The cytoplasmic DNA-binding protein TraM binds to the inner membrane protein TraD in vitro

DisqueKochem C, Dreiseikelmann B (1997)
JOURNAL OF BACTERIOLOGY 179(19): 6133-6137.

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Zeitschriftenaufsatz | Veröffentlicht | Englisch
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Abstract / Bemerkung
The cytoplasmic protein TraM is one of four essential gene products of the F factor which are involved in DNA transfer after mating pair formation. TraM binds to three specific sites within the oriT region. Besides regulation of its own synthesis, the precise function of TraM during conjugation is not yet known. In the present work, the affinity of TraM to TraD was studied in vitro by an overlay assay and by affinity chromatography. Whether the interaction between TraM and TraD causes a transient or permanent anchoring of the F factor to the site of transfer is discussed, A 35-kDa host membrane protein of yet unknown function also shows affinity to TraM and may be involved in this anchoring process as well.
Erscheinungsjahr
Zeitschriftentitel
JOURNAL OF BACTERIOLOGY
Band
179
Zeitschriftennummer
19
Seite
6133-6137
ISSN
PUB-ID

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DisqueKochem C, Dreiseikelmann B. The cytoplasmic DNA-binding protein TraM binds to the inner membrane protein TraD in vitro. JOURNAL OF BACTERIOLOGY. 1997;179(19):6133-6137.
DisqueKochem, C., & Dreiseikelmann, B. (1997). The cytoplasmic DNA-binding protein TraM binds to the inner membrane protein TraD in vitro. JOURNAL OF BACTERIOLOGY, 179(19), 6133-6137.
DisqueKochem, C., and Dreiseikelmann, B. (1997). The cytoplasmic DNA-binding protein TraM binds to the inner membrane protein TraD in vitro. JOURNAL OF BACTERIOLOGY 179, 6133-6137.
DisqueKochem, C., & Dreiseikelmann, B., 1997. The cytoplasmic DNA-binding protein TraM binds to the inner membrane protein TraD in vitro. JOURNAL OF BACTERIOLOGY, 179(19), p 6133-6137.
C. DisqueKochem and B. Dreiseikelmann, “The cytoplasmic DNA-binding protein TraM binds to the inner membrane protein TraD in vitro”, JOURNAL OF BACTERIOLOGY, vol. 179, 1997, pp. 6133-6137.
DisqueKochem, C., Dreiseikelmann, B.: The cytoplasmic DNA-binding protein TraM binds to the inner membrane protein TraD in vitro. JOURNAL OF BACTERIOLOGY. 179, 6133-6137 (1997).
DisqueKochem, C, and Dreiseikelmann, Brigitte. “The cytoplasmic DNA-binding protein TraM binds to the inner membrane protein TraD in vitro”. JOURNAL OF BACTERIOLOGY 179.19 (1997): 6133-6137.

57 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

IHF protein inhibits cleavage but not assembly of plasmid R388 relaxosomes.
Moncalián G, Valle M, Valpuesta JM, de la Cruz F., Mol Microbiol 31(6), 1999
PMID: 10209739
Analysis of F factor TraD membrane topology by use of gene fusions and trypsin-sensitive insertions.
Lee MH, Kosuk N, Bailey J, Traxler B, Manoil C., J Bacteriol 181(19), 1999
PMID: 10498725
Characterization of ATP and DNA binding activities of TrwB, the coupling protein essential in plasmid R388 conjugation.
Moncalián G, Cabezón E, Alkorta I, Valle M, Moro F, Valpuesta JM, Goñi FM, de La Cruz F., J Biol Chem 274(51), 1999
PMID: 10593894
The essential transfer protein TraM binds to DNA as a tetramer.
Verdino P, Keller W, Strohmaier H, Bischof K, Lindner H, Koraimann G., J Biol Chem 274(52), 1999
PMID: 10601314

27 References

Daten bereitgestellt von Europe PubMed Central.

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PMID: 2198269
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PMID: 6111288

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