Ca-dependent regulation of Na+-selective channels via actin cytoskeleton modification in leukemia cells

Maximov AV, Vedernikova EA, Hinssen H, Khaitlina SY, Negulyaev YA (1997)
FEBS LETTERS 412(1): 94-96.

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Abstract
With the use of the patch-clamp technique, physiological mechanisms of Na+ channel regulation involving submembranous actin rearrangements were examined in human myeloid leukemia K562 cells. We found that the actin-severing protein gelsolin applied to cytoplasmic surface of membrane fragments at a high level of [Ca2+](i) (1 mu M) increased drastically the activity of Na-selective channels of 12 pS unitary conductance. In the experiments on intact cells, the elevation of [Ca2+](i) using the ionophore 4Br-A23187 also resulted in Na+ channel activation. Addition of actin to the cytoplasmic surface of membrane patches reduced this activity to background level, likely due to actin polymerization. Our data imply that Ca-dependent modulations of the actin cytoskeleton may represent one of the general mechanisms of channel regulation and cell signalling. (C) 1997 Federation of European Biochemical Societies.
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Maximov AV, Vedernikova EA, Hinssen H, Khaitlina SY, Negulyaev YA. Ca-dependent regulation of Na+-selective channels via actin cytoskeleton modification in leukemia cells. FEBS LETTERS. 1997;412(1):94-96.
Maximov, A. V., Vedernikova, E. A., Hinssen, H., Khaitlina, S. Y., & Negulyaev, Y. A. (1997). Ca-dependent regulation of Na+-selective channels via actin cytoskeleton modification in leukemia cells. FEBS LETTERS, 412(1), 94-96.
Maximov, A. V., Vedernikova, E. A., Hinssen, H., Khaitlina, S. Y., and Negulyaev, Y. A. (1997). Ca-dependent regulation of Na+-selective channels via actin cytoskeleton modification in leukemia cells. FEBS LETTERS 412, 94-96.
Maximov, A.V., et al., 1997. Ca-dependent regulation of Na+-selective channels via actin cytoskeleton modification in leukemia cells. FEBS LETTERS, 412(1), p 94-96.
A.V. Maximov, et al., “Ca-dependent regulation of Na+-selective channels via actin cytoskeleton modification in leukemia cells”, FEBS LETTERS, vol. 412, 1997, pp. 94-96.
Maximov, A.V., Vedernikova, E.A., Hinssen, H., Khaitlina, S.Y., Negulyaev, Y.A.: Ca-dependent regulation of Na+-selective channels via actin cytoskeleton modification in leukemia cells. FEBS LETTERS. 412, 94-96 (1997).
Maximov, AV, Vedernikova, EA, Hinssen, Horst, Khaitlina, SY, and Negulyaev, YA. “Ca-dependent regulation of Na+-selective channels via actin cytoskeleton modification in leukemia cells”. FEBS LETTERS 412.1 (1997): 94-96.
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