Genome-based analysis of biosynthetic aminotransferase genes of Corynebacterium glutamicum

McHardy AC, Tauch A, Rückert C, Pühler A, Kalinowski J (2003)
J Biotechnol 104(1-3): 229-240.

Journal Article | Published | English

No fulltext has been uploaded

Abstract
Due to broad and overlapping substrate specificities, aminotransferases remain the last uncharacterized enzymes from most amino acid biosynthetic pathways in Corynebacterium glutamicum. We report here a complete description of all aminotransferases participating in the biosynthesis of the branched-chain amino acids and phenylalanine in C glutamicum. We used methods of profile analysis on the newly available genome sequence to systematically search for and characterize members of the four known aminotransferase classes. This led to the discovery of sixteen new, potential aminotransferase encoding genes in the C glutamicum genome, eleven of which were subsequently characterized experimentally with respect to their participation in different amino acid biosynthetic pathways. Disruption by insertion mutagenesis of ilvE, encoding a branched-chain amino acid aminotransferase, confirmed its function in leucine and isoleucine biosynthesis. Two double mutants lacking both ilvE and genes classified as class I aminotransferases exhibited additional auxotrophic requirements for valine and phenylalanine, respectively. In C glutamicum the branched-chain amino acid aminotransferase thus participates in four amino acid biosynthetic pathways, for which in case of valine and phenylalanine biosynthesis two additional enzymes with overlapping substrate specificity exist. The novel protein with aminotransferase activity in valine biosynthesis belongs to the very recently described MocR subfamily of GntR-type helix-turn-helix transcriptional regulators, is located upstream of a potential operon of a newly described pyridoxine biosynthetic pathway and when disrupted, gives rise to a pyridoxine auxotrophy. The theoretical and experimental data we present should further provide a solid platform for ongoing research and understanding of the network of aminotransferases which participate in amino acid biosynthesis in C glutamicum. (C) 2003 Elsevier B.V. All rights reserved.
Publishing Year
ISSN
PUB-ID

Cite this

McHardy AC, Tauch A, Rückert C, Pühler A, Kalinowski J. Genome-based analysis of biosynthetic aminotransferase genes of Corynebacterium glutamicum. J Biotechnol. 2003;104(1-3):229-240.
McHardy, A. C., Tauch, A., Rückert, C., Pühler, A., & Kalinowski, J. (2003). Genome-based analysis of biosynthetic aminotransferase genes of Corynebacterium glutamicum. J Biotechnol, 104(1-3), 229-240.
McHardy, A. C., Tauch, A., Rückert, C., Pühler, A., and Kalinowski, J. (2003). Genome-based analysis of biosynthetic aminotransferase genes of Corynebacterium glutamicum. J Biotechnol 104, 229-240.
McHardy, A.C., et al., 2003. Genome-based analysis of biosynthetic aminotransferase genes of Corynebacterium glutamicum. J Biotechnol, 104(1-3), p 229-240.
A.C. McHardy, et al., “Genome-based analysis of biosynthetic aminotransferase genes of Corynebacterium glutamicum”, J Biotechnol, vol. 104, 2003, pp. 229-240.
McHardy, A.C., Tauch, A., Rückert, C., Pühler, A., Kalinowski, J.: Genome-based analysis of biosynthetic aminotransferase genes of Corynebacterium glutamicum. J Biotechnol. 104, 229-240 (2003).
McHardy, AC, Tauch, Andreas, Rückert, Christian, Pühler, Alfred, and Kalinowski, Jörn. “Genome-based analysis of biosynthetic aminotransferase genes of Corynebacterium glutamicum”. J Biotechnol 104.1-3 (2003): 229-240.
This data publication is cited in the following publications:
This publication cites the following data publications:

14 Citations in Europe PMC

Data provided by Europe PubMed Central.

Histidine biosynthesis, its regulation and biotechnological application in Corynebacterium glutamicum.
Kulis-Horn RK, Persicke M, Kalinowski J., Microb Biotechnol 7(1), 2014
PMID: 23617600
Factors enhancing L-valine production by the growth-limited L-isoleucine auxotrophic strain Corynebacterium glutamicum DeltailvA DeltapanB ilvNM13 (pECKAilvBNC).
Denina I, Paegle L, Prouza M, Holatko J, Patek M, Nesvera J, Ruklisha M., J. Ind. Microbiol. Biotechnol. 37(7), 2010
PMID: 20364396
Identification of novel L-amino acid alpha-ligases through Hidden Markov Model-based profile analysis.
Senoo A, Tabata K, Yonetani Y, Yagasaki M., Biosci. Biotechnol. Biochem. 74(2), 2010
PMID: 20139602
Genetic makeup of the Corynebacterium glutamicum LexA regulon deduced from comparative transcriptomics and in vitro DNA band shift assays.
Jochmann N, Kurze AK, Czaja LF, Brinkrolf K, Brune I, Huser AT, Hansmeier N, Puhler A, Borovok I, Tauch A., Microbiology (Reading, Engl.) 155(Pt 5), 2009
PMID: 19372162
Towards systems metabolic engineering of microorganisms for amino acid production.
Park JH, Lee SY., Curr. Opin. Biotechnol. 19(5), 2008
PMID: 18760356
The IclR-type transcriptional repressor LtbR regulates the expression of leucine and tryptophan biosynthesis genes in the amino acid producer Corynebacterium glutamicum.
Brune I, Jochmann N, Brinkrolf K, Huser AT, Gerstmeir R, Eikmanns BJ, Kalinowski J, Puhler A, Tauch A., J. Bacteriol. 189(7), 2007
PMID: 17259312
The transcriptional regulatory network of the amino acid producer Corynebacterium glutamicum.
Brinkrolf K, Brune I, Tauch A., J. Biotechnol. 129(2), 2007
PMID: 17227685
Emerging Corynebacterium glutamicum systems biology.
Wendisch VF, Bott M, Kalinowski J, Oldiges M, Wiechert W., J. Biotechnol. 124(1), 2006
PMID: 16406159
Functional analysis of all aminotransferase proteins inferred from the genome sequence of Corynebacterium glutamicum.
Marienhagen J, Kennerknecht N, Sahm H, Eggeling L., J. Bacteriol. 187(22), 2005
PMID: 16267288
Rational design of a Corynebacterium glutamicum pantothenate production strain and its characterization by metabolic flux analysis and genome-wide transcriptional profiling.
Huser AT, Chassagnole C, Lindley ND, Merkamm M, Guyonvarch A, Elisakova V, Patek M, Kalinowski J, Brune I, Puhler A, Tauch A., Appl. Environ. Microbiol. 71(6), 2005
PMID: 15933028
The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of L-aspartate-derived amino acids and vitamins.
Kalinowski J, Bathe B, Bartels D, Bischoff N, Bott M, Burkovski A, Dusch N, Eggeling L, Eikmanns BJ, Gaigalat L, Goesmann A, Hartmann M, Huthmacher K, Kramer R, Linke B, McHardy AC, Meyer F, Mockel B, Pfefferle W, Puhler A, Rey DA, Ruckert C, Rupp O, Sahm H, Wendisch VF, Wiegrabe I, Tauch A., J. Biotechnol. 104(1-3), 2003
PMID: 12948626

Export

0 Marked Publications

Open Data PUB

Web of Science

View record in Web of Science®

Sources

PMID: 12948641
PubMed | Europe PMC

Search this title in

Google Scholar