cDNA cloning of 12 subunits of the V-type ATPase from Mesembryanthemum crystallinum and their expression under stress

Kluge C, Lamkemeyer P, Tavakoli N, Golldack D, Kandlbinder A, Dietz K-J (2003)
MOLECULAR MEMBRANE BIOLOGY 20(2): 171-183.

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Abstract
The vacuolar-type ATPase (V-ATPase) and the vacuolar H+-pyrophosphatase are electrogenic proton pumps at plant endomembranes that create the proton motive force required for secondary activated transport and metabolite accumulation during development and adaptation to a variety of adverse growth conditions. Twelve distinct vacuolar H+-ATPase (VHA) subunits are suggested to constitute the functional V-ATPase complex. Starting from the available expressed sequence tag ( EST) sequences and by homology screening, the complete set of 12 VHA subunits was cloned as cDNAs from the halophyte Mesembryanthemum crystallinum, vha-A-H, - a, - c, - d and - e. Transcript levels of all 12 VHA subunits as well as of tonoplast pyrophosphatase and P-ATPase were analysed in root and leaf tissue under conditions of osmotic (700 mM mannitol), heat and cold stress, and salinity. Distinct coordinated changes of stress-induced expression were observed for most subunits in roots and leaves, with mostly paralleled changes in transcript levels of all subunits. In some cases, contrasting responses were seen for vha-B and - c transcript amounts.
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Kluge C, Lamkemeyer P, Tavakoli N, Golldack D, Kandlbinder A, Dietz K-J. cDNA cloning of 12 subunits of the V-type ATPase from Mesembryanthemum crystallinum and their expression under stress. MOLECULAR MEMBRANE BIOLOGY. 2003;20(2):171-183.
Kluge, C., Lamkemeyer, P., Tavakoli, N., Golldack, D., Kandlbinder, A., & Dietz, K. - J. (2003). cDNA cloning of 12 subunits of the V-type ATPase from Mesembryanthemum crystallinum and their expression under stress. MOLECULAR MEMBRANE BIOLOGY, 20(2), 171-183.
Kluge, C., Lamkemeyer, P., Tavakoli, N., Golldack, D., Kandlbinder, A., and Dietz, K. - J. (2003). cDNA cloning of 12 subunits of the V-type ATPase from Mesembryanthemum crystallinum and their expression under stress. MOLECULAR MEMBRANE BIOLOGY 20, 171-183.
Kluge, C., et al., 2003. cDNA cloning of 12 subunits of the V-type ATPase from Mesembryanthemum crystallinum and their expression under stress. MOLECULAR MEMBRANE BIOLOGY, 20(2), p 171-183.
C. Kluge, et al., “cDNA cloning of 12 subunits of the V-type ATPase from Mesembryanthemum crystallinum and their expression under stress”, MOLECULAR MEMBRANE BIOLOGY, vol. 20, 2003, pp. 171-183.
Kluge, C., Lamkemeyer, P., Tavakoli, N., Golldack, D., Kandlbinder, A., Dietz, K.-J.: cDNA cloning of 12 subunits of the V-type ATPase from Mesembryanthemum crystallinum and their expression under stress. MOLECULAR MEMBRANE BIOLOGY. 20, 171-183 (2003).
Kluge, C, Lamkemeyer, P, Tavakoli, N, Golldack, Dortje, Kandlbinder, Andrea, and Dietz, Karl-Josef. “cDNA cloning of 12 subunits of the V-type ATPase from Mesembryanthemum crystallinum and their expression under stress”. MOLECULAR MEMBRANE BIOLOGY 20.2 (2003): 171-183.
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Cosentino C, Di Silvestre D, Fischer-Schliebs E, Homann U, De Palma A, Comunian C, Mauri PL, Thiel G., Biochem. J. 450(2), 2013
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Tyagi W, Singla-Pareek S, Nair S, Reddy MK, Sopory SK., Plant Cell Rep. 25(2), 2006
PMID: 16404601
Transcript profiling of salinity stress responses by large-scale expressed sequence tag analysis in Mesembryanthemum crystallinum.
Kore-eda S, Cushman MA, Akselrod I, Bufford D, Fredrickson M, Clark E, Cushman JC., Gene 341(), 2004
PMID: 15474291
Subcellular distribution of the V-ATPase complex in plant cells, and in vivo localisation of the 100 kDa subunit VHA-a within the complex.
Kluge C, Seidel T, Bolte S, Sharma SS, Hanitzsch M, Satiat-Jeunemaitre B, Ross J, Sauer M, Golldack D, Dietz KJ., BMC Cell Biol. 5(), 2004
PMID: 15310389
Colocalization and FRET-analysis of subunits c and a of the vacuolar H+-ATPase in living plant cells.
Seidel T, Kluge C, Hanitzsch M, Ross J, Sauer M, Dietz KJ, Golldack D., J. Biotechnol. 112(1-2), 2004
PMID: 15288951

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