Nuclear and cytoplasmic maturation of mouse oocytes after treatment with synthetic meiosis-activating sterol in vitro

Hegele-Hartung C, Kuhnke J, Lessl M, Grondahl C, Ottesen J, Beier HM, Eisner S, Eichenlaub-Ritter U (1999)
BIOLOGY OF REPRODUCTION 61(5): 1362-1372.

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Abstract
Synthetically produced meiosis-activating sterol, a sterol originally derived from follicular fluid (FF-MAS), induces meiotic maturation of mouse oocytes in vitro. We therefore compared FF-MAS-induced maturation of naked mouse oocytes arrested in prophase I by either hypoxanthine (Hx) or forskolin (Fo) with spontaneous maturation of naked oocytes. FF-MAS-treated oocytes overcame the meiotic block by Hx or Fo, although germinal vesicle breakdown was delayed by 11 h and 7 h, respectively. We also investigated the influence of FF-MAS on chromosome, microtubule, and ultrastructural dynamics in Hx-cultured oocytes by immunocytochemistry and electron microscopy. Similarly to spontaneously matured oocytes, chromosomes became aligned, a barrel-shaped spindle formed, and overall organelle distribution was normal in FF-MAS-matured oocytes. The number of small cytoplasmic asters was elevated in FF-MAS-treated oocytes. Although the number of cortical granules (CGs) was similar to that in spontaneously matured oocytes, the overall distance between CGs and oolemma was increased in the FF-MAS group. These observations suggest that the initiation of meiotic maturation in FF-MAS-treated oocytes in the presence of high cAMP levels leads to a delayed but otherwise normal nuclear maturation. FF-MAS appears to improve oocyte quality by supporting microtubule assembly and by delaying CG release, which is known to contribute to reduced fertilization.
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Hegele-Hartung C, Kuhnke J, Lessl M, et al. Nuclear and cytoplasmic maturation of mouse oocytes after treatment with synthetic meiosis-activating sterol in vitro. BIOLOGY OF REPRODUCTION. 1999;61(5):1362-1372.
Hegele-Hartung, C., Kuhnke, J., Lessl, M., Grondahl, C., Ottesen, J., Beier, H. M., Eisner, S., et al. (1999). Nuclear and cytoplasmic maturation of mouse oocytes after treatment with synthetic meiosis-activating sterol in vitro. BIOLOGY OF REPRODUCTION, 61(5), 1362-1372.
Hegele-Hartung, C., Kuhnke, J., Lessl, M., Grondahl, C., Ottesen, J., Beier, H. M., Eisner, S., and Eichenlaub-Ritter, U. (1999). Nuclear and cytoplasmic maturation of mouse oocytes after treatment with synthetic meiosis-activating sterol in vitro. BIOLOGY OF REPRODUCTION 61, 1362-1372.
Hegele-Hartung, C., et al., 1999. Nuclear and cytoplasmic maturation of mouse oocytes after treatment with synthetic meiosis-activating sterol in vitro. BIOLOGY OF REPRODUCTION, 61(5), p 1362-1372.
C. Hegele-Hartung, et al., “Nuclear and cytoplasmic maturation of mouse oocytes after treatment with synthetic meiosis-activating sterol in vitro”, BIOLOGY OF REPRODUCTION, vol. 61, 1999, pp. 1362-1372.
Hegele-Hartung, C., Kuhnke, J., Lessl, M., Grondahl, C., Ottesen, J., Beier, H.M., Eisner, S., Eichenlaub-Ritter, U.: Nuclear and cytoplasmic maturation of mouse oocytes after treatment with synthetic meiosis-activating sterol in vitro. BIOLOGY OF REPRODUCTION. 61, 1362-1372 (1999).
Hegele-Hartung, C, Kuhnke, J, Lessl, M, Grondahl, C, Ottesen, J, Beier, HM, Eisner, S, and Eichenlaub-Ritter, Ursula. “Nuclear and cytoplasmic maturation of mouse oocytes after treatment with synthetic meiosis-activating sterol in vitro”. BIOLOGY OF REPRODUCTION 61.5 (1999): 1362-1372.
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Wang C, Xie H, Song X, Ning G, Yan J, Chen X, Xu B, Ouyang H, Xia G., Theriogenology 66(5), 2006
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Cao X, Pomerantz SH, Popliker M, Tsafriri A., Biol. Reprod. 71(6), 2004
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Roles of gonadotropins and meiosis-activating sterols in meiotic resumption of cultured follicle-enclosed mouse oocytes.
Xie H, Xia G, Byskov AG, Andersen CY, Bo S, Tao Y., Mol. Cell. Endocrinol. 218(1-2), 2004
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Physiology of meiosis-activating sterol: endogenous formation and mode of action.
Grondahl C, Breinholt J, Wahl P, Murray A, Hansen TH, Faerge I, Stidsen CE, Raun K, Hegele-Hartung C., Hum. Reprod. 18(1), 2003
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Cavilla JL, Kennedy CR, Baltsen M, Klentzeris LD, Byskov AG, Hartshorne GM., Hum. Reprod. 16(3), 2001
PMID: 11228227

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