Effects of low O-2 and ageing on spindles and chromosomes in mouse oocytes from pre-antral follicle culture

Hu Y, Betzendahl I, Cortvrindt R, Smitz J, Eichenlaub-Ritter U (2001)
HUMAN REPRODUCTION 16(4): 737-748.

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To assess their quality, spindles were analysed in mouse oocytes from pre-antral follicle culture. High or low oxygen tension was present during the last 16 or 20 h post human chorionic gonadotrophin (HCG)/epidermal growth factor (EGF) addition. Most oocytes from pre-antral follicle culture possessed typical anastral spindles with flat poles resembling those of ovulated, in-vivo-matured oocytes of sexually mature mice, while denuded oocytes in-vitro matured to metaphase II (MII) formed significantly longer, slender spindles with pointed, narrow poles. Spindles in oocytes from follicle culture were only slightly shorter and less compact at the equator as compared with those of oocytes matured in vivo. Chromosomes were well aligned at the equator in MII oocytes obtained from follicle culture with high oxygen. Maturation rate was significantly reduced by lowering oxygen tension to 5% O-2. Prolonged culture and the presence of only 5% O-2 dramatically increased the percentage of MII oocytes with unaligned chromosomes. These observations indicate that sufficient oxygen supply and time of retrieval after initiation of resumption of maturation by HCG as well as the microenvironment and cell-cell interactions between oocytes and their somatic compartment are critical in affecting the oocyte's capacity to mature to MII, to form a functional spindle, and to align chromosomes correctly.
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Hu Y, Betzendahl I, Cortvrindt R, Smitz J, Eichenlaub-Ritter U. Effects of low O-2 and ageing on spindles and chromosomes in mouse oocytes from pre-antral follicle culture. HUMAN REPRODUCTION. 2001;16(4):737-748.
Hu, Y., Betzendahl, I., Cortvrindt, R., Smitz, J., & Eichenlaub-Ritter, U. (2001). Effects of low O-2 and ageing on spindles and chromosomes in mouse oocytes from pre-antral follicle culture. HUMAN REPRODUCTION, 16(4), 737-748. doi:10.1093/humrep/16.4.737
Hu, Y., Betzendahl, I., Cortvrindt, R., Smitz, J., and Eichenlaub-Ritter, U. (2001). Effects of low O-2 and ageing on spindles and chromosomes in mouse oocytes from pre-antral follicle culture. HUMAN REPRODUCTION 16, 737-748.
Hu, Y., et al., 2001. Effects of low O-2 and ageing on spindles and chromosomes in mouse oocytes from pre-antral follicle culture. HUMAN REPRODUCTION, 16(4), p 737-748.
Y. Hu, et al., “Effects of low O-2 and ageing on spindles and chromosomes in mouse oocytes from pre-antral follicle culture”, HUMAN REPRODUCTION, vol. 16, 2001, pp. 737-748.
Hu, Y., Betzendahl, I., Cortvrindt, R., Smitz, J., Eichenlaub-Ritter, U.: Effects of low O-2 and ageing on spindles and chromosomes in mouse oocytes from pre-antral follicle culture. HUMAN REPRODUCTION. 16, 737-748 (2001).
Hu, Y, Betzendahl, I, Cortvrindt, R, Smitz, J, and Eichenlaub-Ritter, Ursula. “Effects of low O-2 and ageing on spindles and chromosomes in mouse oocytes from pre-antral follicle culture”. HUMAN REPRODUCTION 16.4 (2001): 737-748.
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Natriuretic peptides improve the developmental competence of in vitro cultured porcine oocytes.
Zhang Y, Wang H, Liu W, Yang Y, Wang X, Zhang Z, Guo Q, Wang C, Xia G., Reprod. Biol. Endocrinol. 15(1), 2017
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Relationship between pre-ICSI meiotic spindle angle, ovarian reserve, gonadotropin stimulation, and pregnancy outcomes.
Mahfoudh AM, Moon JH, Henderson S, Garcia-Cerrudo E, Son WY, Dahan MH., J. Assist. Reprod. Genet. 34(5), 2017
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Peritoneal Fluid From Infertile Women With Minimal/Mild Endometriosis Compromises the Meiotic Spindle of Metaphase II Bovine Oocytes: A Pilot Study.
Jianini BTGM, Giorgi VSI, Da Broi MG, de Paz CCP, Rosa E Silva JC, Ferriani RA, Navarro PA., Reprod Sci 24(9), 2017
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Ovine secondary follicles vitrified out the ovarian tissue grow and develop in vitro better than those vitrified into the ovarian fragments.
Lunardi FO, de Aguiar FL, Duarte AB, Araujo VR, de Lima LF, Ribeiro de Sa NA, Vieira Correia HH, Domingues SF, Campello CC, Smitz J, de Figueiredo JR, Ribeiro Rodrigues AP., Theriogenology 85(7), 2016
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Liu S, Jiang L, Zhong T, Kong S, Zheng R, Kong F, Zhang C, Zhang L, An L., PLoS ONE 10(8), 2015
PMID: 26275143
Regulation of PGC-1α Isoform Expression in Skeletal Muscles.
Popov DV, Lysenko EA, Kuzmin IV, Vinogradova V, Grigoriev AI., Acta Naturae 7(1), 2015
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Spanel-Borowski K, Nowicki M, Borlak J, Trapphoff T, Eichenlaub-Ritter U., Cells Tissues Organs (Print) 197(1), 2013
PMID: 22965003
Meiosis in a bottle: new approaches to overcome Mammalian meiocyte study limitations.
Roig I, Brieno-Enriquez MA, Caldes MG., Genes (Basel) 2(1), 2011
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Spindle and chromosome configurations of in vitro-matured oocytes from polycystic ovary syndrome and ovulatory infertile women: a pilot study.
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PMID: 20821044
[State of the art on in vitro folliculogenesis in mouse].
Mazoyer C, Courbiere B, Salle B, Smitz J, Lornage J., Gynecol Obstet Fertil 36(1), 2008
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PMID: 18065258

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