A highly efficient transposon mutagenesis system for the tomato pathogen Clavibacter michiganensis subsp michiganensis

Kirchner O, Gartemann K-H, Zellermann EM, Eichenlaub R, Burger A (2001)
MOLECULAR PLANT-MICROBE INTERACTIONS 14(11): 1312-1318.

Download
No fulltext has been uploaded. References only!
Journal Article | Original Article | Published | English

No fulltext has been uploaded

Author
; ; ; ;
Abstract / Notes
A transposon mutagenesis system for Clavibacter michiganensis subsp. michiganensis was developed based on antibiotic resistance transposons that were derived from the insertion element IS1409 from Arthrobacter sp. strain TM1 NCIB12013. As a prerequisite, the electroporation efficiency was optimized by using unmethylated DNA and treatment of the cells with glycine such that about 5 x 10(6) transformants per jag of DNA were generally obtained. Electroporation of C. michiganensis subsp. michiganensis with a suicide vector carrying transposon Tn1409C resulted in approximately 1 x 10(3) transposon mutants per mug of DNA and thus is suitable for saturation mutagenesis. Analysis of Tn1409C insertion sites suggests a random mode of transposition. Transposition of Tn1409C was also demonstrated for other subspecies of C. michiganensis.
Publishing Year
ISSN
PUB-ID

Cite this

Kirchner O, Gartemann K-H, Zellermann EM, Eichenlaub R, Burger A. A highly efficient transposon mutagenesis system for the tomato pathogen Clavibacter michiganensis subsp michiganensis. MOLECULAR PLANT-MICROBE INTERACTIONS. 2001;14(11):1312-1318.
Kirchner, O., Gartemann, K. - H., Zellermann, E. M., Eichenlaub, R., & Burger, A. (2001). A highly efficient transposon mutagenesis system for the tomato pathogen Clavibacter michiganensis subsp michiganensis. MOLECULAR PLANT-MICROBE INTERACTIONS, 14(11), 1312-1318. doi:10.1094/MPMI.2001.14.11.1312
Kirchner, O., Gartemann, K. - H., Zellermann, E. M., Eichenlaub, R., and Burger, A. (2001). A highly efficient transposon mutagenesis system for the tomato pathogen Clavibacter michiganensis subsp michiganensis. MOLECULAR PLANT-MICROBE INTERACTIONS 14, 1312-1318.
Kirchner, O., et al., 2001. A highly efficient transposon mutagenesis system for the tomato pathogen Clavibacter michiganensis subsp michiganensis. MOLECULAR PLANT-MICROBE INTERACTIONS, 14(11), p 1312-1318.
O. Kirchner, et al., “A highly efficient transposon mutagenesis system for the tomato pathogen Clavibacter michiganensis subsp michiganensis”, MOLECULAR PLANT-MICROBE INTERACTIONS, vol. 14, 2001, pp. 1312-1318.
Kirchner, O., Gartemann, K.-H., Zellermann, E.M., Eichenlaub, R., Burger, A.: A highly efficient transposon mutagenesis system for the tomato pathogen Clavibacter michiganensis subsp michiganensis. MOLECULAR PLANT-MICROBE INTERACTIONS. 14, 1312-1318 (2001).
Kirchner, O, Gartemann, Karl-Heinz, Zellermann, EM, Eichenlaub, Rudolf, and Burger, A. “A highly efficient transposon mutagenesis system for the tomato pathogen Clavibacter michiganensis subsp michiganensis”. MOLECULAR PLANT-MICROBE INTERACTIONS 14.11 (2001): 1312-1318.
This data publication is cited in the following publications:
This publication cites the following data publications:

17 Citations in Europe PMC

Data provided by Europe PubMed Central.

Plant-like bacterial expansins play contrasting roles in two tomato vascular pathogens.
Tancos MA, Lowe-Power TM, Peritore-Galve FC, Tran TM, Allen C, Smart CD., Mol Plant Pathol 19(5), 2018
PMID: 28868644
Differential contribution of Clavibacter michiganensis ssp. michiganensis virulence factors to systemic and local infection in tomato.
Chalupowicz L, Barash I, Reuven M, Dror O, Sharabani G, Gartemann KH, Eichenlaub R, Sessa G, Manulis-Sasson S., Mol Plant Pathol 18(3), 2017
PMID: 26992141
Genomic Analysis of Clavibacter michiganensis Reveals Insight Into Virulence Strategies and Genetic Diversity of a Gram-Positive Bacterial Pathogen.
Thapa SP, Pattathil S, Hahn MG, Jacques MA, Gilbertson RL, Coaker G., Mol Plant Microbe Interact 30(10), 2017
PMID: 28677494
Clavibacter michiganensis subsp. michiganensis Vatr1 and Vatr2 Transcriptional Regulators Are Required for Virulence in Tomato.
Savidor A, Chalupowicz L, Teper D, Gartemann KH, Eichenlaub R, Manulis-Sasson S, Barash I, Sessa G., Mol Plant Microbe Interact 2015(1), 2015
PMID: 27839071
Studies on the colonization of axenically grown tomato plants by a GFP-tagged strain of Clavibacter michiganensis subsp. michiganensis
Lelis FMV, Czajkowski R, de Souza RM, Ribeiro DH, van der Wolf JM., Eur J Plant Pathol 139(1), 2014
PMID: IND500743147
Tomato fruit and seed colonization by Clavibacter michiganensis subsp. michiganensis through external and internal routes.
Tancos MA, Chalupowicz L, Barash I, Manulis-Sasson S, Smart CD., Appl Environ Microbiol 79(22), 2013
PMID: 24014525
Bioluminescence imaging of Clavibacter michiganensis subsp. michiganensis infection of tomato seeds and plants.
Xu X, Miller SA, Baysal-Gurel F, Gartemann KH, Eichenlaub R, Rajashekara G., Appl Environ Microbiol 76(12), 2010
PMID: 20400561
The putative secreted serine protease Chp-7 is required for full virulence and induction of a nonhost hypersensitive response by Clavibacter michiganensis subsp. sepedonicus.
Nissinen R, Xia Y, Mattinen L, Ishimaru CA, Knudson DL, Knudson SE, Metzler M, Pirhonen M., Mol Plant Microbe Interact 22(7), 2009
PMID: 19522563
The genome sequence of the tomato-pathogenic actinomycete Clavibacter michiganensis subsp. michiganensis NCPPB382 reveals a large island involved in pathogenicity.
Gartemann KH, Abt B, Bekel T, Burger A, Engemann J, Flügel M, Gaigalat L, Goesmann A, Gräfen I, Kalinowski J, Kaup O, Kirchner O, Krause L, Linke B, McHardy A, Meyer F, Pohle S, Rückert C, Schneiker S, Zellermann EM, Pühler A, Eichenlaub R, Kaiser O, Bartels D., J Bacteriol 190(6), 2008
PMID: 18192381
Genome of the actinomycete plant pathogen Clavibacter michiganensis subsp. sepedonicus suggests recent niche adaptation.
Bentley SD, Corton C, Brown SE, Barron A, Clark L, Doggett J, Harris B, Ormond D, Quail MA, May G, Francis D, Knudson D, Parkhill J, Ishimaru CA., J Bacteriol 190(6), 2008
PMID: 18192393
Identification of homologues to the pathogenicity factor Pat-1, a putative serine protease of Clavibacter michiganensis subsp. michiganensis.
Burger A, Gräfen I, Engemann J, Niermann E, Pieper M, Kirchner O, Gartemann KH, Eichenlaub R., Microbiol Res 160(4), 2005
PMID: 16255147
Identification of a tomatinase in the tomato-pathogenic actinomycete Clavibacter michiganensis subsp. michiganensis NCPPB382.
Kaup O, Gräfen I, Zellermann EM, Eichenlaub R, Gartemann KH., Mol Plant Microbe Interact 18(10), 2005
PMID: 16255248
Clavibacter michiganensis subsp. michiganensis: first steps in the understanding of virulence of a Gram-positive phytopathogenic bacterium.
Gartemann KH, Kirchner O, Engemann J, Gräfen I, Eichenlaub R, Burger A., J Biotechnol 106(2-3), 2003
PMID: 14651860

15 References

Data provided by Europe PubMed Central.

Gapped BLAST and PSI-BLAST: a new generation of protein database search programs.
Altschul SF, Madden TL, Schaffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ., Nucleic Acids Res. 25(17), 1997
PMID: 9254694
Proposal for a New Hierarchic Classification System, Actinobacteria classis nov.
STACKEBRANDT, International Journal of Systematic Bacteriology 47(2), 1997
Replacement Recombination in Coryneform Bacteria: High Efficiency Integration Requirement for Non-methylated Plasmid DNA.
Vertes, Bioscience Biotechnology and Biochemistry 57(12), 1993
Presence of mrr- and mcr-like restriction systems in coryneform bacteria.
Vertes AA, Inui M, Kobayashi M, Kurusu Y, Yukawa H., Res. Microbiol. 144(3), 1993
PMID: 8210675
Corynebacterium glutamicum DNA is subjected to methylation-restriction in Escherichia coli.
Tauch A, Kirchner O, Wehmeier L, Kalinowski J, Puhler A., FEMS Microbiol. Lett. 123(3), 1994
PMID: 7988915
Electrotransformation of Brevibacterium lactofermentum and Corynebacterium glutamicum: growth in tween 80 increases transformation frequencies
Haynes, FEMS Microbiology Letters 61(3), 1989

Export

0 Marked Publications

Open Data PUB

Web of Science

View record in Web of Science®

Sources

PMID: 11763129
PubMed | Europe PMC

Search this title in

Google Scholar