Surface-expressed E-cadherin, and mitochondrial and microtubule distribution in rescue of mouse embryos from 2-cell block by aggregation

Neganova IE, Sekirina GG, Eichenlaub-Ritter U (2000)
MOLECULAR HUMAN REPRODUCTION 6(5): 454-464.

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Zeitschriftenaufsatz | Veröffentlicht | Englisch
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Abstract / Bemerkung
E-cadherin (uvomorulin)-mediated cell interactions are essential for preimplantation development in mammals, We observed that E-cadherin is expressed at contact sites between blastomeres of 2-cell mouse embryos of non-blocking genotype (CBA x C57BL fl) explanted at 32 h post human chorionic gonadotrophin (HCG) and cultured in vitro, while blastomere rounding and reduced zones of contact and E-cadherin-staining were observed in embryos of a blocking strain (MF1) arrested at the 2-cell stage, Embryos of MF1 strain can be rescued by aggregation with four 2-cell embryos of the non-blocking genotype, An early event in rescue is E-cadherin expression at contact zones between adjacent embryos of different genotype in aggregation chimeras. E-cadherin-mediated signalling appears important for the rescue (including formation of adherens-like contacts, cell polarization and morphogenetic processes) since there is no rescue when E-cadherin-specific antibodies are present during phytohaemagglutinin-mediated aggregation and subsequent culture. In blocked embryos, the distribution of microtubules is disturbed and concomitantly mitochondria cluster around the nucleus. Rescue by aggregation retains normal mitochondrial distribution in the presence of a dense microtubular lattice in all blastomeres, Therefore, E-cadherin-mediated signalling and its downstream effects on cytoskeletal organization are essential in the rescue of blocking embryos by aggregation. Normal preimplantation development appears to be dependent on the polarized expression of surface E-cadherin and the microtubule-mediated dispersal of mitochondria.
Erscheinungsjahr
Zeitschriftentitel
MOLECULAR HUMAN REPRODUCTION
Band
6
Zeitschriftennummer
5
Seite
454-464
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Neganova IE, Sekirina GG, Eichenlaub-Ritter U. Surface-expressed E-cadherin, and mitochondrial and microtubule distribution in rescue of mouse embryos from 2-cell block by aggregation. MOLECULAR HUMAN REPRODUCTION. 2000;6(5):454-464.
Neganova, I. E., Sekirina, G. G., & Eichenlaub-Ritter, U. (2000). Surface-expressed E-cadherin, and mitochondrial and microtubule distribution in rescue of mouse embryos from 2-cell block by aggregation. MOLECULAR HUMAN REPRODUCTION, 6(5), 454-464. doi:10.1093/molehr/6.5.454
Neganova, I. E., Sekirina, G. G., and Eichenlaub-Ritter, U. (2000). Surface-expressed E-cadherin, and mitochondrial and microtubule distribution in rescue of mouse embryos from 2-cell block by aggregation. MOLECULAR HUMAN REPRODUCTION 6, 454-464.
Neganova, I.E., Sekirina, G.G., & Eichenlaub-Ritter, U., 2000. Surface-expressed E-cadherin, and mitochondrial and microtubule distribution in rescue of mouse embryos from 2-cell block by aggregation. MOLECULAR HUMAN REPRODUCTION, 6(5), p 454-464.
I.E. Neganova, G.G. Sekirina, and U. Eichenlaub-Ritter, “Surface-expressed E-cadherin, and mitochondrial and microtubule distribution in rescue of mouse embryos from 2-cell block by aggregation”, MOLECULAR HUMAN REPRODUCTION, vol. 6, 2000, pp. 454-464.
Neganova, I.E., Sekirina, G.G., Eichenlaub-Ritter, U.: Surface-expressed E-cadherin, and mitochondrial and microtubule distribution in rescue of mouse embryos from 2-cell block by aggregation. MOLECULAR HUMAN REPRODUCTION. 6, 454-464 (2000).
Neganova, IE, Sekirina, GG, and Eichenlaub-Ritter, Ursula. “Surface-expressed E-cadherin, and mitochondrial and microtubule distribution in rescue of mouse embryos from 2-cell block by aggregation”. MOLECULAR HUMAN REPRODUCTION 6.5 (2000): 454-464.

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Embryo Aggregation Promotes Derivation Efficiency of Outgrowths from Porcine Blastocysts.
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Fujimoto VY, Browne RW, Bloom MS, Sakkas D, Alikani M., Fertil Steril 95(4), 2011
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Zhou W, Xiang T, Walker S, Abruzzese RV, Hwang E, Farrar V, Findeisen B, Sadeghieh S, Arenivas F, Chen SH, Polejaeva I., Mol Reprod Dev 75(8), 2008
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In vitro development and cell allocation of porcine blastocysts derived by aggregation of in vitro fertilized embryos.
Lee SG, Park CH, Choi DH, Kim HS, Ka HH, Lee CK., Mol Reprod Dev 74(11), 2007
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Effect of female age on mouse oocyte developmental competence following mitochondrial injury.
Thouas GA, Trounson AO, Jones GM., Biol Reprod 73(2), 2005
PMID: 15843494
Vezatin, a protein associated to adherens junctions, is required for mouse blastocyst morphogenesis.
Hyenne V, Louvet-Vallée S, El-Amraoui A, Petit C, Maro B, Simmler MC., Dev Biol 287(1), 2005
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Interpretation of reprogramming to predict the success of somatic cell cloning.
Eckardt S, McLaughlin KJ., Anim Reprod Sci 82-83(), 2004
PMID: 15271446
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