Characterization of the 4-hydroxybenzoyl-coenzyme A thioesterase from Arthrobacter sp strain SU

Zhuang ZH, Gartemann K-H, Eichenlaub R, Dunaway-Mariano D (2003)
APPLIED AND ENVIRONMENTAL MICROBIOLOGY 69(5): 2707-2711.

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Abstract
The Arthrobacter sp. strain SU 4-chlorobenzoate (4-CBA) dehalogenation pathway converts 4-CBA to 4-hydroxybenzoate (4-HBA). The pathway operon contains the genes fcbA, fcbB, and fcbC (A. Schmitz, K. H. Gartemann, J. Fiedler, E. Grund, and R. Eichenlaub, Appl. Environ. Microbiol. 58:4068-4071,1992). Genes fcbA and fcbB encode 4-CBA-coenzyme A (CoA) ligase and 4-CBA-CoA dehalogenase, respectively, whereas the function of fcbC is not known. We subcloned fcbC and expressed it in Escherichia coli, and we purified and characterized the FcbC protein. A substrate activity screen identified benzoyl-CoA thioesters as the most active substrates. Catalysis of 4-HBA-CoA hydrolysis to 4-HBA and CoA occurred with a k(cat) of 6.7 s(-1) and a K-m of 1.2 muM. The k(cat) pH rate profile for 4-HBA-CoA hydrolysis indicated optimal activity over a pH range of 6 to 10. The amino acid sequence of the FcbC protein was compared to other sequences contained in the protein sequence data banks. A large number of sequence homologues of unknown function were identified. On the other hand, the 4-HBA-CoA thioesterases isolated from 4-CBA-degrading Pseudomonas strains did not share significant sequence identity with the FcbC protein, indicating early divergence of the thioesterase-encoding genes.
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Zhuang ZH, Gartemann K-H, Eichenlaub R, Dunaway-Mariano D. Characterization of the 4-hydroxybenzoyl-coenzyme A thioesterase from Arthrobacter sp strain SU. APPLIED AND ENVIRONMENTAL MICROBIOLOGY. 2003;69(5):2707-2711.
Zhuang, Z. H., Gartemann, K. - H., Eichenlaub, R., & Dunaway-Mariano, D. (2003). Characterization of the 4-hydroxybenzoyl-coenzyme A thioesterase from Arthrobacter sp strain SU. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 69(5), 2707-2711.
Zhuang, Z. H., Gartemann, K. - H., Eichenlaub, R., and Dunaway-Mariano, D. (2003). Characterization of the 4-hydroxybenzoyl-coenzyme A thioesterase from Arthrobacter sp strain SU. APPLIED AND ENVIRONMENTAL MICROBIOLOGY 69, 2707-2711.
Zhuang, Z.H., et al., 2003. Characterization of the 4-hydroxybenzoyl-coenzyme A thioesterase from Arthrobacter sp strain SU. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 69(5), p 2707-2711.
Z.H. Zhuang, et al., “Characterization of the 4-hydroxybenzoyl-coenzyme A thioesterase from Arthrobacter sp strain SU”, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 69, 2003, pp. 2707-2711.
Zhuang, Z.H., Gartemann, K.-H., Eichenlaub, R., Dunaway-Mariano, D.: Characterization of the 4-hydroxybenzoyl-coenzyme A thioesterase from Arthrobacter sp strain SU. APPLIED AND ENVIRONMENTAL MICROBIOLOGY. 69, 2707-2711 (2003).
Zhuang, ZH, Gartemann, Karl-Heinz, Eichenlaub, Rudolf, and Dunaway-Mariano, D. “Characterization of the 4-hydroxybenzoyl-coenzyme A thioesterase from Arthrobacter sp strain SU”. APPLIED AND ENVIRONMENTAL MICROBIOLOGY 69.5 (2003): 2707-2711.
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