Steroid RU 486 inducible myogenesis by 10T1/2 fibroblastic mouse cells

Bartsch JW, Jackel M, Perz A, Jockusch H (2000)
FEBS letters 467(1): 123-127.

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For reconstruction or repair of damaged tissues, an artificially regulated switch from proliferation to differentiation would be of great advantage. To achieve conditional myogenesis, we expressed MyoD in mouse C3H 10T1/2 fibroblastic cells, using a gene regulation system based on the synthetic steroid RU 486, BS stable co-transfection of a plasmid construct with the RU 486 dependent activator and an integrating inducible MyoD construct, a cell clone, designated 10T-RM, was obtained in which MyoD expression was stringently controlled by RU 486, 12 h after addition of 10 nM RU 486 to 10T-RM cells, saturation levels of MyoD mRNA were observed and greater than or equal to 2 days later, mRNA for embryonal myosin heavy chain (MyHC(emb)) was abundant and mononucleated cells fused into myotubes. (C) 2000 Federation of European Biochemical Societies.
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Bartsch JW, Jackel M, Perz A, Jockusch H. Steroid RU 486 inducible myogenesis by 10T1/2 fibroblastic mouse cells. FEBS letters. 2000;467(1):123-127.
Bartsch, J. W., Jackel, M., Perz, A., & Jockusch, H. (2000). Steroid RU 486 inducible myogenesis by 10T1/2 fibroblastic mouse cells. FEBS letters, 467(1), 123-127.
Bartsch, J. W., Jackel, M., Perz, A., and Jockusch, H. (2000). Steroid RU 486 inducible myogenesis by 10T1/2 fibroblastic mouse cells. FEBS letters 467, 123-127.
Bartsch, J.W., et al., 2000. Steroid RU 486 inducible myogenesis by 10T1/2 fibroblastic mouse cells. FEBS letters, 467(1), p 123-127.
J.W. Bartsch, et al., “Steroid RU 486 inducible myogenesis by 10T1/2 fibroblastic mouse cells”, FEBS letters, vol. 467, 2000, pp. 123-127.
Bartsch, J.W., Jackel, M., Perz, A., Jockusch, H.: Steroid RU 486 inducible myogenesis by 10T1/2 fibroblastic mouse cells. FEBS letters. 467, 123-127 (2000).
Bartsch, JW, Jackel, M, Perz, A, and Jockusch, Harald. “Steroid RU 486 inducible myogenesis by 10T1/2 fibroblastic mouse cells”. FEBS letters 467.1 (2000): 123-127.
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