The broad bean gene VfNOD32 encodes a nodulin with sequence similarities to chitinases that is homologous to (alpha/beta)(8)-barrel-type seed proteins

Perlick AM, Fruhling M, Schroder G, Frosch SC, Pühler A (1996)
PLANT PHYSIOLOGY 110(1): 147-154.

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Abstract
Nodulin gene transcripts isolated from a broad bean (Vicia faba L.) root nodule cDNA library and designated VfNOD32 are detectable in the nitrogen-fixing zone III of nodules and in much smaller amounts in flowers. In nodules, these transcripts are detectable for the first time 7 d after inoculation, at least 1 d before leghemoglobin gene transcription starts. Two putative full-length cDNAs representing different transcript sequences of 92.5% identity were sequenced. The corresponding broad bean genes were termed VfNOD32-A1 and VfNOD32-A2, and the encoded proteins were termed Nvf32-A1 and Nvf32-A2. The derived amino acid sequences of the Nvf32 proteins are highly homologous to the Vicia narbonensis (alpha/beta)(8)-barrel seed protein narbonin. Considering this homology, Nvf32 is assumed to have a similar structure consisting of beta-sheets forming a central barrel surrounded by alpha-helices. The two Nvf32 sequences also contain two conserved amino acid motifs that are characteristic of class-III chitinases. Several amino acids demonstrated to be essential for chitinase activity are conserved in both regions, whereas one essential glutamic acid was changed to glycine in the Nvf32-A1 isoform but not in the Nvf32-A2 isoform.
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Perlick AM, Fruhling M, Schroder G, Frosch SC, Pühler A. The broad bean gene VfNOD32 encodes a nodulin with sequence similarities to chitinases that is homologous to (alpha/beta)(8)-barrel-type seed proteins. PLANT PHYSIOLOGY. 1996;110(1):147-154.
Perlick, A. M., Fruhling, M., Schroder, G., Frosch, S. C., & Pühler, A. (1996). The broad bean gene VfNOD32 encodes a nodulin with sequence similarities to chitinases that is homologous to (alpha/beta)(8)-barrel-type seed proteins. PLANT PHYSIOLOGY, 110(1), 147-154.
Perlick, A. M., Fruhling, M., Schroder, G., Frosch, S. C., and Pühler, A. (1996). The broad bean gene VfNOD32 encodes a nodulin with sequence similarities to chitinases that is homologous to (alpha/beta)(8)-barrel-type seed proteins. PLANT PHYSIOLOGY 110, 147-154.
Perlick, A.M., et al., 1996. The broad bean gene VfNOD32 encodes a nodulin with sequence similarities to chitinases that is homologous to (alpha/beta)(8)-barrel-type seed proteins. PLANT PHYSIOLOGY, 110(1), p 147-154.
A.M. Perlick, et al., “The broad bean gene VfNOD32 encodes a nodulin with sequence similarities to chitinases that is homologous to (alpha/beta)(8)-barrel-type seed proteins”, PLANT PHYSIOLOGY, vol. 110, 1996, pp. 147-154.
Perlick, A.M., Fruhling, M., Schroder, G., Frosch, S.C., Pühler, A.: The broad bean gene VfNOD32 encodes a nodulin with sequence similarities to chitinases that is homologous to (alpha/beta)(8)-barrel-type seed proteins. PLANT PHYSIOLOGY. 110, 147-154 (1996).
Perlick, AM, Fruhling, M, Schroder, G, Frosch, SC, and Pühler, Alfred. “The broad bean gene VfNOD32 encodes a nodulin with sequence similarities to chitinases that is homologous to (alpha/beta)(8)-barrel-type seed proteins”. PLANT PHYSIOLOGY 110.1 (1996): 147-154.
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