Effects of dissolved oxygen levels and the role of extra- and intracellular amino acid concentrations upon the metabolism of mammalian cell lines during batch and continuous cultures

Heidemann R, Lütkemeyer D, Büntemeyer H, Lehmann J (1998)
CYTOTECHNOLOGY 26(3): 185-197.

Download
Es wurde kein Volltext hochgeladen. Nur Publikationsnachweis!
Zeitschriftenaufsatz | Veröffentlicht | Englisch
Autor
; ; ;
Abstract / Bemerkung
The effects of dissolved oxygen and the concentration of essential amino acids upon the metabolism of two mammalian cell lines (rCHO producing human active (t-PA) and a mouse-mouse hybridoma) were investigated in batch, chemostat, and perfusion cultures. Intracellular amino acid concentrations were measured for both cell lines during repeated batch cultures and the Ks-values for the essential amino acids were calculated using Monod equations via computer simulation. The Ks-values were in the range of 10 mmol L-1 and the pool of most intracellular amino acids remained constant at about 10-100 fold higher in concentration than in the medium. No significant differences were observed between the hybridoma and CHO cell. The specific nutrient uptake rates corresponded with the cell specific growth rate and the effects of reduced dissolved oxygen concentrations only became evident when the DO dropped below 5% of air saturation (critical concentration below 1%). Nevertheless, a correlation between nutrient concentration and specific oxygen uptake was detected.
Erscheinungsjahr
Zeitschriftentitel
CYTOTECHNOLOGY
Band
26
Zeitschriftennummer
3
Seite
185-197
ISSN
PUB-ID

Zitieren

Heidemann R, Lütkemeyer D, Büntemeyer H, Lehmann J. Effects of dissolved oxygen levels and the role of extra- and intracellular amino acid concentrations upon the metabolism of mammalian cell lines during batch and continuous cultures. CYTOTECHNOLOGY. 1998;26(3):185-197.
Heidemann, R., Lütkemeyer, D., Büntemeyer, H., & Lehmann, J. (1998). Effects of dissolved oxygen levels and the role of extra- and intracellular amino acid concentrations upon the metabolism of mammalian cell lines during batch and continuous cultures. CYTOTECHNOLOGY, 26(3), 185-197. doi:10.1023/A:1007917409455
Heidemann, R., Lütkemeyer, D., Büntemeyer, H., and Lehmann, J. (1998). Effects of dissolved oxygen levels and the role of extra- and intracellular amino acid concentrations upon the metabolism of mammalian cell lines during batch and continuous cultures. CYTOTECHNOLOGY 26, 185-197.
Heidemann, R., et al., 1998. Effects of dissolved oxygen levels and the role of extra- and intracellular amino acid concentrations upon the metabolism of mammalian cell lines during batch and continuous cultures. CYTOTECHNOLOGY, 26(3), p 185-197.
R. Heidemann, et al., “Effects of dissolved oxygen levels and the role of extra- and intracellular amino acid concentrations upon the metabolism of mammalian cell lines during batch and continuous cultures”, CYTOTECHNOLOGY, vol. 26, 1998, pp. 185-197.
Heidemann, R., Lütkemeyer, D., Büntemeyer, H., Lehmann, J.: Effects of dissolved oxygen levels and the role of extra- and intracellular amino acid concentrations upon the metabolism of mammalian cell lines during batch and continuous cultures. CYTOTECHNOLOGY. 26, 185-197 (1998).
Heidemann, R, Lütkemeyer, Dirk, Büntemeyer, Heino, and Lehmann, J. “Effects of dissolved oxygen levels and the role of extra- and intracellular amino acid concentrations upon the metabolism of mammalian cell lines during batch and continuous cultures”. CYTOTECHNOLOGY 26.3 (1998): 185-197.

16 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Rapid Generation and Detection of Biomimetic Oxygen Concentration Gradients In Vitro.
Khan DH, Roberts SA, Cressman JR, Agrawal N., Sci Rep 7(1), 2017
PMID: 29044222
Real-time monitoring of specific oxygen uptake rates of embryonic stem cells in a microfluidic cell culture device.
Super A, Jaccard N, Cardoso Marques MP, Macown RJ, Griffin LD, Veraitch FS, Szita N., Biotechnol J 11(9), 2016
PMID: 27214658
On the model-based optimization of secreting mammalian cell (GS-NS0) cultures.
Kiparissides A, Pistikopoulos EN, Mantalaris A., Biotechnol Bioeng 112(3), 2015
PMID: 25219609
Modeling kinetics of a large-scale fed-batch CHO cell culture by Markov chain Monte Carlo method.
Xing Z, Bishop N, Leister K, Li ZJ., Biotechnol Prog 26(1), 2010
PMID: 19834967
Scale-up analysis for a CHO cell culture process in large-scale bioreactors.
Xing Z, Kenty BM, Li ZJ, Lee SS., Biotechnol Bioeng 103(4), 2009
PMID: 19280669
Growth behavior of number distributed adherent MDCK cells for optimization in microcarrier cultures.
Bock A, Sann H, Schulze-Horsel J, Genzel Y, Reichl U, Möhler L., Biotechnol Prog 25(6), 2009
PMID: 19691122
A simple kinetic model for myeloma cell culture with consideration of lysine limitation.
Liu YH, Bi JX, Zeng AP, Yuan JQ., Bioprocess Biosyst Eng 31(6), 2008
PMID: 18253755
Cost-effective engineering of a small-scale bioreactor.
Bartholomew SR, Tansey JT., Biotechnol Bioeng 96(2), 2007
PMID: 16917951
Heterogeneous conditions in dissolved oxygen affect N-glycosylation but not productivity of a monoclonal antibody in hybridoma cultures.
Serrato JA, Palomares LA, Meneses-Acosta A, Ramírez OT., Biotechnol Bioeng 88(2), 2004
PMID: 15449295
Glucose-based optimization of CHO-cell perfusion cultures.
Dowd JE, Kwok KE, Piret JM., Biotechnol Bioeng 75(2), 2001
PMID: 11536149
Endogenous retrovirus particles and their repercussion effects on the growth behaviour of continuous hybridoma cultivation processes.
Hawerkamp A, Gudermann F, Falkenhain A, Luetkemeyer D, Kahmann U, Lehmann J., Cytotechnology 37(2), 2001
PMID: 19002905
The use of peptones as medium additives for the production of a recombinant therapeutic protein in high density perfusion cultures of mammalian cells.
Heidemann R, Zhang C, Qi H, Larrick Rule J, Rozales C, Park S, Chuppa S, Ray M, Michaels J, Konstantinov K, Naveh D., Cytotechnology 32(2), 2000
PMID: 19002977
Effects on growth behavior in continuous hybridoma cell cultures: The role of viral contamination.
Hawerkamp A, Lütkemeyer D, Gudermann F, Falkenhain A, Büntemeyer H, Lehmann J., Cytotechnology 28(1-3), 1998
PMID: 19003403

48 References

Daten bereitgestellt von Europe PubMed Central.

Production of tPA in recombinant CHO cells under oxygen-limited conditions.
Lin AA, Kimura R, Miller WM., Biotechnol. Bioeng. 42(3), 1993
PMID: 18613018
Enzymology of glutamine
Meister A., 1984
Induction of apoptosis in oxygen-deprived cultures of hybridoma cells.
Mercille S, Massie B., Cytotechnology 15(1-3), 1994
PMID: 7765924
Effects of dissolved oxygen concentration on hybridoma growth and metabolism in continuous culture.
Miller WM, Wilke CR, Blanch HW., J. Cell. Physiol. 132(3), 1987
PMID: 3654764

Monod J., 1942
Neutral amino acid transport systems of tissue culture cells.
Oxender DL, Lee M, Moore PA, Cecchini G., J. Biol. Chem. 252(8), 1977
PMID: 853037

Pirt SJ., 1985
The supply of oxygen to submerged cultures of BHK 21 cells.
Radlett PJ, Telling RC, Whitside JP, Maskell MA., Biotechnol. Bioeng. 14(3), 1972
PMID: 5063909
Production of monoclonal antibodies in culture
Reuveny S, Velez D, Riske F, Mcmillan JD, Miller L., 1985
Extra- and intracellular metabolite concentrations for murine hybridoma cells.
Schmid G, Blanch HW., Appl. Microbiol. Biotechnol. 36(5), 1992
PMID: 1368066
Transcription level of CHO cells influenced by amino acids
Seewöster T, Korioth F, Frey J, Lehmann J., 1995
Neutral amino acid transport systems in Chinese hamster ovary cells.
Shotwell MA, Jayme DW, Kilberg MS, Oxender DL., J. Biol. Chem. 256(11), 1981
PMID: 7240147
The influence of dissolved oxygen tension on the metabolic activity of an immobilized hybridoma population.
Thommes J, Gatgens J, Biselli M, Runstadler PW, Wandrey C., Cytotechnology 13(1), 1993
PMID: 7764605
Effects of cell density and glucose and glutamine levels on the respiration rates of hybridoma cells.
Wohlpart D, Kirwan D, Gainer J., Biotechnol. Bioeng. 36(6), 1990
PMID: 18595122

Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®

Quellen

PMID: 22358616
PubMed | Europe PMC

Suchen in

Google Scholar