Characterization of C-terminally truncated human tissue inhibitor of metalloproteinases-4 expressed in Pichia pastoris

Stratmann B, Farr M, Tschesche H (2001)
BIOLOGICAL CHEMISTRY 382(6): 987-991.

Journal Article | Published | English

No fulltext has been uploaded

Author
; ;
Abstract
The tight regulation of extracellular matrix remodeling and degradation is of great importance in physiological processes like development and morphogenesis, as well as in pathological situations like tumor invasion and metastasis. Tissue inhibitors of metalloproteinases (TIMPs) are the naturally occuring inhibitors of matrix metalloproteinases, which are involved in matrix turnover. In this report we describe the cloning of human TIMP-4 from a human adenocarcinoma and an osteosarcoma cell line and the expression of the inhibitory domain in the methylotrophic yeast Pichia pastoris. The inhibition of MMP-8, -9, -12, -13 and -14 by the N-terminal domain of TIMP-4 was analysed. Using a fluorescent MCA-peptide, K-i values for each subclass of MMPs were determined. With dissociation constants in the nanomolar range, TIMP-4 seems to be a good inhibitor for all classes of MMPs without remarkable preference for special MMPs.
Publishing Year
ISSN
PUB-ID

Cite this

Stratmann B, Farr M, Tschesche H. Characterization of C-terminally truncated human tissue inhibitor of metalloproteinases-4 expressed in Pichia pastoris. BIOLOGICAL CHEMISTRY. 2001;382(6):987-991.
Stratmann, B., Farr, M., & Tschesche, H. (2001). Characterization of C-terminally truncated human tissue inhibitor of metalloproteinases-4 expressed in Pichia pastoris. BIOLOGICAL CHEMISTRY, 382(6), 987-991.
Stratmann, B., Farr, M., and Tschesche, H. (2001). Characterization of C-terminally truncated human tissue inhibitor of metalloproteinases-4 expressed in Pichia pastoris. BIOLOGICAL CHEMISTRY 382, 987-991.
Stratmann, B., Farr, M., & Tschesche, H., 2001. Characterization of C-terminally truncated human tissue inhibitor of metalloproteinases-4 expressed in Pichia pastoris. BIOLOGICAL CHEMISTRY, 382(6), p 987-991.
B. Stratmann, M. Farr, and H. Tschesche, “Characterization of C-terminally truncated human tissue inhibitor of metalloproteinases-4 expressed in Pichia pastoris”, BIOLOGICAL CHEMISTRY, vol. 382, 2001, pp. 987-991.
Stratmann, B., Farr, M., Tschesche, H.: Characterization of C-terminally truncated human tissue inhibitor of metalloproteinases-4 expressed in Pichia pastoris. BIOLOGICAL CHEMISTRY. 382, 987-991 (2001).
Stratmann, B, Farr, M, and Tschesche, Harald. “Characterization of C-terminally truncated human tissue inhibitor of metalloproteinases-4 expressed in Pichia pastoris”. BIOLOGICAL CHEMISTRY 382.6 (2001): 987-991.
This data publication is cited in the following publications:
This publication cites the following data publications:

7 Citations in Europe PMC

Data provided by Europe PubMed Central.

Tissue inhibitor of matrix metalloproteinases 4 (TIMP4) in a population of young adults: relations to cardiovascular risk markers and carotid artery intima-media thickness. The Cardiovascular Risk in Young Finns Study.
Oikonen M, Wendelin-Saarenhovi M, Siitonen N, Sainio A, Juonala M, Kahonen M, Lyytikainen LP, Seppala I, Lehtimaki T, Viikari JS, Jarvelainen H, Raitakari OT., Scand. J. Clin. Lab. Invest. 72(7), 2012
PMID: 22978719
Matrix metalloproteinase protein expression profiles cannot distinguish between normal and early osteoarthritic synovial fluid.
Heard BJ, Martin L, Rattner JB, Frank CB, Hart DA, Krawetz R., BMC Musculoskelet Disord 13(), 2012
PMID: 22824140
Bioengineering embryonic stem cell microenvironments for the study of breast cancer.
Raof NA, Mooney BM, Xie Y., Int J Mol Sci 12(11), 2011
PMID: 22174624
Mice with tissue inhibitor of metalloproteinases 4 (Timp4) deletion succumb to induced myocardial infarction but not to cardiac pressure overload.
Koskivirta I, Kassiri Z, Rahkonen O, Kiviranta R, Oudit GY, McKee TD, Kyto V, Saraste A, Jokinen E, Liu PP, Vuorio E, Khokha R., J. Biol. Chem. 285(32), 2010
PMID: 20516072
Modulation of angiogenesis by tissue inhibitor of metalloproteinase-4.
Fernandez CA, Moses MA., Biochem. Biophys. Res. Commun. 345(1), 2006
PMID: 16682001
The TIMPs tango with MMPs and more in the central nervous system.
Crocker SJ, Pagenstecher A, Campbell IL., J. Neurosci. Res. 75(1), 2004
PMID: 14689443

26 References

Data provided by Europe PubMed Central.

Export

0 Marked Publications

Open Data PUB

Web of Science

View record in Web of Science®

Sources

PMID: 11501766
PubMed | Europe PMC

Search this title in

Google Scholar