Matrix metalloproteinase-8 is expressed in rheumatoid synovial fibroblasts and endothelial cells - Regulation by tumor necrosis factor-alpha and doxycycline

Hanemaaijer R, Sorsa T, Konttinen YT, Ding YL, Sutinen M, Visser H, vanHinsbergh VWM, Helaakoski T, Kainulainen T, Ronka H, Tschesche H, et al. (1997)
JOURNAL OF BIOLOGICAL CHEMISTRY 272(50): 31504-31509.

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Abstract / Bemerkung
Neutrophil collagenase (matrix metalloproteinase-8 or MMP-8) is regarded as being synthesized exclusively by polymorphonuclear neutrophils (PMN). However, in vivo MMP-8 expression was observed in mononuclear fibroblast-like cells in the rheumatoid synovial membrane. In addition, we detected MMP-8 mRNA expression in cultured rheumatoid synovial fibroblasts and human endothelial cells. Up-regulation of MMP-8 was observed after treatment of the cells with either tumor necrosis factor-alpha (10 ng/ml) or phorbol 12-myristate 13-acetate (10 nM). Western analysis showed a similar regulation at the protein level. The size of secreted MMP-8 was 50 kDa, which is about 30 kDa smaller than MMP-8 from PMN. Conditioned media from rheumatoid synovial fibroblasts contained both type I and II collagen degrading activity. However, degradation of type II collagen, but not that of type I collagen, was completely inhibited by 50 mu M doxycycline, suggesting specific MMP-8 activity. In addition, doxycycline down-regulated MMP-8 induction, at both the mRNA and protein levels. Thus MMP-8 exerts markedly wider expression in human cells than had been thought previously, implying that PMN are not the only source of cartilage degrading activity at arthritic sites. The inhibition of both MMP-8 activity and synthesis by doxycycline provides an incentive for further studies on the clinical effects of doxycycline in the treatment of rheumatoid arthritis.
Erscheinungsjahr
Zeitschriftentitel
JOURNAL OF BIOLOGICAL CHEMISTRY
Band
272
Zeitschriftennummer
50
Seite
31504-31509
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Hanemaaijer R, Sorsa T, Konttinen YT, et al. Matrix metalloproteinase-8 is expressed in rheumatoid synovial fibroblasts and endothelial cells - Regulation by tumor necrosis factor-alpha and doxycycline. JOURNAL OF BIOLOGICAL CHEMISTRY. 1997;272(50):31504-31509.
Hanemaaijer, R., Sorsa, T., Konttinen, Y. T., Ding, Y. L., Sutinen, M., Visser, H., vanHinsbergh, V. W. M., et al. (1997). Matrix metalloproteinase-8 is expressed in rheumatoid synovial fibroblasts and endothelial cells - Regulation by tumor necrosis factor-alpha and doxycycline. JOURNAL OF BIOLOGICAL CHEMISTRY, 272(50), 31504-31509. doi:10.1074/jbc.272.50.31504
Hanemaaijer, R., Sorsa, T., Konttinen, Y. T., Ding, Y. L., Sutinen, M., Visser, H., vanHinsbergh, V. W. M., Helaakoski, T., Kainulainen, T., Ronka, H., et al. (1997). Matrix metalloproteinase-8 is expressed in rheumatoid synovial fibroblasts and endothelial cells - Regulation by tumor necrosis factor-alpha and doxycycline. JOURNAL OF BIOLOGICAL CHEMISTRY 272, 31504-31509.
Hanemaaijer, R., et al., 1997. Matrix metalloproteinase-8 is expressed in rheumatoid synovial fibroblasts and endothelial cells - Regulation by tumor necrosis factor-alpha and doxycycline. JOURNAL OF BIOLOGICAL CHEMISTRY, 272(50), p 31504-31509.
R. Hanemaaijer, et al., “Matrix metalloproteinase-8 is expressed in rheumatoid synovial fibroblasts and endothelial cells - Regulation by tumor necrosis factor-alpha and doxycycline”, JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 272, 1997, pp. 31504-31509.
Hanemaaijer, R., Sorsa, T., Konttinen, Y.T., Ding, Y.L., Sutinen, M., Visser, H., vanHinsbergh, V.W.M., Helaakoski, T., Kainulainen, T., Ronka, H., Tschesche, H., Salo, T.: Matrix metalloproteinase-8 is expressed in rheumatoid synovial fibroblasts and endothelial cells - Regulation by tumor necrosis factor-alpha and doxycycline. JOURNAL OF BIOLOGICAL CHEMISTRY. 272, 31504-31509 (1997).
Hanemaaijer, R, Sorsa, T, Konttinen, YT, Ding, YL, Sutinen, M, Visser, H, vanHinsbergh, VWM, Helaakoski, T, Kainulainen, T, Ronka, H, Tschesche, Harald, and Salo, T. “Matrix metalloproteinase-8 is expressed in rheumatoid synovial fibroblasts and endothelial cells - Regulation by tumor necrosis factor-alpha and doxycycline”. JOURNAL OF BIOLOGICAL CHEMISTRY 272.50 (1997): 31504-31509.

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Kähäri VM, Saarialho-Kere U., Ann Med 31(1), 1999
PMID: 10219712
Regulation of matrix metalloproteinase expression in tumor invasion.
Westermarck J, Kähäri VM., FASEB J 13(8), 1999
PMID: 10224222
Inhibition of the activities of matrix metalloproteinases 2, 8, and 9 by chlorhexidine.
Gendron R, Grenier D, Sorsa T, Mayrand D., Clin Diagn Lab Immunol 6(3), 1999
PMID: 10225852
Activation of neutrophil collagenase in periodontitis.
Romanelli R, Mancini S, Laschinger C, Overall CM, Sodek J, McCulloch CA., Infect Immun 67(5), 1999
PMID: 10225890
Signaling and effector pathways.
Müller-Ladner U, Gay RE, Gay S., Curr Opin Rheumatol 11(3), 1999
PMID: 10328579
Neutrophil granule proteins in bronchoalveolar lavage fluid from subjects with subclinical emphysema.
Betsuyaku T, Nishimura M, Takeyabu K, Tanino M, Venge P, Xu S, Kawakami Y., Am J Respir Crit Care Med 159(6), 1999
PMID: 10351949
The activation and function of host matrix metalloproteinases in dentin matrix breakdown in caries lesions.
Tjäderhane L, Larjava H, Sorsa T, Uitto VJ, Larmas M, Salo T., J Dent Res 77(8), 1998
PMID: 9719036
The synthesis and biological activity of a novel series of diazepine MMP inhibitors.
Levin JI, DiJoseph JF, Killar LM, Sung A, Walter T, Sharr MA, Roth CE, Skotnicki JS, Albright JD., Bioorg Med Chem Lett 8(19), 1998
PMID: 9873598
Inhibition of MMP synthesis by doxycycline and chemically modified tetracyclines (CMTs) in human endothelial cells.
Hanemaaijer R, Visser H, Koolwijk P, Sorsa T, Salo T, Golub LM, van Hinsbergh VW., Adv Dent Res 12(2), 1998
PMID: 9972133
The effects of chemically modified tetracyclines (CMTs) on human keratinocyte proliferation and migration.
Mäkelä M, Sorsa T, Uitto VJ, Salo T, Teronen O, Larjava H., Adv Dent Res 12(2), 1998
PMID: 9972137

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