A multicopy c-myc transgene as a nuclear label: Overgrowth of Myc(tg50) cells in allophenic mice

Augustin M, Klopp N, Ewald K, Jockusch H (1998)
Cell biology international 22(6): 401-411.

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To trace cell lineages and the origin and fate of cells in transplantation and embryo chimeras, a DNA/DNA in situ hybridization cell labelling system was developed, based on a 50-copy murine c-myc transgene on mouse chromosome 8. Elevated levels of cMyc mRNA were found in Myc*tg50 (Myc(tg50)/0 and Myc(tg50)/Myc(tg50)) transgenic tissues, but adult transgenic NMRI mice were anatomically and histologically indistinguishable from control NMRI mice and did not develop tumours on a wild-type or nude (nu/nu) background. The hybridization label detected transgenic nuclei with an efficiency of similar to 80%. In muscle grafts, this transgene label was successfully applied to trace donor cells in a labelled host and to study the invasion of a graft by host cells. When the cMyc hybridization was used in allophenic mice of the control <----> NMRI-Myc(tg50)/? (nu/+ or +/+) type, an up to a three-fold excess of MYC*tg50 positive over control nuclei was found in all organs examined (ventricle, skeletal muscle, liver, small intestine). This overgrowth of MYC*tg50 cells is probably due to transgene expression. Four out of seven (C57BL/6 x BALB/c) or (C57BL/6 x NMRI)<----> MYC*tg50 allophenic mice displayed anatomical abnormalities, e.g. an enlarged thymus and a tumour in the groin region. As these abnormalities were only observed in allophenic mice, they might be due to the imbalance of growth potential between MYC*tg50 transgenic and normal cells in the same individual. (C) 1998 Academic Press.
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Augustin M, Klopp N, Ewald K, Jockusch H. A multicopy c-myc transgene as a nuclear label: Overgrowth of Myc(tg50) cells in allophenic mice. Cell biology international. 1998;22(6):401-411.
Augustin, M., Klopp, N., Ewald, K., & Jockusch, H. (1998). A multicopy c-myc transgene as a nuclear label: Overgrowth of Myc(tg50) cells in allophenic mice. Cell biology international, 22(6), 401-411.
Augustin, M., Klopp, N., Ewald, K., and Jockusch, H. (1998). A multicopy c-myc transgene as a nuclear label: Overgrowth of Myc(tg50) cells in allophenic mice. Cell biology international 22, 401-411.
Augustin, M., et al., 1998. A multicopy c-myc transgene as a nuclear label: Overgrowth of Myc(tg50) cells in allophenic mice. Cell biology international, 22(6), p 401-411.
M. Augustin, et al., “A multicopy c-myc transgene as a nuclear label: Overgrowth of Myc(tg50) cells in allophenic mice”, Cell biology international, vol. 22, 1998, pp. 401-411.
Augustin, M., Klopp, N., Ewald, K., Jockusch, H.: A multicopy c-myc transgene as a nuclear label: Overgrowth of Myc(tg50) cells in allophenic mice. Cell biology international. 22, 401-411 (1998).
Augustin, M, Klopp, N, Ewald, K, and Jockusch, Harald. “A multicopy c-myc transgene as a nuclear label: Overgrowth of Myc(tg50) cells in allophenic mice”. Cell biology international 22.6 (1998): 401-411.
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Lessons from mouse chimaera experiments with a reiterated transgene marker: revised marker criteria and a review of chimaera markers.
Keighren MA, Flockhart J, Hodson BA, Shen GY, Birtley JR, Notarnicola-Harwood A, West JD., Transgenic Res. 24(4), 2015
PMID: 26048593

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