Cultivation of Dictyostelium discoideum in immobilized form by colonization of porous supports

Beshay U, Friehs K, Azzam AEM, Flaschel E (2003)
PROCESS BIOCHEMISTRY 38(11): 1521-1529.

Download
Es wurde kein Volltext hochgeladen. Nur Publikationsnachweis!
Zeitschriftenaufsatz | Veröffentlicht | Englisch
Autor
; ; ;
Abstract / Bemerkung
Dictyostelium discoideum is a social amoeba. It shows promising characteristics as an expression system for the production of recombinant glycoproteins owing to its ability to perform a whole spectrum of post-translational modifications. However, its application is seriously affected by slow growth rates as well as low maximal cell densities. Since the technique of immobilization often is applied in order to achieve high cell concentrations, D. discoideum was cultivated in the presence of different porous supports with the aim of studying its growth in an immobilized state. On a small scale a shake flask cultivation system with external loop was applied for this purpose. On a larger scale a bioreactor was coupled with an external loop of a column containing a fixed bed of the porous support. During growth on standard axenic (soluble) media, cell densities in the pores of the supports were attained, which were more than one magnitude higher than those normally obtained during suspension culture. Electron micrograph images indicate how D. discoideum colonizes porous support particles. (C) 2003 Elsevier Science Ltd. All rights reserved.
Erscheinungsjahr
Zeitschriftentitel
PROCESS BIOCHEMISTRY
Band
38
Zeitschriftennummer
11
Seite
1521-1529
ISSN
PUB-ID

Zitieren

Beshay U, Friehs K, Azzam AEM, Flaschel E. Cultivation of Dictyostelium discoideum in immobilized form by colonization of porous supports. PROCESS BIOCHEMISTRY. 2003;38(11):1521-1529.
Beshay, U., Friehs, K., Azzam, A. E. M., & Flaschel, E. (2003). Cultivation of Dictyostelium discoideum in immobilized form by colonization of porous supports. PROCESS BIOCHEMISTRY, 38(11), 1521-1529. doi:10.1016/S0032-9592(03)00042-6
Beshay, U., Friehs, K., Azzam, A. E. M., and Flaschel, E. (2003). Cultivation of Dictyostelium discoideum in immobilized form by colonization of porous supports. PROCESS BIOCHEMISTRY 38, 1521-1529.
Beshay, U., et al., 2003. Cultivation of Dictyostelium discoideum in immobilized form by colonization of porous supports. PROCESS BIOCHEMISTRY, 38(11), p 1521-1529.
U. Beshay, et al., “Cultivation of Dictyostelium discoideum in immobilized form by colonization of porous supports”, PROCESS BIOCHEMISTRY, vol. 38, 2003, pp. 1521-1529.
Beshay, U., Friehs, K., Azzam, A.E.M., Flaschel, E.: Cultivation of Dictyostelium discoideum in immobilized form by colonization of porous supports. PROCESS BIOCHEMISTRY. 38, 1521-1529 (2003).
Beshay, U, Friehs, Karl, Azzam, AEM, and Flaschel, Erwin. “Cultivation of Dictyostelium discoideum in immobilized form by colonization of porous supports”. PROCESS BIOCHEMISTRY 38.11 (2003): 1521-1529.