The 27.8-kb R-plasmid pTET3 from Corynebacterium glutamicum encodes the aminoglycoside adenyltransferase gene cassette aadA9 and the regulated tetracycline efflux system Tet 33 flanked by active copies of the widespread insertion sequence IS6100

Tauch A, Götker S, Pühler A, Kalinowski J, Thierbach G (2002)
Plasmid 48(2): 117-129.

Journal Article | Published | English

No fulltext has been uploaded

Abstract
We determined the complete nucleotide sequence of the 27.8-kb R-plasmid pTET3 from Corynebacterium glutamicum LP-6 which encodes streptomycin, spectinomycin, and tetracycline resistance. The antibiotic resistance determinant of pTET3 comprises an intIl-like gene. which was truncated by the insertion sequence IS6100, and the novel aminoglycoside adenyltransferase gene cassette aadA9. The deduced AADA9 protein showed 61%, identity and 71% similarity to AADA6 of integron In51 from Pseudomonas aeruginosa. In addition. pTET3 carries the novel repressor-regulated tetracycline resistance determinant Tet 33 which revealed amino acid sequence homology to group I tetracycline efflux systems. The highest level of similarity was observed to the tetracycline efflux protein TetA(Z) from the C glutamicum plasmid pAGI with 65% identical and 77%, similar amino acids. Each antibiotic resistance region of pTET3 is flanked by identical copies of the widespread insertion sequence IS6100 initially identified in Mycobacterium fortuitum. Transposition assays with a cloned copy of IS6100 revealed that this element is transpositionally active in C glutamicum. These data suggest a central role of IS6100 in the evolutionary history of pTET3 by mediating the cointegrative assembly of resistance gene-carrying DNA segments. (C) 2002 Elsevier Science (USA). All rights reserved.
Publishing Year
ISSN
PUB-ID

Cite this

Tauch A, Götker S, Pühler A, Kalinowski J, Thierbach G. The 27.8-kb R-plasmid pTET3 from Corynebacterium glutamicum encodes the aminoglycoside adenyltransferase gene cassette aadA9 and the regulated tetracycline efflux system Tet 33 flanked by active copies of the widespread insertion sequence IS6100. Plasmid. 2002;48(2):117-129.
Tauch, A., Götker, S., Pühler, A., Kalinowski, J., & Thierbach, G. (2002). The 27.8-kb R-plasmid pTET3 from Corynebacterium glutamicum encodes the aminoglycoside adenyltransferase gene cassette aadA9 and the regulated tetracycline efflux system Tet 33 flanked by active copies of the widespread insertion sequence IS6100. Plasmid, 48(2), 117-129.
Tauch, A., Götker, S., Pühler, A., Kalinowski, J., and Thierbach, G. (2002). The 27.8-kb R-plasmid pTET3 from Corynebacterium glutamicum encodes the aminoglycoside adenyltransferase gene cassette aadA9 and the regulated tetracycline efflux system Tet 33 flanked by active copies of the widespread insertion sequence IS6100. Plasmid 48, 117-129.
Tauch, A., et al., 2002. The 27.8-kb R-plasmid pTET3 from Corynebacterium glutamicum encodes the aminoglycoside adenyltransferase gene cassette aadA9 and the regulated tetracycline efflux system Tet 33 flanked by active copies of the widespread insertion sequence IS6100. Plasmid, 48(2), p 117-129.
A. Tauch, et al., “The 27.8-kb R-plasmid pTET3 from Corynebacterium glutamicum encodes the aminoglycoside adenyltransferase gene cassette aadA9 and the regulated tetracycline efflux system Tet 33 flanked by active copies of the widespread insertion sequence IS6100”, Plasmid, vol. 48, 2002, pp. 117-129.
Tauch, A., Götker, S., Pühler, A., Kalinowski, J., Thierbach, G.: The 27.8-kb R-plasmid pTET3 from Corynebacterium glutamicum encodes the aminoglycoside adenyltransferase gene cassette aadA9 and the regulated tetracycline efflux system Tet 33 flanked by active copies of the widespread insertion sequence IS6100. Plasmid. 48, 117-129 (2002).
Tauch, Andreas, Götker, Susanne, Pühler, Alfred, Kalinowski, Jörn, and Thierbach, Georg. “The 27.8-kb R-plasmid pTET3 from Corynebacterium glutamicum encodes the aminoglycoside adenyltransferase gene cassette aadA9 and the regulated tetracycline efflux system Tet 33 flanked by active copies of the widespread insertion sequence IS6100”. Plasmid 48.2 (2002): 117-129.
This data publication is cited in the following publications:
This publication cites the following data publications:

50 Citations in Europe PMC

Data provided by Europe PubMed Central.

Resistance integrons: class 1, 2 and 3 integrons.
Deng Y, Bao X, Ji L, Chen L, Liu J, Miao J, Chen D, Bian H, Li Y, Yu G., Ann. Clin. Microbiol. Antimicrob. 14(), 2015
PMID: 26487554
Characterization of new bacterial catabolic genes and mobile genetic elements by high throughput genetic screening of a soil metagenomic library.
Jacquiod S, Demaneche S, Franqueville L, Ausec L, Xu Z, Delmont TO, Dunon V, Cagnon C, Mandic-Mulec I, Vogel TM, Simonet P., J. Biotechnol. 190(), 2014
PMID: 24721211
High-throughput screening of a Corynebacterium glutamicum mutant library on genomic and metabolic level.
Reimer LC, Spura J, Schmidt-Hohagen K, Schomburg D., PLoS ONE 9(2), 2014
PMID: 24504095
Analysis of in vivo function of predicted isoenzymes:a metabolomic approach.
Choorapoikayil S, Schoepe J, Buchinger S, Schomburg D., OMICS 16(12), 2012
PMID: 23215805
Design and testing of a synthetic biology framework for genetic engineering of Corynebacterium glutamicum.
Ravasi P, Peiru S, Gramajo H, Menzella HG., Microb. Cell Fact. 11(), 2012
PMID: 23134565
Class 1 integrons in environments with different degrees of urbanization.
Nardelli M, Scalzo PM, Ramirez MS, Quiroga MP, Cassini MH, Centron D., PLoS ONE 7(6), 2012
PMID: 22761743
Diphtheria in Europe: current problems and new challenges.
Zakikhany K, Efstratiou A., Future Microbiol 7(5), 2012
PMID: 22568715
Antimicrobial drug resistance in Corynebacterium diphtheriae mitis.
Barraud O, Badell E, Denis F, Guiso N, Ploy MC., Emerging Infect. Dis. 17(11), 2011
PMID: 22099107
Class 1 integron in staphylococci.
Xu Z, Li L, Shi L, Shirtliff ME., Mol. Biol. Rep. 38(8), 2011
PMID: 21258866
Characterization of a 24-kb plasmid pCGR2 newly isolated from Corynebacterium glutamicum.
Tsuchida Y, Kimura S, Suzuki N, Inui M, Yukawa H., Appl. Microbiol. Biotechnol. 87(5), 2010
PMID: 20552356
The genes coding for the conversion of carbazole to catechol are flanked by IS6100 elements in Sphingomonas sp. strain XLDN2-5.
Gai Z, Wang X, Liu X, Tai C, Tang H, He X, Wu G, Deng Z, Xu P., PLoS ONE 5(4), 2010
PMID: 20368802
Characterization of a new 2.4-kb plasmid of Corynebacterium casei and development of stable corynebacterial cloning vector.
Tsuchida Y, Kimura S, Suzuki N, Inui M, Yukawa H., Appl. Microbiol. Biotechnol. 81(6), 2009
PMID: 18936936
Antimicrobial resistance of Campylobacter coli isolates from swine.
Ekkapobyotin C, Padungtod P, Chuanchuen R., Int. J. Food Microbiol. 128(2), 2008
PMID: 18945505
A novel bla(CTX-M-14) gene-harboring complex class 1 integron with an In4-like backbone structure from a clinical isolate of Escherichia coli.
Bae IK, Lee YH, Jeong HJ, Hong SG, Lee SH, Jeong SH., Diagn. Microbiol. Infect. Dis. 62(3), 2008
PMID: 18703304
Insertion sequence-based cassette PCR: cultivation-independent isolation of gamma-hexachlorocyclohexane-degrading genes from soil DNA.
Fuchu G, Ohtsubo Y, Ito M, Miyazaki R, Ono A, Nagata Y, Tsuda M., Appl. Microbiol. Biotechnol. 79(4), 2008
PMID: 18425509
Activity of Tigecycline against Coryneform Bacteria of Clinical Interest and Listeria monocytogenes.
Salas C, Calvo J, Martinez-Martinez L., Antimicrob. Agents Chemother. 52(4), 2008
PMID: 18227183
Tn6001, a transposon-like element containing the blaVIM-3-harboring integron In450.
Tseng SP, Hsueh PR, Tsai JC, Teng LJ., Antimicrob. Agents Chemother. 51(11), 2007
PMID: 17846142
Unnoticed spread of class 1 integrons in gram-positive clinical strains isolated in Guangzhou, China.
Shi L, Zheng M, Xiao Z, Asakura M, Su J, Li L, Yamasaki S., Microbiol. Immunol. 50(6), 2006
PMID: 16785718
Manipulating corynebacteria, from individual genes to chromosomes.
Vertes AA, Inui M, Yukawa H., Appl. Environ. Microbiol. 71(12), 2005
PMID: 16332735
Novel spectinomycin/streptomycin resistance gene, aadA14, from Pasteurella multocida.
Kehrenberg C, Catry B, Haesebrouck F, de Kruif A, Schwarz S., Antimicrob. Agents Chemother. 49(7), 2005
PMID: 15980396
Tn7 distribution in Helicobacter pylori: a selective paradox.
Crespo O, Catalano M, Pineiro S, Matteo M, Leanza A, Centron D., Int. J. Antimicrob. Agents 25(4), 2005
PMID: 15784316
Phylogeny of efflux-mediated tetracycline resistance genes and related proteins revisited.
Guillaume G, Ledent V, Moens W, Collard JM., Microb. Drug Resist. 10(1), 2004
PMID: 15140389
Tylosin resistance in Arcanobacterium pyogenes is encoded by an erm X determinant.
Jost BH, Field AC, Trinh HT, Songer JG, Billington SJ., Antimicrob. Agents Chemother. 47(11), 2003
PMID: 14576111

Export

0 Marked Publications

Open Data PUB

Web of Science

View record in Web of Science®

Sources

PMID: 12383729
PubMed | Europe PMC

Search this title in

Google Scholar