The promoter of the Vicia faba L VfENOD-GRP3 gene encoding a glycine-rich early nodulin mediates a predominant gene expression in the interzone II-III region of transgenic Vicia hirsuta root nodules

Küster H, Quandt HJ, Broer I, Perlick AM, Pühler A (1995)
PLANT MOLECULAR BIOLOGY 29(4): 759-772.

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Zeitschriftenaufsatz | Veröffentlicht | Englisch
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Abstract / Bemerkung
We recently reported on the broad bean gene VfENOD-GRP3 encoding a glycine-rich early nodulin. This gene was predominantly expressed in the interzone II-III region of Vicia faba root nodules. The VfENOD-GRP3 promoter contained several sequence motifs potentially involved in the regulation of gene expression. To investigate the molecular basis for the specific VfENOD-GRP3 expression, defined VfENOD-GRP3 promoter fragments were fused to an intron-containing gusAint gene. Agrobacterium rhizogenes ARqual strains carrying these fusions integrated into the TL DNA were used to generate hairy roots on Vicia hirsuta, which subsequently were nodulated. Histochemical analysis of transgenic nodules indicated that a strong gusAint expression in the interzone II-III region was mediated by the -1252/+10 VfENOD-GRP3 promoter region. This reporter gene expression in V. hirsuta was comparable to the location of VfENOD-GRP3 transcripts in V. faba nodules. An analysis of defined promoter fragments revealed that a strong gusAint expression in the interzone II-III region was also mediated by the -737/+10 promoter, whereas the -239/+10 promoter only mediated a weak gusAint expression in the interzone II-III region. Since the -239/+10 promoter fragment did not resemble published nodulin gene promoters, we propose that it contains new sequence motifs involved in mediating gene expression in the interzone II-III region of Vicia nodules.
Erscheinungsjahr
Zeitschriftentitel
PLANT MOLECULAR BIOLOGY
Band
29
Ausgabe
4
Seite(n)
759-772
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Küster H, Quandt HJ, Broer I, Perlick AM, Pühler A. The promoter of the Vicia faba L VfENOD-GRP3 gene encoding a glycine-rich early nodulin mediates a predominant gene expression in the interzone II-III region of transgenic Vicia hirsuta root nodules. PLANT MOLECULAR BIOLOGY. 1995;29(4):759-772.
Küster, H., Quandt, H. J., Broer, I., Perlick, A. M., & Pühler, A. (1995). The promoter of the Vicia faba L VfENOD-GRP3 gene encoding a glycine-rich early nodulin mediates a predominant gene expression in the interzone II-III region of transgenic Vicia hirsuta root nodules. PLANT MOLECULAR BIOLOGY, 29(4), 759-772. doi:10.1007/BF00041166
Küster, H., Quandt, H. J., Broer, I., Perlick, A. M., and Pühler, A. (1995). The promoter of the Vicia faba L VfENOD-GRP3 gene encoding a glycine-rich early nodulin mediates a predominant gene expression in the interzone II-III region of transgenic Vicia hirsuta root nodules. PLANT MOLECULAR BIOLOGY 29, 759-772.
Küster, H., et al., 1995. The promoter of the Vicia faba L VfENOD-GRP3 gene encoding a glycine-rich early nodulin mediates a predominant gene expression in the interzone II-III region of transgenic Vicia hirsuta root nodules. PLANT MOLECULAR BIOLOGY, 29(4), p 759-772.
H. Küster, et al., “The promoter of the Vicia faba L VfENOD-GRP3 gene encoding a glycine-rich early nodulin mediates a predominant gene expression in the interzone II-III region of transgenic Vicia hirsuta root nodules”, PLANT MOLECULAR BIOLOGY, vol. 29, 1995, pp. 759-772.
Küster, H., Quandt, H.J., Broer, I., Perlick, A.M., Pühler, A.: The promoter of the Vicia faba L VfENOD-GRP3 gene encoding a glycine-rich early nodulin mediates a predominant gene expression in the interzone II-III region of transgenic Vicia hirsuta root nodules. PLANT MOLECULAR BIOLOGY. 29, 759-772 (1995).
Küster, Helge, Quandt, HJ, Broer, I, Perlick, AM, and Pühler, Alfred. “The promoter of the Vicia faba L VfENOD-GRP3 gene encoding a glycine-rich early nodulin mediates a predominant gene expression in the interzone II-III region of transgenic Vicia hirsuta root nodules”. PLANT MOLECULAR BIOLOGY 29.4 (1995): 759-772.

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51 References

Daten bereitgestellt von Europe PubMed Central.


W, Gene 70(), 1998

T, Plant Mol Biol Rep 12(), 1994
Development of the legume root nodule.
Brewin NJ., Annu. Rev. Cell Biol. 7(), 1991
PMID: 1809347

WC, Bio Techniques 5(), 1987
Plant genetic control of nodulation.
Caetano-Anolles G, Gresshoff PM., Annu. Rev. Microbiol. 45(), 1991
PMID: 1741618

FJ, 1992

FJ, Plant Soil 161(), 1994
Signalling strategies for nodulation of legumes by rhizobia.
Downie JA., Trends Microbiol. 2(9), 1994
PMID: 7812664
Developmental aspects of the Rhizobium-legume symbiosis.
Franssen HJ, Vijn I, Yang WC, Bisseling T., Plant Mol. Biol. 19(1), 1992
PMID: 1600171

A, New Phytol 122(), 1992

RA, Plant Mol Biol Rep 5(), 1987
A two-component nodule-specific enhancer in the soybean N23 gene promoter.
Jorgensen JE, Stougaard J, Marcker KA., Plant Cell 3(8), 1991
PMID: 1820821

E-P, Plant J 6(), 1993

C, 1992

H, Mol Plant-Microbe Interact 6(), 1993
Conserved regulation of the soybean early nodulin ENOD2 gene promoter in determine and indeterminate transgenic root nodules.
Lauridsen P, Franssen H, Stougaard J, Bisseling T, Marcker KA., Plant J. 3(3), 1993
PMID: 8220455

D, Nucl Acids Res 17(), 1989

HM, J Bact 149(), 1982

JP, Physiol Plant 79(), 1990

AM, 1994
Rhizobium meliloti elicits transient expression of the early nodulin gene ENOD12 in the differentiating root epidermis of transgenic alfalfa.
Pichon M, Journet EP, Dedieu A, de Billy F, Truchet G, Barker DG., Plant Cell 4(10), 1992
PMID: 1446169

M, Mol Plant-Microbe Interact 7(), 1994

H-J, Mol Plant-Microbe Interact 6(), 1993

H-J, 1994
Site-directed mutagenesis of the organ-specific element in the soybean leghemoglobin lbc3 gene promoter.
Ramlov KB, Laursen NB, Stougaard J, Marcker KA., Plant J. 4(3), 1993
PMID: 8220496

J, 1989
Sequential induction of nodulin gene expression in the developing pea nodule.
Scheres B, van Engelen F, van der Knaap E, van de Wiel C, van Kammen A, Bisseling T., Plant Cell 2(8), 1990
PMID: 2152123
Comparative sequence analysis of cis elements present in Glycine max L. leghemoglobin lba and lbc3 genes.
She Q, Sandal NN, Stougaard J, Marcker KA., Plant Mol. Biol. 22(5), 1993
PMID: 8358040

R, Bio technology 1(), 1983

J, Mol Gen Genet 207(), 1987

A, Plant Mol Biol Rep 2(), 1984

J, J Bact 172(), 1990

DPS, Physiol Plant 85(), 1992
Characterization of different plaque-forming and defective temperate phages in Agrobacterium.
Vervliet G, Holsters M, Teuchy H, Van Montagu M, Schell J., J. Gen. Virol. 26(1), 1975
PMID: 1123610

J, Nucl Acids Res 18(), 1990

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