Role of phosphorylation and physiological state in the regulation of the muscular chloride channel CIC-1: A voltage-clamp study on isolated M-interosseus fibers

Chen MF, Jockusch H (1999)
Biochemical and biophysical research communications 261(2): 528-533.

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Zeitschriftenaufsatz | Veröffentlicht | Englisch
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Abstract / Bemerkung
Chloride currents (I-Cl) were investigated with the two-electrode voltage-clamp technique in enzymatically isolated fibers from interosseus muscles of wildtype (WT), denervated WT, and myotonic (ADR, ClC-1-deficient) mice. Characteristics of I-Cl were consistent with previous observations on rat muscle fibers and cultured nonmuscle cells transfected with hClC-1 cDNA. In the presence of 0.1 mM anthracene-9-carboxylic acid and in ADR fibers, I-Cl was reduced by > 90%. WT interosseus fibers denervated 6-7 days prior to isolation showed similar to 50% I-Cl compared to control fibers. Addition of 3.3 mu M staurosporine, a nonspecific inhibitor of protein kinases, increased I-Cl. in WT interosseus fibers by a factor of approximately two and altered its kinetic characteristics, We conclude that in dissociated fibers cultured for 1-2 days, in contrast to freshly isolated muscles, chloride conductance is downregulated by a mechanism involving protein phosphorylation. In situ this short-term regulation may complement transcriptional long-term regulation Of ClC-1. (C) 1999 Academic Press.
Erscheinungsjahr
Zeitschriftentitel
Biochemical and biophysical research communications
Band
261
Zeitschriftennummer
2
Seite
528-533
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PUB-ID

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Chen MF, Jockusch H. Role of phosphorylation and physiological state in the regulation of the muscular chloride channel CIC-1: A voltage-clamp study on isolated M-interosseus fibers. Biochemical and biophysical research communications. 1999;261(2):528-533.
Chen, M. F., & Jockusch, H. (1999). Role of phosphorylation and physiological state in the regulation of the muscular chloride channel CIC-1: A voltage-clamp study on isolated M-interosseus fibers. Biochemical and biophysical research communications, 261(2), 528-533. doi:10.1006/bbrc.1999.1061
Chen, M. F., and Jockusch, H. (1999). Role of phosphorylation and physiological state in the regulation of the muscular chloride channel CIC-1: A voltage-clamp study on isolated M-interosseus fibers. Biochemical and biophysical research communications 261, 528-533.
Chen, M.F., & Jockusch, H., 1999. Role of phosphorylation and physiological state in the regulation of the muscular chloride channel CIC-1: A voltage-clamp study on isolated M-interosseus fibers. Biochemical and biophysical research communications, 261(2), p 528-533.
M.F. Chen and H. Jockusch, “Role of phosphorylation and physiological state in the regulation of the muscular chloride channel CIC-1: A voltage-clamp study on isolated M-interosseus fibers”, Biochemical and biophysical research communications, vol. 261, 1999, pp. 528-533.
Chen, M.F., Jockusch, H.: Role of phosphorylation and physiological state in the regulation of the muscular chloride channel CIC-1: A voltage-clamp study on isolated M-interosseus fibers. Biochemical and biophysical research communications. 261, 528-533 (1999).
Chen, MF, and Jockusch, Harald. “Role of phosphorylation and physiological state in the regulation of the muscular chloride channel CIC-1: A voltage-clamp study on isolated M-interosseus fibers”. Biochemical and biophysical research communications 261.2 (1999): 528-533.

6 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Protein kinase C-dependent regulation of ClC-1 channels in active human muscle and its effect on fast and slow gating.
Riisager A, de Paoli FV, Yu WP, Pedersen TH, Chen TY, Nielsen OB., J Physiol 594(12), 2016
PMID: 26857341
Muscle chloride channel dysfunction in two mouse models of myotonic dystrophy.
Lueck JD, Mankodi A, Swanson MS, Thornton CA, Dirksen RT., J Gen Physiol 129(1), 2007
PMID: 17158949
Disuse of rat muscle in vivo reduces protein kinase C activity controlling the sarcolemma chloride conductance.
Pierno S, Desaphy JF, Liantonio A, De Luca A, Zarrilli A, Mastrofrancesco L, Procino G, Valenti G, Conte Camerino D., J Physiol 584(pt 3), 2007
PMID: 17855757
Regulated sarcolemmal localization of the muscle-specific ClC-1 chloride channel.
Papponen H, Kaisto T, Myllylä VV, Myllylä R, Metsikkö K., Exp Neurol 191(1), 2005
PMID: 15589523

36 References

Daten bereitgestellt von Europe PubMed Central.


Chen, Pflüger's Arch. 435(), 1998

Chen, J. Physiol. (London) 504(), 1997
PKC activity modulates availability and long openings of L-type Ca2+ channels in A7r5 cells.
Obejero-Paz CA, Auslender M, Scarpa A., Am. J. Physiol. 275(2 Pt 1), 1998
PMID: 9688608
Diacylglycerol/protein kinase C signalling: a mechanism for insulin resistance?
Shmueli E, Alberti KG, Record CO., J. Intern. Med. 234(4), 1993
PMID: 8409836

Kehne, Biol. Chem. Hoppe-Seyer 377(), 1996

Rosenbohm, J. Physiol. (London) 514(), 1999

Conte, Pflüger's Arch. 413(), 1989
The influence of inactivity on membrane resting conductances of rat skeletal muscle fibres undergoing reinnervation.
Conte-Camerino D, Bryant SH, Lograno MD, Mambrini M., J. Exp. Biol. 115(), 1985
PMID: 4031784

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