Corynebacterium striatum chloramphenicol resistance transposon Tn5564: Genetic organization and transposition in Corynebacterium glutamicum

Tauch A, Zheng ZX, Pühler A, Kalinowski J (1998)
Plasmid 40(2): 126-139.

Journal Article | Published | English

No fulltext has been uploaded

Abstract
The clinical isolate Corynebacterium striatum M82B (formerly Corynebacterium xerosis M82B) carries the 50-kb R-plasmid pTP10 conferring resistance to the antibiotics chloramphenicol, erythromycin, kanamycin, and tetracycline. DNA sequence analysis of the chloramphenicol resistance region revealed the presence of the 4155-bp transposable element Tn5564. The ends of Tn5564 are identical 22-bp inverted repeats flanked by a 6-bp target site duplication. The central region of Tn5564 encodes the chloramphenicol resistance gene cmx, specifying a transmembrane chloramphenicol efflux protein, and an open reading frame homologous to transposases of insertion sequences identified in Arthrobacter nicotinovorans and Bordetella pertussis. Furthermore, the 1715-bp insertion sequence IS1513 encoding a putative transposase of the IS30 family is an integral part of Tn5564 and is located upstream of cmx For transposon mutagenesis, Tn5564 was transferred to Corynebacterium glutamicum on a mobilizable Escherichia coli plasmid using RP4-mediated intergeneric conjugation. Transposition of Tn5564 in C. glutamicum occurred with a frequency of 3.3 X 10(-8) and resulted in an insertion into target sites containing the central palindromic tetranucleotide CTAG. A Tn5564-induced mutant strain of C. glutamicum was found to carry the transposon in the ftsZ gene region. (C) 1998 Academic Press.
Publishing Year
ISSN
PUB-ID

Cite this

Tauch A, Zheng ZX, Pühler A, Kalinowski J. Corynebacterium striatum chloramphenicol resistance transposon Tn5564: Genetic organization and transposition in Corynebacterium glutamicum. Plasmid. 1998;40(2):126-139.
Tauch, A., Zheng, Z. X., Pühler, A., & Kalinowski, J. (1998). Corynebacterium striatum chloramphenicol resistance transposon Tn5564: Genetic organization and transposition in Corynebacterium glutamicum. Plasmid, 40(2), 126-139.
Tauch, A., Zheng, Z. X., Pühler, A., and Kalinowski, J. (1998). Corynebacterium striatum chloramphenicol resistance transposon Tn5564: Genetic organization and transposition in Corynebacterium glutamicum. Plasmid 40, 126-139.
Tauch, A., et al., 1998. Corynebacterium striatum chloramphenicol resistance transposon Tn5564: Genetic organization and transposition in Corynebacterium glutamicum. Plasmid, 40(2), p 126-139.
A. Tauch, et al., “Corynebacterium striatum chloramphenicol resistance transposon Tn5564: Genetic organization and transposition in Corynebacterium glutamicum”, Plasmid, vol. 40, 1998, pp. 126-139.
Tauch, A., Zheng, Z.X., Pühler, A., Kalinowski, J.: Corynebacterium striatum chloramphenicol resistance transposon Tn5564: Genetic organization and transposition in Corynebacterium glutamicum. Plasmid. 40, 126-139 (1998).
Tauch, Andreas, Zheng, ZX, Pühler, Alfred, and Kalinowski, Jörn. “Corynebacterium striatum chloramphenicol resistance transposon Tn5564: Genetic organization and transposition in Corynebacterium glutamicum”. Plasmid 40.2 (1998): 126-139.
This data publication is cited in the following publications:
This publication cites the following data publications:

27 Citations in Europe PMC

Data provided by Europe PubMed Central.

Complete Genome Sequence and Annotation of Corynebacterium singulare DSM 44357, Isolated from a Human Semen Specimen.
Merten M, Brinkrolf K, Albersmeier A, Kutter Y, Ruckert C, Tauch A., Genome Announc 3(2), 2015
PMID: 25814602
Classification of phenotypic subpopulations in isogenic bacterial cultures by triple promoter probing at single cell level.
Schluter JP, Czuppon P, Schauer O, Pfaffelhuber P, McIntosh M, Becker A., J. Biotechnol. 198(), 2015
PMID: 25661839

39 References

Data provided by Europe PubMed Central.

Prediction of helical transmembrane segments at 95% accuracy
Rost, Prot. Sci. 4(), 1995

Sambrook, 1989
DNA sequencing with chain-terminating inhibitors.
Sanger F, Nicklen S, Coulson AR., Proc. Natl. Acad. Sci. U.S.A. 74(12), 1977
PMID: 271968
High-frequency conjugal plasmid transfer from gram-negative Escherichia coli to various gram-positive coryneform bacteria.
Schafer A, Kalinowski J, Simon R, Seep-Feldhaus AH, Puhler A., J. Bacteriol. 172(3), 1990
PMID: 2106514
A broad host range mobilization system forin vivo
Simon, Bio/Technology 1(), 1983
The current status and portability of our sequence handling software.
Staden R., Nucleic Acids Res. 14(1), 1986
PMID: 3511446
Corynebacterium glutamicum DNA is subjected to methylation-restriction in Escherichia coli.
Tauch A, Kirchner O, Wehmeier L, Kalinowski J, Puhler A., FEMS Microbiol. Lett. 123(3), 1994
PMID: 7988915
A simplified protocol for fast plasmid DNA sequencing
Zimmermann, Nucleic Acids Res. 18(), 1990

Export

0 Marked Publications

Open Data PUB

Web of Science

View record in Web of Science®

Sources

PMID: 9735314
PubMed | Europe PMC

Search this title in

Google Scholar