Phosphate uptake across the tonoplast of intact vacuoles isolated from suspension-cultured cells of Catharanthus roseus (L.) G. Don

Massonneau A, Martinoia E, Dietz K-J, Mimura T (2000)
PLANTA 211(3): 390-395.

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Abstract
Transport of inorganic orthophosphate (Pi) across the tonoplast membrane was studied using intact vacuoles isolated from suspension-cultured cells of Catharanthus roseus. Orthophosphate uptake was strongly stimulated in the presence of Mg-ATP and Mg-pyrophosphate and inhibited by bafilomycin and concanamycin which are potent inhibitors of the vacuolar H+-ATPase. These results indicated that the buildup of an electrochemical gradient by the H+ pumps tvas essential for the uptake of Pi. Potassium thiocyanate, which dissipates the membrane potential across the tonoplast, strongly inhibited the Mg-ATP-stimulated uptake of Pi, while only a weak inhibition was observed in the presence of NH4Cl, which dissipates the pH gl-adient. These results indicate that, as observed for other anions like malate or chloride, the electrical component is the driving force of Pi uptake, whereas the Delta pH plays only a minor role. Possible competitive inhibitors of Pi, MoO42-, VO43- and CrO42- were tested. Among them, CrO42- strongly inhibited Pi uptake into the vacuoles. Various inhibitors of anion transport were also tested. Only 4,4-diisothiocyanostilbene-2,2'-disulfonic acid strongly inhibited Pi uptake into the vacuoles. The function of the vacuolar Pi transporters for cytoplasmic Pi homeostasis is discussed.
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Massonneau A, Martinoia E, Dietz K-J, Mimura T. Phosphate uptake across the tonoplast of intact vacuoles isolated from suspension-cultured cells of Catharanthus roseus (L.) G. Don. PLANTA. 2000;211(3):390-395.
Massonneau, A., Martinoia, E., Dietz, K. - J., & Mimura, T. (2000). Phosphate uptake across the tonoplast of intact vacuoles isolated from suspension-cultured cells of Catharanthus roseus (L.) G. Don. PLANTA, 211(3), 390-395.
Massonneau, A., Martinoia, E., Dietz, K. - J., and Mimura, T. (2000). Phosphate uptake across the tonoplast of intact vacuoles isolated from suspension-cultured cells of Catharanthus roseus (L.) G. Don. PLANTA 211, 390-395.
Massonneau, A., et al., 2000. Phosphate uptake across the tonoplast of intact vacuoles isolated from suspension-cultured cells of Catharanthus roseus (L.) G. Don. PLANTA, 211(3), p 390-395.
A. Massonneau, et al., “Phosphate uptake across the tonoplast of intact vacuoles isolated from suspension-cultured cells of Catharanthus roseus (L.) G. Don”, PLANTA, vol. 211, 2000, pp. 390-395.
Massonneau, A., Martinoia, E., Dietz, K.-J., Mimura, T.: Phosphate uptake across the tonoplast of intact vacuoles isolated from suspension-cultured cells of Catharanthus roseus (L.) G. Don. PLANTA. 211, 390-395 (2000).
Massonneau, A, Martinoia, E, Dietz, Karl-Josef, and Mimura, T. “Phosphate uptake across the tonoplast of intact vacuoles isolated from suspension-cultured cells of Catharanthus roseus (L.) G. Don”. PLANTA 211.3 (2000): 390-395.
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