediating gene expression in the interzone II-III region of Vicia nodules.
Erscheinungsjahr
Zeitschriftentitel
PLANT MOLECULAR BIOLOGY
Band
29
Zeitschriftennummer
4
Seite
759-772
ISSN
eISSN
PUB-ID

Zitieren

Küster H, Quandt HJ, Broer I, Perlick AM, Pühler A. The promoter of the Vicia faba L VfENOD-GRP3 gene encoding a glycine-rich early nodulin mediates a predominant gene expression in the interzone II-III region of transgenic Vicia hirsuta root nodules. PLANT MOLECULAR BIOLOGY. 1995;29(4):759-772.
Küster, H., Quandt, H. J., Broer, I., Perlick, A. M., & Pühler, A. (1995). The promoter of the Vicia faba L VfENOD-GRP3 gene encoding a glycine-rich early nodulin mediates a predominant gene expression in the interzone II-III region of transgenic Vicia hirsuta root nodules. PLANT MOLECULAR BIOLOGY, 29(4), 759-772. doi:10.1007/BF00041166
Küster, H., Quandt, H. J., Broer, I., Perlick, A. M., and Pühler, A. (1995). The promoter of the Vicia faba L VfENOD-GRP3 gene encoding a glycine-rich early nodulin mediates a predominant gene expression in the interzone II-III region of transgenic Vicia hirsuta root nodules. PLANT MOLECULAR BIOLOGY 29, 759-772.
Küster, H., et al., 1995. The promoter of the Vicia faba L VfENOD-GRP3 gene encoding a glycine-rich early nodulin mediates a predominant gene expression in the interzone II-III region of transgenic Vicia hirsuta root nodules. PLANT MOLECULAR BIOLOGY, 29(4), p 759-772.
H. Küster, et al., “The promoter of the Vicia faba L VfENOD-GRP3 gene encoding a glycine-rich early nodulin mediates a predominant gene expression in the interzone II-III region of transgenic Vicia hirsuta root nodules”, PLANT MOLECULAR BIOLOGY, vol. 29, 1995, pp. 759-772.
Küster, H., Quandt, H.J., Broer, I., Perlick, A.M., Pühler, A.: The promoter of the Vicia faba L VfENOD-GRP3 gene encoding a glycine-rich early nodulin mediates a predominant gene expression in the interzone II-III region of transgenic Vicia hirsuta root nodules. PLANT MOLECULAR BIOLOGY. 29, 759-772 (1995).
Küster, Helge, Quandt, HJ, Broer, I, Perlick, AM, and Pühler, Alfred. “The promoter of the Vicia faba L VfENOD-GRP3 gene encoding a glycine-rich early nodulin mediates a predominant gene expression in the interzone II-III region of transgenic Vicia hirsuta root nodules”. PLANT MOLECULAR BIOLOGY 29.4 (1995): 759-772.

9 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Composite potato plants with transgenic roots on non-transgenic shoots: a model system for studying gene silencing in roots.
Horn P, Santala J, Nielsen SL, Hühns M, Broer I, Valkonen JP., Plant Cell Rep 33(12), 2014
PMID: 25182479
The signal peptide of the Medicago truncatula modular nodulin MtNOD25 operates as an address label for the specific targeting of proteins to nitrogen-fixing symbiosomes.
Hohnjec N, Lenz F, Fehlberg V, Vieweg MF, Baier MC, Hause B, Küster H., Mol Plant Microbe Interact 22(1), 2009
PMID: 19061403
Glycine-rich proteins encoded by a nodule-specific gene family are implicated in different stages of symbiotic nodule development in Medicago spp.
Kevei Z, Vinardell JM, Kiss GB, Kondorosi A, Kondorosi E., Mol Plant Microbe Interact 15(9), 2002
PMID: 12236598

51 References

Daten bereitgestellt von Europe PubMed Central.


J, Nucl Acids Res 18(), 1990

Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®

Quellen

PMID: 8541502
PubMed | Europe PMC

Suchen in

Google Scholar