Nuclear and cytoplasmic maturation of mouse oocytes after treatment with synthetic meiosis-activating sterol in vitro

Hegele-Hartung C, Kuhnke J, Lessl M, Grondahl C, Ottesen J, Beier HM, Eisner S, Eichenlaub-Ritter U (1999)
BIOLOGY OF REPRODUCTION 61(5): 1362-1372.

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Synthetically produced meiosis-activating sterol, a sterol originally derived from follicular fluid (FF-MAS), induces meiotic maturation of mouse oocytes in vitro. We therefore compared FF-MAS-induced maturation of naked mouse oocytes arrested in prophase I by either hypoxanthine (Hx) or forskolin (Fo) with spontaneous maturation of naked oocytes. FF-MAS-treated oocytes overcame the meiotic block by Hx or Fo, although germinal vesicle breakdown was delayed by 11 h and 7 h, respectively. We also investigated the influence of FF-MAS on chromosome, microtubule, and ultrastructural dynamics in Hx-cultured oocytes by immunocytochemistry and electron microscopy. Similarly to spontaneously matured oocytes, chromosomes became aligned, a barrel-shaped spindle formed, and overall organelle distribution was normal in FF-MAS-matured oocytes. The number of small cytoplasmic asters was elevated in FF-MAS-treated oocytes. Although the number of cortical granules (CGs) was similar to that in spontaneously matured oocytes, the overall distance between CGs and oolemma was increased in the FF-MAS group. These observations suggest that the initiation of meiotic maturation in FF-MAS-treated oocytes in the presence of high cAMP levels leads to a delayed but otherwise normal nuclear maturation. FF-MAS appears to improve oocyte quality by supporting microtubule assembly and by delaying CG release, which is known to contribute to reduced fertilization.
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Hegele-Hartung C, Kuhnke J, Lessl M, et al. Nuclear and cytoplasmic maturation of mouse oocytes after treatment with synthetic meiosis-activating sterol in vitro. BIOLOGY OF REPRODUCTION. 1999;61(5):1362-1372.
Hegele-Hartung, C., Kuhnke, J., Lessl, M., Grondahl, C., Ottesen, J., Beier, H. M., Eisner, S., et al. (1999). Nuclear and cytoplasmic maturation of mouse oocytes after treatment with synthetic meiosis-activating sterol in vitro. BIOLOGY OF REPRODUCTION, 61(5), 1362-1372. doi:10.1095/biolreprod61.5.1362
Hegele-Hartung, C., Kuhnke, J., Lessl, M., Grondahl, C., Ottesen, J., Beier, H. M., Eisner, S., and Eichenlaub-Ritter, U. (1999). Nuclear and cytoplasmic maturation of mouse oocytes after treatment with synthetic meiosis-activating sterol in vitro. BIOLOGY OF REPRODUCTION 61, 1362-1372.
Hegele-Hartung, C., et al., 1999. Nuclear and cytoplasmic maturation of mouse oocytes after treatment with synthetic meiosis-activating sterol in vitro. BIOLOGY OF REPRODUCTION, 61(5), p 1362-1372.
C. Hegele-Hartung, et al., “Nuclear and cytoplasmic maturation of mouse oocytes after treatment with synthetic meiosis-activating sterol in vitro”, BIOLOGY OF REPRODUCTION, vol. 61, 1999, pp. 1362-1372.
Hegele-Hartung, C., Kuhnke, J., Lessl, M., Grondahl, C., Ottesen, J., Beier, H.M., Eisner, S., Eichenlaub-Ritter, U.: Nuclear and cytoplasmic maturation of mouse oocytes after treatment with synthetic meiosis-activating sterol in vitro. BIOLOGY OF REPRODUCTION. 61, 1362-1372 (1999).
Hegele-Hartung, C, Kuhnke, J, Lessl, M, Grondahl, C, Ottesen, J, Beier, HM, Eisner, S, and Eichenlaub-Ritter, Ursula. “Nuclear and cytoplasmic maturation of mouse oocytes after treatment with synthetic meiosis-activating sterol in vitro”. BIOLOGY OF REPRODUCTION 61.5 (1999): 1362-1372.
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34 Citations in Europe PMC

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Role of meiosis activating sterols, MAS, in induced oocyte maturation.
Byskov AG, Andersen CY, Leonardsen L., Mol Cell Endocrinol 187(1-2), 2002
PMID: 11988327
Meiosis-activating sterol and the maturation of isolated mouse oocytes.
Downs SM, Ruan B, Schroepfer GJ., Biol Reprod 64(1), 2001
PMID: 11133661
Activation of meiotic maturation in rat oocytes after treatment with follicular fluid meiosis-activating sterol in vitro and ex vivo.
Hegele-Hartung C, Grützner M, Lessl M, Grøndahl C, Ottesen J, Brännström M., Biol Reprod 64(2), 2001
PMID: 11159342
The effects of meiosis activating sterol on in-vitro maturation and fertilization of human oocytes from stimulated and unstimulated ovaries.
Cavilla JL, Kennedy CR, Baltsen M, Klentzeris LD, Byskov AG, Hartshorne GM., Hum Reprod 16(3), 2001
PMID: 11228227

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