Matrix metalloproteinases and their inhibitors in gingival crevicular fluid and saliva of periodontitis patients

Ingman T, Tervahartiala T, Ding YL, Tschesche H, Haerian A, Kinane DF, Konttinen YT, Sorsa T (1996)
JOURNAL OF CLINICAL PERIODONTOLOGY 23(12): 1127-1132.

Download
No fulltext has been uploaded. References only!
Journal Article | Original Article | Published | English

No fulltext has been uploaded

Author
; ; ; ; ; ; ;
Abstract
Matrix metalloproteinases (MMPs) and serine proteinases seem to be related to tissue destruction in periodontitis. The presence of MMPs in gingival crevicular fluid (GCF) and saliva, however, has not been studied comprehensively with the enzyme-linked immunosorbent assay (ELISA)-technique, We therefore examined the levels of MMP-1, -3, -8 and -9, and their endogenous inhibitor, tissue inhibitor of matrix metalloproteinases (TIMP-1), in GCF and saliva of patients with adult periodontitis (AP) and localized juvenile periodontitis (LJP). Elevated levels of MMP-1 were detected in LJP GCF compared to AP and control GCE Elevated levels of TIMP-1 were also detected in LJP GCF in comparison to AP and control GCE Higher MMP-8 levels were detected in AP GCF compared to LJP and control GCE The relative low levels of MMP-3 were present in all studied GCF samples. Elevated levels of MMP-8 were further detected in saliva of AP compared to LJP and the controls. Both MMP-1 and TIMP-1 were detected in all studied saliva samples, but not significant differences were detected between the studied groups. Our ELISA-results confirm that (i) PMN MMP-8 and MMP-9 are the main collagenase and gelatinase in AP GCF, whereas GCF collagenase in LJP seems to be of the MMP-1-type; (ii) only low levels of TIMP-1, endogenous MMP-inhibitor, are present in AP GCE which emphasises the importance of doxycycline as a possible adjunctive drug in the treatment of AP patients; (iii) tests based on specific antibodies against PMN MMPs, especially MMP-8, might serve as a reliable method of measuring and monitoring enzyme levels in GCF from different periodontitis patients.
Publishing Year
ISSN
eISSN
PUB-ID

Cite this

Ingman T, Tervahartiala T, Ding YL, et al. Matrix metalloproteinases and their inhibitors in gingival crevicular fluid and saliva of periodontitis patients. JOURNAL OF CLINICAL PERIODONTOLOGY. 1996;23(12):1127-1132.
Ingman, T., Tervahartiala, T., Ding, Y. L., Tschesche, H., Haerian, A., Kinane, D. F., Konttinen, Y. T., et al. (1996). Matrix metalloproteinases and their inhibitors in gingival crevicular fluid and saliva of periodontitis patients. JOURNAL OF CLINICAL PERIODONTOLOGY, 23(12), 1127-1132. doi:10.1111/j.1600-051X.1996.tb01814.x
Ingman, T., Tervahartiala, T., Ding, Y. L., Tschesche, H., Haerian, A., Kinane, D. F., Konttinen, Y. T., and Sorsa, T. (1996). Matrix metalloproteinases and their inhibitors in gingival crevicular fluid and saliva of periodontitis patients. JOURNAL OF CLINICAL PERIODONTOLOGY 23, 1127-1132.
Ingman, T., et al., 1996. Matrix metalloproteinases and their inhibitors in gingival crevicular fluid and saliva of periodontitis patients. JOURNAL OF CLINICAL PERIODONTOLOGY, 23(12), p 1127-1132.
T. Ingman, et al., “Matrix metalloproteinases and their inhibitors in gingival crevicular fluid and saliva of periodontitis patients”, JOURNAL OF CLINICAL PERIODONTOLOGY, vol. 23, 1996, pp. 1127-1132.
Ingman, T., Tervahartiala, T., Ding, Y.L., Tschesche, H., Haerian, A., Kinane, D.F., Konttinen, Y.T., Sorsa, T.: Matrix metalloproteinases and their inhibitors in gingival crevicular fluid and saliva of periodontitis patients. JOURNAL OF CLINICAL PERIODONTOLOGY. 23, 1127-1132 (1996).
Ingman, T, Tervahartiala, T, Ding, YL, Tschesche, Harald, Haerian, A, Kinane, DF, Konttinen, YT, and Sorsa, T. “Matrix metalloproteinases and their inhibitors in gingival crevicular fluid and saliva of periodontitis patients”. JOURNAL OF CLINICAL PERIODONTOLOGY 23.12 (1996): 1127-1132.
This data publication is cited in the following publications:
This publication cites the following data publications:

166 Citations in Europe PMC

Data provided by Europe PubMed Central.

Inhibition of human gingival gelatinases (MMP-2 and MMP-9) by metal salts.
de Souza AP, Gerlach RF, Line SR., Dent Mater 16(2), 2000
PMID: 11203530
Use of a fluorogenic septapeptide matrix metalloproteinase assay to assess responses to periodontal treatment.
Bhide VM, Smith L, Overall CM, Birek P, McCulloch CA., J Periodontol 71(5), 2000
PMID: 10872948
Inhibition of the activities of matrix metalloproteinases 2, 8, and 9 by chlorhexidine.
Gendron R, Grenier D, Sorsa T, Mayrand D., Clin Diagn Lab Immunol 6(3), 1999
PMID: 10225852
Scientific basis of a matrix metalloproteinase-8 specific chair-side test for monitoring periodontal and peri-implant health and disease.
Sorsa T, Mäntylä P, Rönkä H, Kallio P, Kallis GB, Lundqvist C, Kinane DF, Salo T, Golub LM, Teronen O, Tikanoja S., Ann N Y Acad Sci 878(), 1999
PMID: 10415725
Assessment of a novel screening test for neutrophil collagenase activity in the diagnosis of periodontal diseases.
Mancini S, Romanelli R, Laschinger CA, Overall CM, Sodek J, McCulloch CA., J Periodontol 70(11), 1999
PMID: 10588492
The association between smoking cessation and periodontal status and salivary proteinase levels.
Liede KE, Haukka JK, Hietanen JH, Mattila MH, Rönkä H, Sorsa T., J Periodontol 70(11), 1999
PMID: 10588500
Dissolution of type I collagen fibrils by gingival fibroblasts isolated from patients of various periodontitis categories.
Havemose-Poulsen A, Holmstrup P, Stoltze K, Birkedal-Hansen H., J Periodontal Res 33(5), 1998
PMID: 9777595
The activation and function of host matrix metalloproteinases in dentin matrix breakdown in caries lesions.
Tjäderhane L, Larjava H, Sorsa T, Uitto VJ, Larmas M, Salo T., J Dent Res 77(8), 1998
PMID: 9719036
Human neutrophil collagenase MMP-8 in peri-implant sulcus fluid and its inhibition by clodronate.
Teronen O, Konttinen YT, Lindqvist C, Salo T, Ingman T, Lauhio A, Ding Y, Santavirta S, Sorsa T., J Dent Res 76(9), 1997
PMID: 9294486
Human neutrophil gelatinase and associated lipocalin in adult and localized juvenile periodontitis.
Westerlund U, Ingman T, Lukinmaa PL, Salo T, Kjeldsen L, Borregaard N, Tjäderhane L, Konttinen YT, Sorsa T., J Dent Res 75(8), 1996
PMID: 8906123

43 References

Data provided by Europe PubMed Central.

Cathepsin G in gingival tissue and crevicular fluid in adult periodontitis.
Tervahartiala T, Konttinen YT, INgman T, Hayrinen-Immonen R, Ding Y, Sorsa T., J. Clin. Periodontol. 23(2), 1996
PMID: 8849841
Cellular source, activation and inhibition of dental plaque collagenase.
Sorsa T, Ding YL, Ingman T, Salo T, Westerlund U, Haapasalo M, Tschesche H, Konttinen YT., J. Clin. Periodontol. 22(9), 1995
PMID: 7593702
Multiple forms of gelatinases/type IV collagenases in saliva and gingival crevicular fluid of periodontitis patients.
Ingman T, Sorsa T, Lindy O, Koski H, Konttinen YT., J. Clin. Periodontol. 21(1), 1994
PMID: 8126240
Lactoferrin in the gingival crevice as a marker of polymorphonuclear leucocytes in periodontal diseases.
Adonogianaki E, Moughal NA, Kinane DF., J. Clin. Periodontol. 20(1), 1993
PMID: 8421112
Comparison of interstitial collagenases from human gingiva, sulcular fluid and polymorphonuclear leukocytes.
Sorsa T, Uitto VJ, Suomalainen K, Vauhkonen M, Lindy S., J. Periodont. Res. 23(6), 1988
PMID: 2851042
Salivary collagenase. Origin, characteristics and relationship to periodontal health.
Uitto VJ, Suomalainen K, Sorsa T., J. Periodont. Res. 25(3), 1990
PMID: 2163444
Immunolocalization of matrix metalloproteinases and TIMP-1 (tissue inhibitor of metalloproteinases) in human gingival tissues from periodontitis patients.
Meikle MC, Hembry RM, Holley J, Horton C, McFarlane CG, Reynolds JJ., J. Periodont. Res. 29(2), 1994
PMID: 8158500
Detection of interleukin-8 and matrix metalloproteinases transcripts in healthy and diseased gingival biopsies by RNA/PCR.
Tonetti MS, Freiburghaus K, Lang NP, Bickel M., J. Periodont. Res. 28(6 Pt 2), 1993
PMID: 8263721
Effects of tetracyclines on neutrophil, gingival, and salivary collagenases. A functional and western-blot assessment with special reference to their cellular sources in periodontal diseases.
Sorsa T, Ding Y, Salo T, Lauhio A, Teronen O, Ingman T, Ohtani H, Andoh N, Takeha S, Konttinen YT., Ann. N. Y. Acad. Sci. 732(), 1994
PMID: 7978785
Collagenase activity in gingival crevicular fluid of patients with juvenile periodontitis.
Suomalainen K, Sorsa T, Saxen L, Vauhkonen M, Uitto VJ., Oral Microbiol. Immunol. 6(1), 1991
PMID: 1658711
Tetracycline inhibition identifies the cellular origin of interstitial collagenases in human periodontal diseases in vivo.
Suomalainen K, Sorsa T, Ingman T, Lindy O, Golub LM., Oral Microbiol. Immunol. 7(2), 1992
PMID: 1326740
Salivary collagenase, elastase- and trypsin-like proteases as biochemical markers of periodontal tissue destruction in adult and localized juvenile periodontitis.
Ingman T, Sorsa T, Konttinen YT, Liede K, Saari H, Lindy O, Suomalainen K., Oral Microbiol. Immunol. 8(5), 1993
PMID: 8265204

Export

0 Marked Publications

Open Data PUB

Web of Science

View record in Web of Science®

Sources

PMID: 8997658
PubMed | Europe PMC

Search this title in

Google Scholar