The Corynebacterium glutamicum insertion sequence ISCg2 prefers conserved target sequences located adjacent to genes involved in aspartate and glutamate metabolism

Quast K, Bathe B, Pühler A, Kalinowski J (1999)
Mol Gen Genet 262(3): 568-578.

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Abstract
An IS element, termed ISCg2, was identified in the chromosome of Corynebacterium glutamicum ATCC 13032. After screening a cosmid library of the C. glutamicum ATCC 13032 genome, six copies of ISCg2 including their flanking regions were sequenced and analyzed. ISCg2 is 1636 bp in length and has 26-bp imperfect inverted repeats flanked by 3-bp direct repeats. By comparisons with other IS elements,ISCg2 was classified as a member of the IS30 family of insertion sequences. The six copies of ISCg2 were identical at the nucleotide level and were located in intergenic, AT-rich regions of the chromosome. The regions in which the six copies of ISCg2 were inserted displayed significant similarities. This similarity extends over a region of 65 bp, which was assumed to be the target region for ISCg2. Interestingly, live of the six copies of ISCg2 were located adjacent to genes that may be involved in aspartate and glutamate metabolism or its regulation. Investigation of the distribution of ISCg2 showed that the IS element is restricted to certain C. glutamicum strains. Analysis of various integration regions indicates active transposition of ISCg2. in C. glutamicum.
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Quast K, Bathe B, Pühler A, Kalinowski J. The Corynebacterium glutamicum insertion sequence ISCg2 prefers conserved target sequences located adjacent to genes involved in aspartate and glutamate metabolism. Mol Gen Genet. 1999;262(3):568-578.
Quast, K., Bathe, B., Pühler, A., & Kalinowski, J. (1999). The Corynebacterium glutamicum insertion sequence ISCg2 prefers conserved target sequences located adjacent to genes involved in aspartate and glutamate metabolism. Mol Gen Genet, 262(3), 568-578.
Quast, K., Bathe, B., Pühler, A., and Kalinowski, J. (1999). The Corynebacterium glutamicum insertion sequence ISCg2 prefers conserved target sequences located adjacent to genes involved in aspartate and glutamate metabolism. Mol Gen Genet 262, 568-578.
Quast, K., et al., 1999. The Corynebacterium glutamicum insertion sequence ISCg2 prefers conserved target sequences located adjacent to genes involved in aspartate and glutamate metabolism. Mol Gen Genet, 262(3), p 568-578.
K. Quast, et al., “The Corynebacterium glutamicum insertion sequence ISCg2 prefers conserved target sequences located adjacent to genes involved in aspartate and glutamate metabolism”, Mol Gen Genet, vol. 262, 1999, pp. 568-578.
Quast, K., Bathe, B., Pühler, A., Kalinowski, J.: The Corynebacterium glutamicum insertion sequence ISCg2 prefers conserved target sequences located adjacent to genes involved in aspartate and glutamate metabolism. Mol Gen Genet. 262, 568-578 (1999).
Quast, K, Bathe, B, Pühler, Alfred, and Kalinowski, Jörn. “The Corynebacterium glutamicum insertion sequence ISCg2 prefers conserved target sequences located adjacent to genes involved in aspartate and glutamate metabolism”. Mol Gen Genet 262.3 (1999): 568-578.
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8 Citations in Europe PMC

Data provided by Europe PubMed Central.

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PMID: 17367392
Manipulating corynebacteria, from individual genes to chromosomes.
Vertes AA, Inui M, Yukawa H., Appl. Environ. Microbiol. 71(12), 2005
PMID: 16332735
The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of L-aspartate-derived amino acids and vitamins.
Kalinowski J, Bathe B, Bartels D, Bischoff N, Bott M, Burkovski A, Dusch N, Eggeling L, Eikmanns BJ, Gaigalat L, Goesmann A, Hartmann M, Huthmacher K, Kramer R, Linke B, McHardy AC, Meyer F, Mockel B, Pfefferle W, Puhler A, Rey DA, Ruckert C, Rupp O, Sahm H, Wendisch VF, Wiegrabe I, Tauch A., J. Biotechnol. 104(1-3), 2003
PMID: 12948626
Strategy to sequence the genome of Corynebacterium glutamicum ATCC 13032: use of a cosmid and a bacterial artificial chromosome library.
Tauch A, Homann I, Mormann S, Ruberg S, Billault A, Bathe B, Brand S, Brockmann-Gretza O, Ruckert C, Schischka N, Wrenger C, Hoheisel J, Mockel B, Huthmacher K, Pfefferle W, Puhler A, Kalinowski J., J. Biotechnol. 95(1), 2002
PMID: 11879709

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