LacZ transgene expression as a cell marker to analyse rescue from the 2-cell block in mouse aggregation chimeras

Neganova, IE; Augustin, M; Sekirina, GG; Jockusch, Harald

LacZ transgene expression as a cell marker to analyse rescue from the 2-cell block in mouse aggregation chimeras

Neganova IE, Augustin M, Sekirina GG, Jockusch H (1998)
ZYGOTE 6(3): 223-226.

DOI

10.1017/S096719949800015X

Journal Article | Published | English

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Author

Neganova, IE ; Augustin, M ; Sekirina, GG ; Jockusch, Harald^UniBi

Department

Fakultät für Biologie

Abstract

Embryos from certain mouse strains are arrested at the 2-cell stage in cell culture ('2-cell block'), whereas those from other strains develop to the blastocyst stage under the same conditions. It was previously shown that blocking embryos can be rescued in culture by aggregation with an excess of 2-cell stages of a non-blocking strain such as CBA x C57BL/6 F-2. Here we have employed a LacZ transgene in a blocking strain (NMRI) to follow the fate of rescued blastomeres up to the blastocyst stage. We found that rescued blastomeres can participate in both inner cell mass and trophoblast formation, thus completely overcoming the 2-cell block.

Keywords

2-cell block ; aggregation chimeras ; cell labelling ; LacZ transgene ; preimplantation development ; expression

Publishing Year

1998

ISSN

0967-1994

PUB-ID

1623741

Cite this

Neganova IE, Augustin M, Sekirina GG, Jockusch H. LacZ transgene expression as a cell marker to analyse rescue from the 2-cell block in mouse aggregation chimeras. ZYGOTE. 1998;6(3):223-226.

Neganova, I. E., Augustin, M., Sekirina, G. G., & Jockusch, H. (1998). LacZ transgene expression as a cell marker to analyse rescue from the 2-cell block in mouse aggregation chimeras. ZYGOTE, 6(3), 223-226.

Neganova, I. E., Augustin, M., Sekirina, G. G., and Jockusch, H. (1998). LacZ transgene expression as a cell marker to analyse rescue from the 2-cell block in mouse aggregation chimeras. ZYGOTE 6, 223-226.

Neganova, I.E., et al., 1998. LacZ transgene expression as a cell marker to analyse rescue from the 2-cell block in mouse aggregation chimeras. ZYGOTE, 6(3), p 223-226.

I.E. Neganova, et al., “LacZ transgene expression as a cell marker to analyse rescue from the 2-cell block in mouse aggregation chimeras”, ZYGOTE, vol. 6, 1998, pp. 223-226.

Neganova, I.E., Augustin, M., Sekirina, G.G., Jockusch, H.: LacZ transgene expression as a cell marker to analyse rescue from the 2-cell block in mouse aggregation chimeras. ZYGOTE. 6, 223-226 (1998).

Neganova, IE, Augustin, M, Sekirina, GG, and Jockusch, Harald. “LacZ transgene expression as a cell marker to analyse rescue from the 2-cell block in mouse aggregation chimeras”. ZYGOTE 6.3 (1998): 223-226.

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