Striking complexity of lipopolysaccharide defects in a collection of Sinorhizobium meliloti mutants

Campbell GRO, Sharypova LA, Scheidle H, Jones KM, Niehaus K, Becker A, Walker GC (2003)
Journal of Bacteriology 185(13): 3853-3862.

Journal Article | Published | English

No fulltext has been uploaded

Author
; ; ; ; ; ;
Abstract
Although the role that lipopolysaccharide (LPS) plays in the symbiosis between Sinorhizobium meliloti and alfalfa has been studied for over a decade, its function in this process remains controversial and poorly understood. This is largely due to a lack of mutants affected by its synthesis. In one of the definitive studies concerning this issue, Clover et al. (R. H. Clover, J. Kieber, and E. R. Signer, J. Bacteriol. 171:3961-3967, 1989) identified a series of mutants with putative LPS defects, judged them to be symbiotically proficient on Medicago sativa, and concluded that LPS might not have a symbiotic function in S. meliloti. The mutations in these strains were never characterized at the molecular level nor was the LPS from most of them analyzed. We have transduced these mutations from the Rm2011 background from which they were originally isolated into the sequenced strain Rm1021 and have characterized the resulting strains in greater detail. We found the LPS from these mutants to display a striking complexity of phenotypes on polyacrylamide electrophoresis gels, including additional rough LPS bands and alterations in the molecular weight distribution of the smooth LPS. We found that some of the mutants contain insertions in genes that are predicted to be involved in the synthesis of carbohydrate components of LPS, including ddhB, IpsB, lpsC, and lpsE. The majority, however, code for proteins predicted to be involved in a wide variety of functions not previously recognized to play a role in LPS synthesis, including a possible transcription elongation factor (GreA), a possible queuine synthesis protein, and a possible chemotaxis protein. Furthermore, using more extensive assays, we have found that most of these strains have symbiotic deficiencies. These results support more recent findings that alterations in LPS structure can affect the ability of S. meliloti to form an effective symbiosis.
Publishing Year
PUB-ID

Cite this

Campbell GRO, Sharypova LA, Scheidle H, et al. Striking complexity of lipopolysaccharide defects in a collection of Sinorhizobium meliloti mutants. Journal of Bacteriology. 2003;185(13):3853-3862.
Campbell, G. R. O., Sharypova, L. A., Scheidle, H., Jones, K. M., Niehaus, K., Becker, A., & Walker, G. C. (2003). Striking complexity of lipopolysaccharide defects in a collection of Sinorhizobium meliloti mutants. Journal of Bacteriology, 185(13), 3853-3862.
Campbell, G. R. O., Sharypova, L. A., Scheidle, H., Jones, K. M., Niehaus, K., Becker, A., and Walker, G. C. (2003). Striking complexity of lipopolysaccharide defects in a collection of Sinorhizobium meliloti mutants. Journal of Bacteriology 185, 3853-3862.
Campbell, G.R.O., et al., 2003. Striking complexity of lipopolysaccharide defects in a collection of Sinorhizobium meliloti mutants. Journal of Bacteriology, 185(13), p 3853-3862.
G.R.O. Campbell, et al., “Striking complexity of lipopolysaccharide defects in a collection of Sinorhizobium meliloti mutants”, Journal of Bacteriology, vol. 185, 2003, pp. 3853-3862.
Campbell, G.R.O., Sharypova, L.A., Scheidle, H., Jones, K.M., Niehaus, K., Becker, A., Walker, G.C.: Striking complexity of lipopolysaccharide defects in a collection of Sinorhizobium meliloti mutants. Journal of Bacteriology. 185, 3853-3862 (2003).
Campbell, G. R. O., Sharypova, L. A., Scheidle, H., Jones, K. M., Niehaus, Karsten, Becker, A., and Walker, G. C. “Striking complexity of lipopolysaccharide defects in a collection of Sinorhizobium meliloti mutants”. Journal of Bacteriology 185.13 (2003): 3853-3862.
This data publication is cited in the following publications:
This publication cites the following data publications:

38 Citations in Europe PMC

Data provided by Europe PubMed Central.

The Sinorhizobium meliloti EmrR regulator is required for efficient colonization of Medicago sativa root nodules.
Santos MR, Marques AT, Becker JD, Moreira LM., Mol. Plant Microbe Interact. 27(4), 2014
PMID: 24593245
The Sinorhizobium fredii HH103 lipopolysaccharide is not only relevant at early soybean nodulation stages but also for symbiosome stability in mature nodules.
Margaret I, Lucas MM, Acosta-Jurado S, Buendia-Claveria AM, Fedorova E, Hidalgo A, Rodriguez-Carvajal MA, Rodriguez-Navarro DN, Ruiz-Sainz JE, Vinardell JM., PLoS ONE 8(10), 2013
PMID: 24098345
The Sinorhizobium meliloti essential porin RopA1 is a target for numerous bacteriophages.
Crook MB, Draper AL, Guillory RJ, Griffitts JS., J. Bacteriol. 195(16), 2013
PMID: 23749981
Queuosine biosynthesis is required for sinorhizobium meliloti-induced cytoskeletal modifications on HeLa Cells and symbiosis with Medicago truncatula.
Marchetti M, Capela D, Poincloux R, Benmeradi N, Auriac MC, Le Ru A, Maridonneau-Parini I, Batut J, Masson-Boivin C., PLoS ONE 8(2), 2013
PMID: 23409119
Molecular insights into bacteroid development during Rhizobium-legume symbiosis.
Haag AF, Arnold MF, Myka KK, Kerscher B, Dall'Angelo S, Zanda M, Mergaert P, Ferguson GP., FEMS Microbiol. Rev. 37(3), 2013
PMID: 22998605
A comparative genomics screen identifies a Sinorhizobium meliloti 1021 sodM-like gene strongly expressed within host plant nodules.
Queiroux C, Washburn BK, Davis OM, Stewart J, Brewer TE, Lyons MR, Jones KM., BMC Microbiol. 12(), 2012
PMID: 22587634
Role for Rhizobium rhizogenes K84 cell envelope polysaccharides in surface interactions.
Abarca-Grau AM, Burbank LP, de Paz HD, Crespo-Rivas JC, Marco-Noales E, Lopez MM, Vinardell JM, von Bodman SB, Penyalver R., Appl. Environ. Microbiol. 78(6), 2012
PMID: 22210213
The incomplete substitution of lipopolysaccharide with O-chain prevents the establishment of effective symbiosis between Mesorhizobium loti NZP2213.1 and Lotus corniculatus.
Turska-Szewczuk A, Lotocka B, Kutkowska J, Krol J, Urbanik-Sypniewska T, Russa R., Microbiol. Res. 164(2), 2009
PMID: 17321732
Epitope tagging of legume root nodule extensin modifies protein structure and crosslinking in cell walls of transformed tobacco leaves.
Gucciardo S, Rathbun EA, Shanks M, Jenkyns S, Mak L, Durrant MC, Brewin NJ., Mol. Plant Microbe Interact. 18(1), 2005
PMID: 15672815

Export

0 Marked Publications

Open Data PUB

Web of Science

View record in Web of Science®

Sources

PMID: 12813079
PubMed | Europe PMC

Search this title in

Google Scholar