Expression of human membrane type 1 matrix metalloproteinase in Pichia pastoris

Roderfeld M, Buttner FH, Bartnik E, Tschesche H (2000)
PROTEIN EXPRESSION AND PURIFICATION 19(3): 369-374.

Download
Es wurde kein Volltext hochgeladen. Nur Publikationsnachweis!
Zeitschriftenaufsatz | Veröffentlicht | Englisch
Autor
; ; ;
Abstract / Bemerkung
A soluble, C-terminal truncated form of human membrane type 1 matrix metalloproteinase (MT1-MMP) containing the hemopexin-like domain was expressed in Pichia pastoris strain KM71. High levels of secreted protein were detected. Although the c-DNA for the proenzyme (Ala(21)-Glu(523) called Delta TM-MT1-MMP) was cloned, almost only active MT1-MMP (Tyr(112)-GlU(523)) with identical N-terminus as described for the wildtype enzyme was isolated. This active enzyme was highly purified and characterized with respect to its biochemical properties, The recombinant protein showed high stability against autolysis and proteolysis by yeast proteases, although the calculated in vivo half-life is rather low. The biochemical properties of this new MT1-MMP species were compared with the well-characterized catalytic domain (Ile(114)-Ile(318)) of MT1-MMP, The novel form of MT1-MMP exhibited a higher stability against autolysis than the isolated catalytic domain (Ile(114)-Ile(318)). (C) 2000AcademicPress.
Erscheinungsjahr
Zeitschriftentitel
PROTEIN EXPRESSION AND PURIFICATION
Band
19
Zeitschriftennummer
3
Seite
369-374
ISSN
PUB-ID

Zitieren

Roderfeld M, Buttner FH, Bartnik E, Tschesche H. Expression of human membrane type 1 matrix metalloproteinase in Pichia pastoris. PROTEIN EXPRESSION AND PURIFICATION. 2000;19(3):369-374.
Roderfeld, M., Buttner, F. H., Bartnik, E., & Tschesche, H. (2000). Expression of human membrane type 1 matrix metalloproteinase in Pichia pastoris. PROTEIN EXPRESSION AND PURIFICATION, 19(3), 369-374. doi:10.1006/prep.2000.1259
Roderfeld, M., Buttner, F. H., Bartnik, E., and Tschesche, H. (2000). Expression of human membrane type 1 matrix metalloproteinase in Pichia pastoris. PROTEIN EXPRESSION AND PURIFICATION 19, 369-374.
Roderfeld, M., et al., 2000. Expression of human membrane type 1 matrix metalloproteinase in Pichia pastoris. PROTEIN EXPRESSION AND PURIFICATION, 19(3), p 369-374.
M. Roderfeld, et al., “Expression of human membrane type 1 matrix metalloproteinase in Pichia pastoris”, PROTEIN EXPRESSION AND PURIFICATION, vol. 19, 2000, pp. 369-374.
Roderfeld, M., Buttner, F.H., Bartnik, E., Tschesche, H.: Expression of human membrane type 1 matrix metalloproteinase in Pichia pastoris. PROTEIN EXPRESSION AND PURIFICATION. 19, 369-374 (2000).
Roderfeld, M, Buttner, FH, Bartnik, E, and Tschesche, Harald. “Expression of human membrane type 1 matrix metalloproteinase in Pichia pastoris”. PROTEIN EXPRESSION AND PURIFICATION 19.3 (2000): 369-374.

4 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Latent MMP-9 is bound to TIMP-1 before secretion.
Roderfeld M, Graf J, Giese B, Salguero-Palacios R, Tschuschner A, Müller-Newen G, Roeb E., Biol Chem 388(11), 2007
PMID: 17976016
Catalytic- and ecto-domains of membrane type 1-matrix metalloproteinase have similar inhibition profiles but distinct endopeptidase activities.
Hurst DR, Schwartz MA, Ghaffari MA, Jin Y, Tschesche H, Fields GB, Sang QX., Biochem J 377(pt 3), 2004
PMID: 14533979

Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®

Quellen

PMID: 10910727
PubMed | Europe PMC

Suchen in

Google Scholar