Relaxed rrn expression and amino acid requirement of a Corynebacterium glutamicum rel mutant defective in (p)ppGpp metabolism

Tauch A, Wehmeier L, Gotker S, Pühler A, Kalinowski J (2001)
FEMS Microbiol Lett 201(1): 53-58.

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Abstract
The stringent response in Corynebacterium glutamicum was investigated. Sets of rrn-cat fusions were constructed in their native chromosomal position to examine the effects of amino acid starvation in a rel(-) strain and a Delta rel mutant defective in (p)ppGpp metabolism. The expression of the six rm operons in the rel(+) control was stringently regulated and reduced to 79% upon induction of amino acid starvation. The Delta rel mutant displayed a relaxed regulation and was unable to reduce the rrn expression under amino acid depletion conditions. In addition, the Delta rel mutant grew more slowly in minimal medium than a rel(+) control. This growth effect was restored by a plasmid-encoded copy. of rel or, alternatively, by supplementation of the minimal medium with the amino acid mixture casamino acids. In particular, the Delta rel strain of C. glutamicum displayed a requirement For the amino acids histidine and serine. (C) 2001 Published by Elsevier Science B.V. on behalf of the Federation of European Microbiological Societies.
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Tauch A, Wehmeier L, Gotker S, Pühler A, Kalinowski J. Relaxed rrn expression and amino acid requirement of a Corynebacterium glutamicum rel mutant defective in (p)ppGpp metabolism. FEMS Microbiol Lett. 2001;201(1):53-58.
Tauch, A., Wehmeier, L., Gotker, S., Pühler, A., & Kalinowski, J. (2001). Relaxed rrn expression and amino acid requirement of a Corynebacterium glutamicum rel mutant defective in (p)ppGpp metabolism. FEMS Microbiol Lett, 201(1), 53-58.
Tauch, A., Wehmeier, L., Gotker, S., Pühler, A., and Kalinowski, J. (2001). Relaxed rrn expression and amino acid requirement of a Corynebacterium glutamicum rel mutant defective in (p)ppGpp metabolism. FEMS Microbiol Lett 201, 53-58.
Tauch, A., et al., 2001. Relaxed rrn expression and amino acid requirement of a Corynebacterium glutamicum rel mutant defective in (p)ppGpp metabolism. FEMS Microbiol Lett, 201(1), p 53-58.
A. Tauch, et al., “Relaxed rrn expression and amino acid requirement of a Corynebacterium glutamicum rel mutant defective in (p)ppGpp metabolism”, FEMS Microbiol Lett, vol. 201, 2001, pp. 53-58.
Tauch, A., Wehmeier, L., Gotker, S., Pühler, A., Kalinowski, J.: Relaxed rrn expression and amino acid requirement of a Corynebacterium glutamicum rel mutant defective in (p)ppGpp metabolism. FEMS Microbiol Lett. 201, 53-58 (2001).
Tauch, A, Wehmeier, L, Gotker, S, Pühler, Alfred, and Kalinowski, Jörn. “Relaxed rrn expression and amino acid requirement of a Corynebacterium glutamicum rel mutant defective in (p)ppGpp metabolism”. FEMS Microbiol Lett 201.1 (2001): 53-58.
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