Identification of the active site of gelatinase B as the structural element sufficient for converting a protein to a metalloprotease

Kaur K, Zhu KJ, Whittemore MS, Petersen RL, Lichte A, Tschesche H, Pourmotabbed T (2002)
BIOCHEMISTRY 41(15): 4789-4797.

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Abstract
Gelatinase B is a member of the matrix metalloproteinase family that efficiently cleaves gelatin, elastin, and types V and X collagen. To understand the contribution of the active site of the enzyme (amino acid residues 373-456) in these activities, we studied catalytic properties of a fusion protein consisting of maltose binding protein and the active site region of gelatinase B. We found that addition of the active site of gelatinase B, which corresponds to 12% of the total protein molecule, to maltose binding protein is sufficient to endow the protein with the ability to cleave the peptide substrates Mca-PLGL(Dpa)AR-NH2 and DNP-PLGLWA-(D)-R-NH2. The fusion protein hydrolyzed the Mca-PLGL(Dpa)AR-NH2 peptide with the same efficiency as that of the stromelysin, k(cat)/K-m similar or equal to 1.07 x 10(6) M-1 h(-1). The fusion protein, however, was not able to degrade the large substrate, gelatin. Inhibition of the activity of the protein by EDTA suggested that its activity was metal dependent. ESR analyses indicated that the fusion protein bound one molecule of Zn2+. In addition, Z-Pro-Leu-Gly-hydroxamate and TIMP-1 inhibited the activity of the protein, suggesting that the structure of the active site of the fusion protein is similar to that of the other metalloproteinases. These data provide fundamental information about the structural elements required for transforming a protein to a metalloprotease.
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Kaur K, Zhu KJ, Whittemore MS, et al. Identification of the active site of gelatinase B as the structural element sufficient for converting a protein to a metalloprotease. BIOCHEMISTRY. 2002;41(15):4789-4797.
Kaur, K., Zhu, K. J., Whittemore, M. S., Petersen, R. L., Lichte, A., Tschesche, H., & Pourmotabbed, T. (2002). Identification of the active site of gelatinase B as the structural element sufficient for converting a protein to a metalloprotease. BIOCHEMISTRY, 41(15), 4789-4797.
Kaur, K., Zhu, K. J., Whittemore, M. S., Petersen, R. L., Lichte, A., Tschesche, H., and Pourmotabbed, T. (2002). Identification of the active site of gelatinase B as the structural element sufficient for converting a protein to a metalloprotease. BIOCHEMISTRY 41, 4789-4797.
Kaur, K., et al., 2002. Identification of the active site of gelatinase B as the structural element sufficient for converting a protein to a metalloprotease. BIOCHEMISTRY, 41(15), p 4789-4797.
K. Kaur, et al., “Identification of the active site of gelatinase B as the structural element sufficient for converting a protein to a metalloprotease”, BIOCHEMISTRY, vol. 41, 2002, pp. 4789-4797.
Kaur, K., Zhu, K.J., Whittemore, M.S., Petersen, R.L., Lichte, A., Tschesche, H., Pourmotabbed, T.: Identification of the active site of gelatinase B as the structural element sufficient for converting a protein to a metalloprotease. BIOCHEMISTRY. 41, 4789-4797 (2002).
Kaur, K, Zhu, KJ, Whittemore, MS, Petersen, RL, Lichte, A, Tschesche, Harald, and Pourmotabbed, T. “Identification of the active site of gelatinase B as the structural element sufficient for converting a protein to a metalloprotease”. BIOCHEMISTRY 41.15 (2002): 4789-4797.
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PMID: 17206645

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