The promoter of the Vicia faba L. gene VfEnod12 encoding an early nodulin is active in cortical cells and nodule primordia of transgenic hairy roots of Vicia hirsuta as well as in the prefixing zone II of mature transgenic V-hirsuta root nodules

Fruhling M, Schroder G, Hohnjec N, Pühler A, Perlick AM, Küster H (2000)
PLANT SCIENCE 160(1): 67-75.

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Abstract
A full-length cDNA encoding the Vicia faba L. early nodulin VfEnod12 was isolated. The deduced protein sequence specified a 90 amino acid protein with a MW of 10 206 and contained a putative signal peptide sequence followed by PPX3 repeats characteristic of Enod12 proteins. The VfEnod12 gene was found to be expressed specifically in root nodules as early as 3 days post inoculation with Rhizobium leguminosarum bv. viciae. In mature nodules, VfEnod12 transcripts were confined to the prefixing zone II. A 3.3 kb genomic fragment carrying the complete VfEnod12 coding region was isolated. No intervening sequences were identified in the coding region. A promoter fragment carrying the -692/-41 region mediated reporter gene expression in root cortical cells, module primordia and the prefixing zone II of transgenic Vicia hirsuta root nodules. This fragment contained a putative binding site for the transcription factor ENBP1. In contrast to the highly conserved terminal AATAA motif of the ENBP1 binding site of known Enod12 promoters, the VfEnod12 promoter was characterized by an altered terminal AATAT sequence. This alteration did not interfere with VfEnod12 promoter activity in transgenic roots and nodules of V. hirsuta. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.
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Fruhling M, Schroder G, Hohnjec N, Pühler A, Perlick AM, Küster H. The promoter of the Vicia faba L. gene VfEnod12 encoding an early nodulin is active in cortical cells and nodule primordia of transgenic hairy roots of Vicia hirsuta as well as in the prefixing zone II of mature transgenic V-hirsuta root nodules. PLANT SCIENCE. 2000;160(1):67-75.
Fruhling, M., Schroder, G., Hohnjec, N., Pühler, A., Perlick, A. M., & Küster, H. (2000). The promoter of the Vicia faba L. gene VfEnod12 encoding an early nodulin is active in cortical cells and nodule primordia of transgenic hairy roots of Vicia hirsuta as well as in the prefixing zone II of mature transgenic V-hirsuta root nodules. PLANT SCIENCE, 160(1), 67-75.
Fruhling, M., Schroder, G., Hohnjec, N., Pühler, A., Perlick, A. M., and Küster, H. (2000). The promoter of the Vicia faba L. gene VfEnod12 encoding an early nodulin is active in cortical cells and nodule primordia of transgenic hairy roots of Vicia hirsuta as well as in the prefixing zone II of mature transgenic V-hirsuta root nodules. PLANT SCIENCE 160, 67-75.
Fruhling, M., et al., 2000. The promoter of the Vicia faba L. gene VfEnod12 encoding an early nodulin is active in cortical cells and nodule primordia of transgenic hairy roots of Vicia hirsuta as well as in the prefixing zone II of mature transgenic V-hirsuta root nodules. PLANT SCIENCE, 160(1), p 67-75.
M. Fruhling, et al., “The promoter of the Vicia faba L. gene VfEnod12 encoding an early nodulin is active in cortical cells and nodule primordia of transgenic hairy roots of Vicia hirsuta as well as in the prefixing zone II of mature transgenic V-hirsuta root nodules”, PLANT SCIENCE, vol. 160, 2000, pp. 67-75.
Fruhling, M., Schroder, G., Hohnjec, N., Pühler, A., Perlick, A.M., Küster, H.: The promoter of the Vicia faba L. gene VfEnod12 encoding an early nodulin is active in cortical cells and nodule primordia of transgenic hairy roots of Vicia hirsuta as well as in the prefixing zone II of mature transgenic V-hirsuta root nodules. PLANT SCIENCE. 160, 67-75 (2000).
Fruhling, M, Schroder, G, Hohnjec, N, Pühler, Alfred, Perlick, AM, and Küster, Helge. “The promoter of the Vicia faba L. gene VfEnod12 encoding an early nodulin is active in cortical cells and nodule primordia of transgenic hairy roots of Vicia hirsuta as well as in the prefixing zone II of mature transgenic V-hirsuta root nodules”. PLANT SCIENCE 160.1 (2000): 67-75.
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46 References

Data provided by Europe PubMed Central.

Rhizobium meliloti elicits transient expression of the early nodulin gene ENOD12 in the differentiating root epidermis of transgenic alfalfa.
Pichon M, Journet EP, Dedieu A, de Billy F, Truchet G, Barker DG., Plant Cell 4(10), 1992
PMID: 1446169
ENOD12 gene expression as a molecular marker for comparing Rhizobium-dependent and -independent nodulation in alfalfa
Pichon, Mol. Plant-Microbe Interact. 7(), 1994
Transgenic root nodules of Vicia hirsuta: A fast and efficient system for the study of gene expression in indeterminate-type nodules
Quandt, Mol. Plant-Microbe Interact. 6(), 1993

Sambrook, 1989
The ENOD12 gene product is involved in the infection process during the pea-Rhizobium interaction.
Scheres B, Van De Wiel C, Zalensky A, Horvath B, Spaink H, Van Eck H, Zwartkruis F, Wolters AM, Gloudemans T, Van Kammen A., Cell 60(2), 1990
PMID: 2297789
Sequential induction of nodulin gene expression in the developing pea nodule.
Scheres B, van Engelen F, van der Knaap E, van de Wiel C, van Kammen A, Bisseling T., Plant Cell 2(8), 1990
PMID: 2152123
What makes nodulation signals host-plant specific?
Schultze M, Kondorosi A., Trends Microbiol. 3(10), 1995
PMID: 8564354
Structure and function of plant cell wall proteins.
Showalter AM., Plant Cell 5(1), 1993
PMID: 8439747
A broad host range mobilization system for in vivo genetic engineering: Transposon mutagenesis in gram negative bacteria
Simon, Biotechnology 1(), 1983
The current status and portability of our sequence handling software
Staden, Nucl. Acids Res. 14(), 1986
Suggested nomenclature for plant genes involved in nodulation and symbiosis
van, Plant Mol. Biol. Rep. 2(), 1984
Root nodule development: origin, function and regulation of nodulin genes
Verma, Phys. Plantarum 85(), 1992
VsENOD5, VsENOD12 and VsENOD40 expression during Rhizobium-induced nodule formation on Vicia sativa roots.
Vijn I, Yang WC, Pallisgard N, Ostergaard Jensen E, van Kammen A, Bisseling T., Plant Mol. Biol. 28(6), 1995
PMID: 7548828
A 200 bp region of the pea ENOD12 promoter is sufficient for nodule-specific and nod factor induced expression.
Vijn I, Christiansen H, Lauridsen P, Kardailsky I, Quandt HJ, Broer I, Drenth J, Ostergaard Jensen E, van Kammen A, Bisseling T., Plant Mol. Biol. 28(6), 1995
PMID: 7548827
Topogenic signals in integral membrane proteins
von, Eur. J. Biochem. 174(), 1992
A simplified protocol for fast plasmid DNA sequencing
Zimmermann, Nucl. Acids Res. 18(), 1990

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