Screening for trbB- and traG-like sequences by PCR for the detection of conjugative plasmids in bacterial soil isolates

Disque-Kochem C, Battermann A, Stratz M, Dreiseikelmann B (2001)
MICROBIOLOGICAL RESEARCH 156(2): 159-168.

Download
Es wurde kein Volltext hochgeladen. Nur Publikationsnachweis!
Zeitschriftenaufsatz | Veröffentlicht | Englisch
Autor
; ; ;
Abstract / Bemerkung
The transfer regions of different conjugative plasmids show significant similarities in the genetic organization and in the amino acid sequence of some gene products, especially of proteins from the traG or trbB family. These similarities are also evident on the level of the nucleotide sequences. On the basis of conserved DNA regions we designed degenerate PCR primer pairs to detect specifically tra regions within a collection of bacterial clones isolated from an agricultural soil. Most of the potential transfer-proficient indigenous bacterial isolates were able to mobilize a derivative of the nonconjugative IncQ plasmid RSF1010 into recipient strains. With the help of the primers it should be possible to evaluate the genetic potential for horizontal gene transfer carried out by conjugative plasmids.
Erscheinungsjahr
Zeitschriftentitel
MICROBIOLOGICAL RESEARCH
Band
156
Zeitschriftennummer
2
Seite
159-168
ISSN
PUB-ID

Zitieren

Disque-Kochem C, Battermann A, Stratz M, Dreiseikelmann B. Screening for trbB- and traG-like sequences by PCR for the detection of conjugative plasmids in bacterial soil isolates. MICROBIOLOGICAL RESEARCH. 2001;156(2):159-168.
Disque-Kochem, C., Battermann, A., Stratz, M., & Dreiseikelmann, B. (2001). Screening for trbB- and traG-like sequences by PCR for the detection of conjugative plasmids in bacterial soil isolates. MICROBIOLOGICAL RESEARCH, 156(2), 159-168. doi:10.1078/0944-5013-00098
Disque-Kochem, C., Battermann, A., Stratz, M., and Dreiseikelmann, B. (2001). Screening for trbB- and traG-like sequences by PCR for the detection of conjugative plasmids in bacterial soil isolates. MICROBIOLOGICAL RESEARCH 156, 159-168.
Disque-Kochem, C., et al., 2001. Screening for trbB- and traG-like sequences by PCR for the detection of conjugative plasmids in bacterial soil isolates. MICROBIOLOGICAL RESEARCH, 156(2), p 159-168.
C. Disque-Kochem, et al., “Screening for trbB- and traG-like sequences by PCR for the detection of conjugative plasmids in bacterial soil isolates”, MICROBIOLOGICAL RESEARCH, vol. 156, 2001, pp. 159-168.
Disque-Kochem, C., Battermann, A., Stratz, M., Dreiseikelmann, B.: Screening for trbB- and traG-like sequences by PCR for the detection of conjugative plasmids in bacterial soil isolates. MICROBIOLOGICAL RESEARCH. 156, 159-168 (2001).
Disque-Kochem, C, Battermann, A, Stratz, M, and Dreiseikelmann, Brigitte. “Screening for trbB- and traG-like sequences by PCR for the detection of conjugative plasmids in bacterial soil isolates”. MICROBIOLOGICAL RESEARCH 156.2 (2001): 159-168.

3 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Plasmid incidence in the subgroups of two bacterial communities.
Beilstein F, Battermann A, Dreiseikelmann B., Microbiol Res 163(3), 2008
PMID: 16872814
Transfer of antibiotic multiresistant plasmid RP4 from escherichia coli to activated sludge bacteria.
Soda S, Otsuki H, Inoue D, Tsutsui H, Sei K, Ike M., J Biosci Bioeng 106(3), 2008
PMID: 18930008

41 References

Daten bereitgestellt von Europe PubMed Central.

Distribution, diversity and evolution of the bacterial mercury resistance (mer) operon.
Osborn AM, Bruce KD, Strike P, Ritchie DA., FEMS Microbiol. Rev. 19(4), 1997
PMID: 9167257
Complete nucleotide sequence of Birmingham IncP alpha plasmids. Compilation and comparative analysis.
Pansegrau W, Lanka E, Barth PT, Figurski DH, Guiney DG, Haas D, Helinski DR, Schwab H, Stanisich VA, Thomas CM., J. Mol. Biol. 239(5), 1994
PMID: 8014987
Conjugational transfer of recombinant DNA in cultures and in soils: host range of Pseudomonas putida TOL plasmids.
Ramos-Gonzalez MI, Duque E, Ramos JL., Appl. Environ. Microbiol. 57(10), 1991
PMID: 1660698

Sambrook, 1989
Transferable drug resistance in bacteria from fish-farm sediments
Sandaa, Can. J. Microbiol. 38(), 1992
A broad host range mobilization system for in vivo genetic engineering: transposon mutagenesis in gram negative bacteria
Simon, Biotechnology 1(), 1983
Detection of RPa transfer in soil and rhizoshere, and the occurrence of homology to RP4 in soil bacteria
van, Curr. Microbiol. 19(), 1989
Antibiotic resistance gene transfer in the environment: An overview
van, 1992
Mobilization of the nonconjugative IncQ plasmid RSF1010
Willetts, Genet. Res. Camb. 37(), 1981
Conjugal transfer system of the IncN plasmid pKM101.
Winans SC, Walker GC., J. Bacteriol. 161(1), 1985
PMID: 2857162

Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®

Quellen

PMID: 11572456
PubMed | Europe PMC

Suchen in

Google Scholar