LacZ transgene expression as a cell marker to analyse rescue from the 2-cell block in mouse aggregation chimeras

Neganova IE, Augustin M, Sekirina GG, Jockusch H (1998)
ZYGOTE 6(3): 223-226.

Download
No fulltext has been uploaded. References only!
DOI
Journal Article | Original Article | Published | English

No fulltext has been uploaded

Author
; ; ;
Department
Fakultät für Biologie
Abstract
Embryos from certain mouse strains are arrested at the 2-cell stage in cell culture ('2-cell block'), whereas those from other strains develop to the blastocyst stage under the same conditions. It was previously shown that blocking embryos can be rescued in culture by aggregation with an excess of 2-cell stages of a non-blocking strain such as CBA x C57BL/6 F-2. Here we have employed a LacZ transgene in a blocking strain (NMRI) to follow the fate of rescued blastomeres up to the blastocyst stage. We found that rescued blastomeres can participate in both inner cell mass and trophoblast formation, thus completely overcoming the 2-cell block.
Keywords
2-cell block ; aggregation chimeras ; cell labelling ; LacZ transgene ; preimplantation development ; expression
Publishing Year
1998
ISSN
0967-1994
PUB-ID

Cite this

Neganova IE, Augustin M, Sekirina GG, Jockusch H. LacZ transgene expression as a cell marker to analyse rescue from the 2-cell block in mouse aggregation chimeras. ZYGOTE. 1998;6(3):223-226.
Neganova, I. E., Augustin, M., Sekirina, G. G., & Jockusch, H. (1998). LacZ transgene expression as a cell marker to analyse rescue from the 2-cell block in mouse aggregation chimeras. ZYGOTE, 6(3), 223-226. doi:10.1017/S096719949800015X
Neganova, I. E., Augustin, M., Sekirina, G. G., and Jockusch, H. (1998). LacZ transgene expression as a cell marker to analyse rescue from the 2-cell block in mouse aggregation chimeras. ZYGOTE 6, 223-226.
Neganova, I.E., et al., 1998. LacZ transgene expression as a cell marker to analyse rescue from the 2-cell block in mouse aggregation chimeras. ZYGOTE, 6(3), p 223-226.
I.E. Neganova, et al., “LacZ transgene expression as a cell marker to analyse rescue from the 2-cell block in mouse aggregation chimeras”, ZYGOTE, vol. 6, 1998, pp. 223-226.
Neganova, I.E., Augustin, M., Sekirina, G.G., Jockusch, H.: LacZ transgene expression as a cell marker to analyse rescue from the 2-cell block in mouse aggregation chimeras. ZYGOTE. 6, 223-226 (1998).
Neganova, IE, Augustin, M, Sekirina, GG, and Jockusch, Harald. “LacZ transgene expression as a cell marker to analyse rescue from the 2-cell block in mouse aggregation chimeras”. ZYGOTE 6.3 (1998): 223-226.
This data publication is cited in the following publications:
This publication cites the following data publications:

5 Citations in Europe PMC

Data provided by Europe PubMed Central.

Global gene expression analysis of bovine blastocysts produced by multiple methods.
Zhou W, Xiang T, Walker S, Farrar V, Hwang E, Findeisen B, Sadeghieh S, Arenivas F, Abruzzese RV, Polejaeva I., Mol Reprod Dev 75(5), 2008
PMID: 17886272
Aggregation of bovine cloned embryos at the four-cell stage stimulated gene expression and in vitro embryo development.
Zhou W, Xiang T, Walker S, Abruzzese RV, Hwang E, Farrar V, Findeisen B, Sadeghieh S, Arenivas F, Chen SH, Polejaeva I., Mol Reprod Dev 75(8), 2008
PMID: 18196553
Aggregating embryonic but not somatic nuclear transfer embryos increases cloning efficiency in cattle.
Misica-Turner PM, Oback FC, Eichenlaub M, Wells DN, Oback B., Biol Reprod 76(2), 2007
PMID: 17050861
Subzonal older adult fibroblast insertion in both in vivo-fertilized and nuclear transfer rabbit zygotes and embryos: effects on further in vitro embryo development.
Cervera RP, Garcia-Ximénez F., Cloning Stem Cells 6(3), 2004
PMID: 15671676
Aberrant methylation patterns at the two-cell stage as an indicator of early developmental failure.
Shi W, Haaf T., Mol Reprod Dev 63(3), 2002
PMID: 12237948

Export

0 Marked Publications

Open Data PUB

Web of Science

View record in Web of Science®

Sources

PMID: 9854793
PubMed | Europe PMC

Search this title in

Google Scholar