Role of phosphorylation and physiological state in the regulation of the muscular chloride channel CIC-1: A voltage-clamp study on isolated M-interosseus fibers

Chen MF, Jockusch H (1999)
Biochemical and biophysical research communications 261(2): 528-533.

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Chloride currents (I-Cl) were investigated with the two-electrode voltage-clamp technique in enzymatically isolated fibers from interosseus muscles of wildtype (WT), denervated WT, and myotonic (ADR, ClC-1-deficient) mice. Characteristics of I-Cl were consistent with previous observations on rat muscle fibers and cultured nonmuscle cells transfected with hClC-1 cDNA. In the presence of 0.1 mM anthracene-9-carboxylic acid and in ADR fibers, I-Cl was reduced by > 90%. WT interosseus fibers denervated 6-7 days prior to isolation showed similar to 50% I-Cl compared to control fibers. Addition of 3.3 mu M staurosporine, a nonspecific inhibitor of protein kinases, increased I-Cl. in WT interosseus fibers by a factor of approximately two and altered its kinetic characteristics, We conclude that in dissociated fibers cultured for 1-2 days, in contrast to freshly isolated muscles, chloride conductance is downregulated by a mechanism involving protein phosphorylation. In situ this short-term regulation may complement transcriptional long-term regulation Of ClC-1. (C) 1999 Academic Press.
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Chen MF, Jockusch H. Role of phosphorylation and physiological state in the regulation of the muscular chloride channel CIC-1: A voltage-clamp study on isolated M-interosseus fibers. Biochemical and biophysical research communications. 1999;261(2):528-533.
Chen, M. F., & Jockusch, H. (1999). Role of phosphorylation and physiological state in the regulation of the muscular chloride channel CIC-1: A voltage-clamp study on isolated M-interosseus fibers. Biochemical and biophysical research communications, 261(2), 528-533. doi:10.1006/bbrc.1999.1061
Chen, M. F., and Jockusch, H. (1999). Role of phosphorylation and physiological state in the regulation of the muscular chloride channel CIC-1: A voltage-clamp study on isolated M-interosseus fibers. Biochemical and biophysical research communications 261, 528-533.
Chen, M.F., & Jockusch, H., 1999. Role of phosphorylation and physiological state in the regulation of the muscular chloride channel CIC-1: A voltage-clamp study on isolated M-interosseus fibers. Biochemical and biophysical research communications, 261(2), p 528-533.
M.F. Chen and H. Jockusch, “Role of phosphorylation and physiological state in the regulation of the muscular chloride channel CIC-1: A voltage-clamp study on isolated M-interosseus fibers”, Biochemical and biophysical research communications, vol. 261, 1999, pp. 528-533.
Chen, M.F., Jockusch, H.: Role of phosphorylation and physiological state in the regulation of the muscular chloride channel CIC-1: A voltage-clamp study on isolated M-interosseus fibers. Biochemical and biophysical research communications. 261, 528-533 (1999).
Chen, MF, and Jockusch, Harald. “Role of phosphorylation and physiological state in the regulation of the muscular chloride channel CIC-1: A voltage-clamp study on isolated M-interosseus fibers”. Biochemical and biophysical research communications 261.2 (1999): 528-533.
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Protein kinase C-dependent regulation of ClC-1 channels in active human muscle and its effect on fast and slow gating.
Riisager A, de Paoli FV, Yu WP, Pedersen TH, Chen TY, Nielsen OB., J. Physiol. (Lond.) 594(12), 2016
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The muscle chloride channel ClC-1 is not directly regulated by intracellular ATP.
Zifarelli G, Pusch M., J. Gen. Physiol. 131(2), 2008
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Disuse of rat muscle in vivo reduces protein kinase C activity controlling the sarcolemma chloride conductance.
Pierno S, Desaphy JF, Liantonio A, De Luca A, Zarrilli A, Mastrofrancesco L, Procino G, Valenti G, Conte Camerino D., J. Physiol. (Lond.) 584(Pt 3), 2007
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Muscle chloride channel dysfunction in two mouse models of myotonic dystrophy.
Lueck JD, Mankodi A, Swanson MS, Thornton CA, Dirksen RT., J. Gen. Physiol. 129(1), 2007
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Regulated sarcolemmal localization of the muscle-specific ClC-1 chloride channel.
Papponen H, Kaisto T, Myllyla VV, Myllyla R, Metsikko K., Exp. Neurol. 191(1), 2005
PMID: 15589523

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