Proteome analysis of Corynebacterium glutamicum

Hermann T, Pfefferle W, Baumann C, Busker E, Schaffer S, Bott M, Sahm H, Dusch N, Kalinowski J, Pühler A, Bendt AK, et al. (2001)
ELECTROPHORESIS 22(9): 1712-1723.

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Zeitschriftenaufsatz | Veröffentlicht | Englisch
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Abstract / Bemerkung
By the use of different Corynebacterium glutamicum strains move than 1.4 million tons of amino acids, mainly L-glutamate and L-lysine, are produced per year. A project was started recently to elucidate the complete DNA sequence of this bacterium. In this communication we describe an approach to analyze the C, glutamicum proteome, based on this genetic information, by a combination of two-dimensional (2-D) gel electrophoresis and protein identification via microsequencing or mass spectrometry. We used these techniques to resolve proteins of C, glutamicum with the aim to establish 2-D protein maps as a tool for basic microbiology and for strain improvement. In order to analyze the C. glutamicum proteome, methods were established to fractionate the C. glutamicum proteins according to functional entities, i.e., cytoplasm, membranes, and cell wall. Protein spots of the cytoplasmic and membrane fraction were identified by N-terminal sequencing, immunodetection, matrix assisted laser desorption/ionization-time of flight-mass spectrometry (MALDI-TOF-MS) and electrospray ionization-mass spectrometry (ESI-MS). Additionally, a protocol to analyze proteins secreted by C. glutamicum was established. Approximately 40 protein spots were observed on silver-stained 2-D gels, 12 of which were identified.
Erscheinungsjahr
Zeitschriftentitel
ELECTROPHORESIS
Band
22
Zeitschriftennummer
9
Seite
1712-1723
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Hermann T, Pfefferle W, Baumann C, et al. Proteome analysis of Corynebacterium glutamicum. ELECTROPHORESIS. 2001;22(9):1712-1723.
Hermann, T., Pfefferle, W., Baumann, C., Busker, E., Schaffer, S., Bott, M., Sahm, H., et al. (2001). Proteome analysis of Corynebacterium glutamicum. ELECTROPHORESIS, 22(9), 1712-1723. doi:10.1002/1522-2683(200105)22:9<1712::AID-ELPS1712>3.0.CO;2-G
Hermann, T., Pfefferle, W., Baumann, C., Busker, E., Schaffer, S., Bott, M., Sahm, H., Dusch, N., Kalinowski, J., Pühler, A., et al. (2001). Proteome analysis of Corynebacterium glutamicum. ELECTROPHORESIS 22, 1712-1723.
Hermann, T., et al., 2001. Proteome analysis of Corynebacterium glutamicum. ELECTROPHORESIS, 22(9), p 1712-1723.
T. Hermann, et al., “Proteome analysis of Corynebacterium glutamicum”, ELECTROPHORESIS, vol. 22, 2001, pp. 1712-1723.
Hermann, T., Pfefferle, W., Baumann, C., Busker, E., Schaffer, S., Bott, M., Sahm, H., Dusch, N., Kalinowski, J., Pühler, A., Bendt, A.K., Kramer, R., Burkovski, A.: Proteome analysis of Corynebacterium glutamicum. ELECTROPHORESIS. 22, 1712-1723 (2001).
Hermann, T., Pfefferle, W., Baumann, C., Busker, E., Schaffer, S., Bott, M., Sahm, H., Dusch, N., Kalinowski, Jörn, Pühler, Alfred, Bendt, A. K., Kramer, R., and Burkovski, A. “Proteome analysis of Corynebacterium glutamicum”. ELECTROPHORESIS 22.9 (2001): 1712-1723.

51 Zitationen in Europe PMC

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A high-resolution reference map for cytoplasmic and membrane-associated proteins of Corynebacterium glutamicum.
Schaffer S, Weil B, Nguyen VD, Dongmann G, Günther K, Nickolaus M, Hermann T, Bott M., Electrophoresis 22(20), 2001
PMID: 11824608

30 References

Daten bereitgestellt von Europe PubMed Central.

Two-dimensional electrophoresis of membrane proteins: a current challenge for immobilized pH gradients.
Adessi C, Miege C, Albrieux C, Rabilloud T., Electrophoresis 18(1), 1997
PMID: 9059834
Low molecular weight proteins: a challenge for post-genomic research.
Rudd KE, Humphery-Smith I, Wasinger VC, Bairoch A., Electrophoresis 19(4), 1998
PMID: 9588799
Two-dimensional gel electrophoresis for proteome projects: the effects of protein hydrophobicity and copy number.
Wilkins MR, Gasteiger E, Sanchez JC, Bairoch A, Hochstrasser DF., Electrophoresis 19(8-9), 1998
PMID: 9694302

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