Strategy to sequence the genome of Corynebacterium glutamicum ATCC 13032: use of a cosmid and a bacterial artificial chromosome library

Tauch A, Homann I, Mormann S, Rüberg S, Billault A, Bathe B, Brand S, Brockmann-Gretza O, Rückert C, Schischka N, Wrenger C, et al. (2002)
JOURNAL OF BIOTECHNOLOGY 95(1): 25-38.

Download
No fulltext has been uploaded. References only!
Journal Article | Original Article | Published | English

No fulltext has been uploaded

Author
; ; ; ; ; ; ; ; ; ; ;
All
Abstract / Notes
The initial strategy of the Corynebacterium glutamicum genome project was to sequence overlapping inserts of an ordered cosmid library. High-density colony grids of approximately 28 genome equivalents were used for the identification of overlapping clones by Southern hybridization. Altogether 18 contiguous genomic segments comprising 95 overlapping cosmids were assembled. Systematic shotgun sequencing of the assembled cosmid set revealed that only 2.84 Mb (86.6%) of the C glutamicum genome were represented by the cosmid library. To obtain a complete genome coverage, a bacterial artificial chromosome (BAC) library of the C. glutamicum chromosome was constructed in pBeloBAC11 and used for genome mapping. The BAC library consists of 3168 BACs and represents a theoretical 63-fold coverage of the C. glutamicum genome (3.28 Mb). Southern screening of 2304 BAC clones with PCR-amplified chromosomal markers and subsequent insert terminal sequencing allowed the identification of 119 BACs covering the entire chromosome of C. glutamicum. The minimal set representing a 100% genome coverage contains 44 unique BAC clones with an average overlap of 22 kb. A total of 21 BACs represented linking clones between previously sequenced cosmid contigs and provided a valuable tool for completing the genome sequence of C. glutamicum. (C) 2002 Elsevier Science B.V. All rights reserved.
Publishing Year
ISSN
PUB-ID

Cite this

Tauch A, Homann I, Mormann S, et al. Strategy to sequence the genome of Corynebacterium glutamicum ATCC 13032: use of a cosmid and a bacterial artificial chromosome library. JOURNAL OF BIOTECHNOLOGY. 2002;95(1):25-38.
Tauch, A., Homann, I., Mormann, S., Rüberg, S., Billault, A., Bathe, B., Brand, S., et al. (2002). Strategy to sequence the genome of Corynebacterium glutamicum ATCC 13032: use of a cosmid and a bacterial artificial chromosome library. JOURNAL OF BIOTECHNOLOGY, 95(1), 25-38. doi:10.1016/S0168-1656(01)00443-6
Tauch, A., Homann, I., Mormann, S., Rüberg, S., Billault, A., Bathe, B., Brand, S., Brockmann-Gretza, O., Rückert, C., Schischka, N., et al. (2002). Strategy to sequence the genome of Corynebacterium glutamicum ATCC 13032: use of a cosmid and a bacterial artificial chromosome library. JOURNAL OF BIOTECHNOLOGY 95, 25-38.
Tauch, A., et al., 2002. Strategy to sequence the genome of Corynebacterium glutamicum ATCC 13032: use of a cosmid and a bacterial artificial chromosome library. JOURNAL OF BIOTECHNOLOGY, 95(1), p 25-38.
A. Tauch, et al., “Strategy to sequence the genome of Corynebacterium glutamicum ATCC 13032: use of a cosmid and a bacterial artificial chromosome library”, JOURNAL OF BIOTECHNOLOGY, vol. 95, 2002, pp. 25-38.
Tauch, A., Homann, I., Mormann, S., Rüberg, S., Billault, A., Bathe, B., Brand, S., Brockmann-Gretza, O., Rückert, C., Schischka, N., Wrenger, C., Hoheisel, J., Mockel, B., Huthmacher, K., Pfefferle, W., Pühler, A., Kalinowski, M.: Strategy to sequence the genome of Corynebacterium glutamicum ATCC 13032: use of a cosmid and a bacterial artificial chromosome library. JOURNAL OF BIOTECHNOLOGY. 95, 25-38 (2002).
Tauch, Andreas, Homann, I, Mormann, S, Rüberg, Silvia, Billault, A, Bathe, B, Brand, S, Brockmann-Gretza, O, Rückert, Christian, Schischka, N, Wrenger, C, Hoheisel, J, Mockel, B, Huthmacher, K, Pfefferle, W, Pühler, Alfred, and Kalinowski, M. “Strategy to sequence the genome of Corynebacterium glutamicum ATCC 13032: use of a cosmid and a bacterial artificial chromosome library”. JOURNAL OF BIOTECHNOLOGY 95.1 (2002): 25-38.
This data publication is cited in the following publications:
This publication cites the following data publications:

37 Citations in Europe PMC

Data provided by Europe PubMed Central.

The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of L-aspartate-derived amino acids and vitamins.
Kalinowski J, Bathe B, Bartels D, Bischoff N, Bott M, Burkovski A, Dusch N, Eggeling L, Eikmanns BJ, Gaigalat L, Goesmann A, Hartmann M, Huthmacher K, Krämer R, Linke B, McHardy AC, Meyer F, Möckel B, Pfefferle W, Pühler A, Rey DA, Rückert C, Rupp O, Sahm H, Wendisch VF, Wiegräbe I, Tauch A., J Biotechnol 104(1-3), 2003
PMID: 12948626
Ribosomal RNA and ribosomal proteins in corynebacteria.
Martín JF, Barreiro C, González-Lavado E, Barriuso M., J Biotechnol 104(1-3), 2003
PMID: 12948628
Acetate metabolism and its regulation in Corynebacterium glutamicum.
Gerstmeir R, Wendisch VF, Schnicke S, Ruan H, Farwick M, Reinscheid D, Eikmanns BJ., J Biotechnol 104(1-3), 2003
PMID: 12948633
Genome-based analysis of biosynthetic aminotransferase genes of Corynebacterium glutamicum.
McHardy AC, Tauch A, Rückert C, Pühler A, Kalinowski J., J Biotechnol 104(1-3), 2003
PMID: 12948641
Whole genome shotgun sequencing guided by bioinformatics pipelines--an optimized approach for an established technique.
Kaiser O, Bartels D, Bekel T, Goesmann A, Kespohl S, Pühler A, Meyer F., J Biotechnol 106(2-3), 2003
PMID: 14651855
Building a BRIDGE for the integration of heterogeneous data from functional genomics into a platform for systems biology.
Goesmann A, Linke B, Rupp O, Krause L, Bartels D, Dondrup M, McHardy AC, Wilke A, Pühler A, Meyer F., J Biotechnol 106(2-3), 2003
PMID: 14651858

27 References

Data provided by Europe PubMed Central.

The role of the Corynebacterium glutamicum rel gene in (p)ppGpp metabolism.
Wehmeier L, Schafer A, Burkovski A, Kramer R, Mechold U, Malke H, Puhler A, Kalinowski J., Microbiology (Reading, Engl.) 144 ( Pt 7)(), 1998
PMID: 9695918
Construction and characterization of a bacterial artificial chromosome library of Sorghum bicolor.
Woo SS, Jiang J, Gill BS, Paterson AH, Wing RA., Nucleic Acids Res. 22(23), 1994
PMID: 7800481

Export

0 Marked Publications

Open Data PUB

Web of Science

View record in Web of Science®

Sources

PMID: 11879709
PubMed | Europe PMC

Search this title in

Google Scholar