Nuclear and cytoplasmic maturation of mouse oocytes after treatment with synthetic meiosis-activating sterol in vitro

Hegele-Hartung C, Kuhnke J, Lessl M, Grondahl C, Ottesen J, Beier HM, Eisner S, Eichenlaub-Ritter U (1999)
BIOLOGY OF REPRODUCTION 61(5): 1362-1372.

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Zeitschriftenaufsatz | Veröffentlicht | Englisch
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Abstract / Bemerkung
Synthetically produced meiosis-activating sterol, a sterol originally derived from follicular fluid (FF-MAS), induces meiotic maturation of mouse oocytes in vitro. We therefore compared FF-MAS-induced maturation of naked mouse oocytes arrested in prophase I by either hypoxanthine (Hx) or forskolin (Fo) with spontaneous maturation of naked oocytes. FF-MAS-treated oocytes overcame the meiotic block by Hx or Fo, although germinal vesicle breakdown was delayed by 11 h and 7 h, respectively. We also investigated the influence of FF-MAS on chromosome, microtubule, and ultrastructural dynamics in Hx-cultured oocytes by immunocytochemistry and electron microscopy. Similarly to spontaneously matured oocytes, chromosomes became aligned, a barrel-shaped spindle formed, and overall organelle distribution was normal in FF-MAS-matured oocytes. The number of small cytoplasmic asters was elevated in FF-MAS-treated oocytes. Although the number of cortical granules (CGs) was similar to that in spontaneously matured oocytes, the overall distance between CGs and oolemma was increased in the FF-MAS group. These observations suggest that the initiation of meiotic maturation in FF-MAS-treated oocytes in the presence of high cAMP levels leads to a delayed but otherwise normal nuclear maturation. FF-MAS appears to improve oocyte quality by supporting microtubule assembly and by delaying CG release, which is known to contribute to reduced fertilization.
Erscheinungsjahr
Zeitschriftentitel
BIOLOGY OF REPRODUCTION
Band
61
Zeitschriftennummer
5
Seite
1362-1372
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eISSN
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Hegele-Hartung C, Kuhnke J, Lessl M, et al. Nuclear and cytoplasmic maturation of mouse oocytes after treatment with synthetic meiosis-activating sterol in vitro. BIOLOGY OF REPRODUCTION. 1999;61(5):1362-1372.
Hegele-Hartung, C., Kuhnke, J., Lessl, M., Grondahl, C., Ottesen, J., Beier, H. M., Eisner, S., et al. (1999). Nuclear and cytoplasmic maturation of mouse oocytes after treatment with synthetic meiosis-activating sterol in vitro. BIOLOGY OF REPRODUCTION, 61(5), 1362-1372. doi:10.1095/biolreprod61.5.1362
Hegele-Hartung, C., Kuhnke, J., Lessl, M., Grondahl, C., Ottesen, J., Beier, H. M., Eisner, S., and Eichenlaub-Ritter, U. (1999). Nuclear and cytoplasmic maturation of mouse oocytes after treatment with synthetic meiosis-activating sterol in vitro. BIOLOGY OF REPRODUCTION 61, 1362-1372.
Hegele-Hartung, C., et al., 1999. Nuclear and cytoplasmic maturation of mouse oocytes after treatment with synthetic meiosis-activating sterol in vitro. BIOLOGY OF REPRODUCTION, 61(5), p 1362-1372.
C. Hegele-Hartung, et al., “Nuclear and cytoplasmic maturation of mouse oocytes after treatment with synthetic meiosis-activating sterol in vitro”, BIOLOGY OF REPRODUCTION, vol. 61, 1999, pp. 1362-1372.
Hegele-Hartung, C., Kuhnke, J., Lessl, M., Grondahl, C., Ottesen, J., Beier, H.M., Eisner, S., Eichenlaub-Ritter, U.: Nuclear and cytoplasmic maturation of mouse oocytes after treatment with synthetic meiosis-activating sterol in vitro. BIOLOGY OF REPRODUCTION. 61, 1362-1372 (1999).
Hegele-Hartung, C, Kuhnke, J, Lessl, M, Grondahl, C, Ottesen, J, Beier, HM, Eisner, S, and Eichenlaub-Ritter, Ursula. “Nuclear and cytoplasmic maturation of mouse oocytes after treatment with synthetic meiosis-activating sterol in vitro”. BIOLOGY OF REPRODUCTION 61.5 (1999): 1362-1372.

34 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Role of meiosis activating sterols, MAS, in induced oocyte maturation.
Byskov AG, Andersen CY, Leonardsen L., Mol Cell Endocrinol 187(1-2), 2002
PMID: 11988327
Meiosis-activating sterol and the maturation of isolated mouse oocytes.
Downs SM, Ruan B, Schroepfer GJ., Biol Reprod 64(1), 2001
PMID: 11133661
Activation of meiotic maturation in rat oocytes after treatment with follicular fluid meiosis-activating sterol in vitro and ex vivo.
Hegele-Hartung C, Grützner M, Lessl M, Grøndahl C, Ottesen J, Brännström M., Biol Reprod 64(2), 2001
PMID: 11159342
The effects of meiosis activating sterol on in-vitro maturation and fertilization of human oocytes from stimulated and unstimulated ovaries.
Cavilla JL, Kennedy CR, Baltsen M, Klentzeris LD, Byskov AG, Hartshorne GM., Hum Reprod 16(3), 2001
PMID: 11228227

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