Expression of the Corynebacterium glutamicum panD gene encoding L-aspartate-alpha-decarboxylase leads to pantothenate overproduction in Escherichia coli

Dusch N, Pühler A, Kalinowski J (1999)
Appl Environ Microbiol 65(4): 1530-1539.

Download
No fulltext has been uploaded. References only!
Journal Article | Original Article | Published | English

No fulltext has been uploaded

Abstract
The Corynebacterium glutamicum panD gene was identified by functional complementation of an Escherichia coli panD mutant strain. Sequence analysis revealed that the coding region of panD comprises 411 bp and specifies a protein of 136 amino acid residues with a deduced molecular mass of 14.1 kDa. A defined C. glutamicum panD mutant completely lacked L-aspartate-alpha-decarboxylase activity and exhibited beta-alanine auxotrophy, The C. glutamicum panD (panD(C.g.)) as well as the E. coli panD (panD(E.c.)) genes were cloned into a bifunctional expression plasmid to allow gene analysis in C. glutamicum as well as in E. coli. The enhanced expression of panD(C.g.) in C. glutamicum resulted in the formation of two distinct proteins In sodium dodecyl sulfate-polyacrylamide gel electrophoresis, leading to the assumption that the panD(C.g.) gene product is proteolytically processed into two subunits, By increased expression of panD(C.g.) in C. glutamicum, the activity of L-aspartate-alpha-decarboxylase was 288-fold increased, whereas the panD(E.c.) gene resulted only in a 4-fold enhancement. The similar experiment performed in E. coli revealed that panD(C.g.) achieved a 41-fold increase and that panD(E.c.) achieved a 3-fold increase of enzyme activity. The effect of the panD(C.g.) and panD(E.c.) gene expression in E, coli was studied with a view to pantothenate accumulation. Only by expression of the panD(C.g.) gene was sufficient p-alanine produced to abolish its limiting effect on pantothenate production. In cultures expressing the panD(E.c.) gene, the maximal pantothenate production was still dependent on external beta-alanine supplementation. The enhanced expression of panD(C.g.) in E. coli yielded the highest amount of pantothenate in the culture medium, with a specific productivity of 140 ng of pantothenate mg (dry weight)(-1) h(-1).
Publishing Year
ISSN
PUB-ID

Cite this

Dusch N, Pühler A, Kalinowski J. Expression of the Corynebacterium glutamicum panD gene encoding L-aspartate-alpha-decarboxylase leads to pantothenate overproduction in Escherichia coli. Appl Environ Microbiol. 1999;65(4):1530-1539.
Dusch, N., Pühler, A., & Kalinowski, J. (1999). Expression of the Corynebacterium glutamicum panD gene encoding L-aspartate-alpha-decarboxylase leads to pantothenate overproduction in Escherichia coli. Appl Environ Microbiol, 65(4), 1530-1539.
Dusch, N., Pühler, A., and Kalinowski, J. (1999). Expression of the Corynebacterium glutamicum panD gene encoding L-aspartate-alpha-decarboxylase leads to pantothenate overproduction in Escherichia coli. Appl Environ Microbiol 65, 1530-1539.
Dusch, N., Pühler, A., & Kalinowski, J., 1999. Expression of the Corynebacterium glutamicum panD gene encoding L-aspartate-alpha-decarboxylase leads to pantothenate overproduction in Escherichia coli. Appl Environ Microbiol, 65(4), p 1530-1539.
N. Dusch, A. Pühler, and J. Kalinowski, “Expression of the Corynebacterium glutamicum panD gene encoding L-aspartate-alpha-decarboxylase leads to pantothenate overproduction in Escherichia coli”, Appl Environ Microbiol, vol. 65, 1999, pp. 1530-1539.
Dusch, N., Pühler, A., Kalinowski, J.: Expression of the Corynebacterium glutamicum panD gene encoding L-aspartate-alpha-decarboxylase leads to pantothenate overproduction in Escherichia coli. Appl Environ Microbiol. 65, 1530-1539 (1999).
Dusch, N, Pühler, Alfred, and Kalinowski, Jörn. “Expression of the Corynebacterium glutamicum panD gene encoding L-aspartate-alpha-decarboxylase leads to pantothenate overproduction in Escherichia coli”. Appl Environ Microbiol 65.4 (1999): 1530-1539.
This data publication is cited in the following publications:
This publication cites the following data publications:

47 Citations in Europe PMC

Data provided by Europe PubMed Central.

Regulation of ldh expression during biotin-limited growth of Corynebacterium glutamicum.
Dietrich C, Nato A, Bost B, Le Maréchal P, Guyonvarch A., Microbiology 155(pt 4), 2009
PMID: 19332837
Disclosure of the mycobacterial outer membrane: cryo-electron tomography and vitreous sections reveal the lipid bilayer structure.
Hoffmann C, Leis A, Niederweis M, Plitzko JM, Engelhardt H., Proc Natl Acad Sci U S A 105(10), 2008
PMID: 18316738
Partial redundancy in the synthesis of the D-arabinose incorporated in the cell wall arabinan of Corynebacterineae.
Meniche X, de Sousa-d'Auria C, Van-der-Rest B, Bhamidi S, Huc E, Huang H, De Paepe D, Tropis M, McNeil M, Daffé M, Houssin C., Microbiology 154(pt 8), 2008
PMID: 18667564
Biosynthesis of Pantothenic Acid and Coenzyme A.
Leonardi R, Jackowski S., EcoSal Plus 2(2), 2007
PMID: 26443589
Roles of vitamins B5, B8, B9, B12 and molybdenum cofactor at cellular and organismal levels.
Rébeillé F, Ravanel S, Marquet A, Mendel RR, Webb ME, Smith AG, Warren MJ., Nat Prod Rep 24(5), 2007
PMID: 17898891
Elucidating biosynthetic pathways for vitamins and cofactors.
Webb ME, Marquet A, Mendel RR, Rébeillé F, Smith AG., Nat Prod Rep 24(5), 2007
PMID: 17898894
Pantothenate biosynthesis in higher plants: advances and challenges.
Chakauya Ereck, Coxon KatyM, Whitney HeatherM, Ashurst JenniferL, Abell Chris, Smith AlisonG., Physiol Plant 126(3), 2006
PMID: IND43783143
Rational design of a Corynebacterium glutamicum pantothenate production strain and its characterization by metabolic flux analysis and genome-wide transcriptional profiling.
Hüser AT, Chassagnole C, Lindley ND, Merkamm M, Guyonvarch A, Elisáková V, Pátek M, Kalinowski J, Brune I, Pühler A, Tauch A., Appl Environ Microbiol 71(6), 2005
PMID: 15933028
A polyketide synthase catalyzes the last condensation step of mycolic acid biosynthesis in mycobacteria and related organisms.
Portevin D, De Sousa-D'Auria C, Houssin C, Grimaldi C, Chami M, Daffé M, Guilhot C., Proc Natl Acad Sci U S A 101(1), 2004
PMID: 14695899
The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of L-aspartate-derived amino acids and vitamins.
Kalinowski J, Bathe B, Bartels D, Bischoff N, Bott M, Burkovski A, Dusch N, Eggeling L, Eikmanns BJ, Gaigalat L, Goesmann A, Hartmann M, Huthmacher K, Krämer R, Linke B, McHardy AC, Meyer F, Möckel B, Pfefferle W, Pühler A, Rey DA, Rückert C, Rupp O, Sahm H, Wendisch VF, Wiegräbe I, Tauch A., J Biotechnol 104(1-3), 2003
PMID: 12948626
Ketopantoate reductase activity is only encoded by ilvC in Corynebacterium glutamicum.
Merkamm M, Chassagnole C, Lindley ND, Guyonvarch A., J Biotechnol 104(1-3), 2003
PMID: 12948643

41 References

Data provided by Europe PubMed Central.

A broad host range mobilization system for in vivo genetic engineering: transposon mutagenesis in gram-negative bacteria
Simon R, Priefer U, Pühler A., 1983
Dihydrouracil as a growth factor for mutant strains of Escherichia coli.
SLOTNICK IJ, WEINFELD H., J. Bacteriol. 74(2), 1957
PMID: 13475206

Smith R., 1988
The current status and portability of our sequence handling software.
Staden R., Nucleic Acids Res. 14(1), 1986
PMID: 3511446
Use of synthetic oligodeoxyribonucleotides for the isolation of specific cloned DNA sequences
Suggs S, Hirose T, Miyake T, Kawashima E, Johnson M, Itakura K, Wallace R., 1981
Corynebacterium glutamicum DNA is subjected to methylation-restriction in Escherichia coli.
Tauch A, Kirchner O, Wehmeier L, Kalinowski J, Puhler A., FEMS Microbiol. Lett. 123(3), 1994
PMID: 7988915
Acyl group transfer
Vagelos P., 1973
Pyruvoyl-dependent enzymes.
van Poelje PD, Snell EE., Annu. Rev. Biochem. 59(), 1990
PMID: 2197977
Acetylornithinase of Escherichia coli: partial purification and some properties.
VOGEL HJ, BONNER DM., J. Biol. Chem. 218(1), 1956
PMID: 13278318
A simplified protocol for fast plasmid DNA sequencing.
Zimmermann J, Voss H, Schwager C, Stegemann J, Erfle H, Stucky K, Kristensen T, Ansorge W., Nucleic Acids Res. 18(4), 1990
PMID: 2315028

Export

0 Marked Publications

Open Data PUB

Web of Science

View record in Web of Science®

Sources

PMID: 10103247
PubMed | Europe PMC

Search this title in

Google Scholar