A highly efficient transposon mutagenesis system for the tomato pathogen Clavibacter michiganensis subsp michiganensis

Kirchner O, Gartemann K-H, Zellermann EM, Eichenlaub R, Burger A (2001)
MOLECULAR PLANT-MICROBE INTERACTIONS 14(11): 1312-1318.

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Abstract
A transposon mutagenesis system for Clavibacter michiganensis subsp. michiganensis was developed based on antibiotic resistance transposons that were derived from the insertion element IS1409 from Arthrobacter sp. strain TM1 NCIB12013. As a prerequisite, the electroporation efficiency was optimized by using unmethylated DNA and treatment of the cells with glycine such that about 5 x 10(6) transformants per jag of DNA were generally obtained. Electroporation of C. michiganensis subsp. michiganensis with a suicide vector carrying transposon Tn1409C resulted in approximately 1 x 10(3) transposon mutants per mug of DNA and thus is suitable for saturation mutagenesis. Analysis of Tn1409C insertion sites suggests a random mode of transposition. Transposition of Tn1409C was also demonstrated for other subspecies of C. michiganensis.
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Kirchner O, Gartemann K-H, Zellermann EM, Eichenlaub R, Burger A. A highly efficient transposon mutagenesis system for the tomato pathogen Clavibacter michiganensis subsp michiganensis. MOLECULAR PLANT-MICROBE INTERACTIONS. 2001;14(11):1312-1318.
Kirchner, O., Gartemann, K. - H., Zellermann, E. M., Eichenlaub, R., & Burger, A. (2001). A highly efficient transposon mutagenesis system for the tomato pathogen Clavibacter michiganensis subsp michiganensis. MOLECULAR PLANT-MICROBE INTERACTIONS, 14(11), 1312-1318.
Kirchner, O., Gartemann, K. - H., Zellermann, E. M., Eichenlaub, R., and Burger, A. (2001). A highly efficient transposon mutagenesis system for the tomato pathogen Clavibacter michiganensis subsp michiganensis. MOLECULAR PLANT-MICROBE INTERACTIONS 14, 1312-1318.
Kirchner, O., et al., 2001. A highly efficient transposon mutagenesis system for the tomato pathogen Clavibacter michiganensis subsp michiganensis. MOLECULAR PLANT-MICROBE INTERACTIONS, 14(11), p 1312-1318.
O. Kirchner, et al., “A highly efficient transposon mutagenesis system for the tomato pathogen Clavibacter michiganensis subsp michiganensis”, MOLECULAR PLANT-MICROBE INTERACTIONS, vol. 14, 2001, pp. 1312-1318.
Kirchner, O., Gartemann, K.-H., Zellermann, E.M., Eichenlaub, R., Burger, A.: A highly efficient transposon mutagenesis system for the tomato pathogen Clavibacter michiganensis subsp michiganensis. MOLECULAR PLANT-MICROBE INTERACTIONS. 14, 1312-1318 (2001).
Kirchner, O, Gartemann, Karl-Heinz, Zellermann, EM, Eichenlaub, Rudolf, and Burger, A. “A highly efficient transposon mutagenesis system for the tomato pathogen Clavibacter michiganensis subsp michiganensis”. MOLECULAR PLANT-MICROBE INTERACTIONS 14.11 (2001): 1312-1318.
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7 Citations in Europe PMC

Data provided by Europe PubMed Central.

Tomato fruit and seed colonization by Clavibacter michiganensis subsp. michiganensis through external and internal routes.
Tancos MA, Chalupowicz L, Barash I, Manulis-Sasson S, Smart CD., Appl. Environ. Microbiol. 79(22), 2013
PMID: 24014525
Bioluminescence imaging of Clavibacter michiganensis subsp. michiganensis infection of tomato seeds and plants.
Xu X, Miller SA, Baysal-Gurel F, Gartemann KH, Eichenlaub R, Rajashekara G., Appl. Environ. Microbiol. 76(12), 2010
PMID: 20400561
Genome of the actinomycete plant pathogen Clavibacter michiganensis subsp. sepedonicus suggests recent niche adaptation.
Bentley SD, Corton C, Brown SE, Barron A, Clark L, Doggett J, Harris B, Ormond D, Quail MA, May G, Francis D, Knudson D, Parkhill J, Ishimaru CA., J. Bacteriol. 190(6), 2008
PMID: 18192393
The genome sequence of the tomato-pathogenic actinomycete Clavibacter michiganensis subsp. michiganensis NCPPB382 reveals a large island involved in pathogenicity.
Gartemann KH, Abt B, Bekel T, Burger A, Engemann J, Flugel M, Gaigalat L, Goesmann A, Grafen I, Kalinowski J, Kaup O, Kirchner O, Krause L, Linke B, McHardy A, Meyer F, Pohle S, Ruckert C, Schneiker S, Zellermann EM, Puhler A, Eichenlaub R, Kaiser O, Bartels D., J. Bacteriol. 190(6), 2008
PMID: 18192381
Identification of a tomatinase in the tomato-pathogenic actinomycete Clavibacter michiganensis subsp. michiganensis NCPPB382.
Kaup O, Grafen I, Zellermann EM, Eichenlaub R, Gartemann KH., Mol. Plant Microbe Interact. 18(10), 2005
PMID: 16255248
Clavibacter michiganensis subsp. michiganensis: first steps in the understanding of virulence of a Gram-positive phytopathogenic bacterium.
Gartemann KH, Kirchner O, Engemann J, Grafen I, Eichenlaub R, Burger A., J. Biotechnol. 106(2-3), 2003
PMID: 14651860

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