Localization of GFP in frozen sections from unfixed mouse tissues: Immobilization of a highly soluble marker protein by formaldehyde vapor

Jockusch H, Voigt S, Eberhard D (2003)
Journal of Histochemistry & Cytochemistry 51(3): 401-404.

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Green fluorescent protein (GFP) and its variants, such as enhanced GFP (EGFP), have been introduced into mammalian cells by transgenes, e.g., to distinguish donor from host cells after transplantation. Free GFP is extremely soluble and leaks out from liquid-covered cryostat sections so that fixation of whole organs before sectioning has been mandatory. This precludes the analysis of serial sections with respect to fixation-sensitive enzyme activities and antigens. We describe here a vapor fixation for sections from unfixed cryostat blocks of tissue that allows unrestricted enzyme and immunohistochemistry on adjacent sections, as demonstrated for cross-striated muscle and other tissues from EGFP transgenic "green mice" and for a transplantation experiment.
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Jockusch H, Voigt S, Eberhard D. Localization of GFP in frozen sections from unfixed mouse tissues: Immobilization of a highly soluble marker protein by formaldehyde vapor. Journal of Histochemistry & Cytochemistry. 2003;51(3):401-404.
Jockusch, H., Voigt, S., & Eberhard, D. (2003). Localization of GFP in frozen sections from unfixed mouse tissues: Immobilization of a highly soluble marker protein by formaldehyde vapor. Journal of Histochemistry & Cytochemistry, 51(3), 401-404.
Jockusch, H., Voigt, S., and Eberhard, D. (2003). Localization of GFP in frozen sections from unfixed mouse tissues: Immobilization of a highly soluble marker protein by formaldehyde vapor. Journal of Histochemistry & Cytochemistry 51, 401-404.
Jockusch, H., Voigt, S., & Eberhard, D., 2003. Localization of GFP in frozen sections from unfixed mouse tissues: Immobilization of a highly soluble marker protein by formaldehyde vapor. Journal of Histochemistry & Cytochemistry, 51(3), p 401-404.
H. Jockusch, S. Voigt, and D. Eberhard, “Localization of GFP in frozen sections from unfixed mouse tissues: Immobilization of a highly soluble marker protein by formaldehyde vapor”, Journal of Histochemistry & Cytochemistry, vol. 51, 2003, pp. 401-404.
Jockusch, H., Voigt, S., Eberhard, D.: Localization of GFP in frozen sections from unfixed mouse tissues: Immobilization of a highly soluble marker protein by formaldehyde vapor. Journal of Histochemistry & Cytochemistry. 51, 401-404 (2003).
Jockusch, Harald, Voigt, Sylvana, and Eberhard, Daniel. “Localization of GFP in frozen sections from unfixed mouse tissues: Immobilization of a highly soluble marker protein by formaldehyde vapor”. Journal of Histochemistry & Cytochemistry 51.3 (2003): 401-404.
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