Modulation of the catalytic activity of neutrophil collagenase MMP-8 on bovine collagen I - Role of the activation cleavage and of the hemopexin-like domain

Gioia M, Fasciglione GF, Marini S, D'Alessio S, De Sanctis G, Diekmann O, Pieper M, Politi V, Tschesche H, Coletta M (2002)
JOURNAL OF BIOLOGICAL CHEMISTRY 277(26): 23123-23130.

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Abstract
The cleavage of bovine collagen I by neutrophil collagenase MMP-8 has been followed at pH 7.4, 37 degreesC. The behavior of the whole enzyme molecule (whMMP-8), displaying both the catalytic domain and the hemopexin-like domain, has been compared under the same experimental conditions with that of the catalytic domain only. The main observation is that whMMP-8 cleaves bovine collagen I only at a single specific site, as already reported by many others (Mallya, S. K., Mookhtiar, K. A., Gao, Y., Brew, K., Dioszegi, M., Birkedal-Hansen, H., and van Wart, H. E. (1990) Biochemistry 29, 10628-10634; Knauper, V., Osthues, A., DeClerk, Y. A., Langley, K. A, Blaser, J., and Tschesche, H. (1993) Biochem. J. 291, 847854; Marini, S., Fasciglione, G. F., De Sanctis, G., D'Alessio, S., Politi, V., and Coletta, M. (2000) J. Biol. Chem. 275, 18657-18663), whereas the catalytic domain lacks this specificity and cleaves the collagen molecule at multiple sites. Furthermore, a meaningful difference is observed for the cleavage features displayed by two forms of the catalytic domain, which differ for the N terminus resulting from the activation process (i.e. the former Met(80)of the proenzyme (MetMMP-8) and the former Phe(79) of the proenzyme (PheMMP-8)). Thus, the PheMMP-8 species is characterized by a much faster k(cat)/K-m, fully attributable to a lower K-m, suggesting that the conformation of the catalytic domain, induced by the insertion of this N-terminal residue in a specific pocket (Reinemer, P., Grams, F., Huber, R., Kleine, T., Schnierer, S., Piper, M., Tschesche, H., and Bode, W. (1994) FEBS Lett. 338, 227-233), brings about a better, although less discriminatory, recognition process of cleavage site(s) on bovine collagen I.
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Gioia M, Fasciglione GF, Marini S, et al. Modulation of the catalytic activity of neutrophil collagenase MMP-8 on bovine collagen I - Role of the activation cleavage and of the hemopexin-like domain. JOURNAL OF BIOLOGICAL CHEMISTRY. 2002;277(26):23123-23130.
Gioia, M., Fasciglione, G. F., Marini, S., D'Alessio, S., De Sanctis, G., Diekmann, O., Pieper, M., et al. (2002). Modulation of the catalytic activity of neutrophil collagenase MMP-8 on bovine collagen I - Role of the activation cleavage and of the hemopexin-like domain. JOURNAL OF BIOLOGICAL CHEMISTRY, 277(26), 23123-23130.
Gioia, M., Fasciglione, G. F., Marini, S., D'Alessio, S., De Sanctis, G., Diekmann, O., Pieper, M., Politi, V., Tschesche, H., and Coletta, M. (2002). Modulation of the catalytic activity of neutrophil collagenase MMP-8 on bovine collagen I - Role of the activation cleavage and of the hemopexin-like domain. JOURNAL OF BIOLOGICAL CHEMISTRY 277, 23123-23130.
Gioia, M., et al., 2002. Modulation of the catalytic activity of neutrophil collagenase MMP-8 on bovine collagen I - Role of the activation cleavage and of the hemopexin-like domain. JOURNAL OF BIOLOGICAL CHEMISTRY, 277(26), p 23123-23130.
M. Gioia, et al., “Modulation of the catalytic activity of neutrophil collagenase MMP-8 on bovine collagen I - Role of the activation cleavage and of the hemopexin-like domain”, JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 277, 2002, pp. 23123-23130.
Gioia, M., Fasciglione, G.F., Marini, S., D'Alessio, S., De Sanctis, G., Diekmann, O., Pieper, M., Politi, V., Tschesche, H., Coletta, M.: Modulation of the catalytic activity of neutrophil collagenase MMP-8 on bovine collagen I - Role of the activation cleavage and of the hemopexin-like domain. JOURNAL OF BIOLOGICAL CHEMISTRY. 277, 23123-23130 (2002).
Gioia, M, Fasciglione, GF, Marini, S, D'Alessio, S, De Sanctis, G, Diekmann, O, Pieper, M, Politi, V, Tschesche, Harald, and Coletta, M. “Modulation of the catalytic activity of neutrophil collagenase MMP-8 on bovine collagen I - Role of the activation cleavage and of the hemopexin-like domain”. JOURNAL OF BIOLOGICAL CHEMISTRY 277.26 (2002): 23123-23130.
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