Proteomics of Chlamydomonas reinhardtii light-harvesting proteins

Stauber EJ, Fink A, Markert C, Kruse O, Johanningmeier U, Hippler M (2003)
EUKARYOTIC CELL 2(5): 978-994.

Download
Es wurde kein Volltext hochgeladen. Nur Publikationsnachweis!
Zeitschriftenaufsatz | Veröffentlicht | Englisch
Autor
; ; ; ; ;
Abstract / Bemerkung
With the recent development of techniques for analyzing transmembrane thylakoid proteins by two-dimensional gel electrophoresis, systematic approaches for proteomic analyses of membrane proteins became feasible. In this study, we established detailed two-dimensional protein maps of Chlamydomonas reinhardtii light-harvesting proteins (Lhca and Lhcb) by extensive tandem mass spectrometric analysis. We predicted eight distinct Lhcb proteins. Although the major Lheb proteins were highly similar, we identified peptides which were unique for specific lhcbm gene products. Interestingly, 1hcbm6 gene products were resolved as multiple spots with different masses and isoelectric points. Gene tagging experiments confirmed the presence of differentially N-terminally processed Lhcbm6 proteins. The mass spectrometric data also revealed differentially N-terminally processed forms of Lhcbm3 and phosphorylation of a threonine residue in the N terminus. The N-terminal processing of Lhcbm3 leads to the removal of the phosphorylation site, indicating a potential novel regulatory mechanism. At least nine different lhca-related gene products were predicted by comparison of the mass spectrometric data against Chlamydomonas expressed sequence tag and genomic databases, demonstrating the extensive variability of the C. reinhardtii Lhca antenna system. Out of these nine, three were identified for the first time at the protein level. This proteomic study demonstrates the complexity of the light-harvesting proteins at the protein level in C. reinhardtii and will be an important basis of future functional studies addressing this diversity.
Erscheinungsjahr
Zeitschriftentitel
EUKARYOTIC CELL
Band
2
Zeitschriftennummer
5
Seite
978-994
ISSN
eISSN
PUB-ID

Zitieren

Stauber EJ, Fink A, Markert C, Kruse O, Johanningmeier U, Hippler M. Proteomics of Chlamydomonas reinhardtii light-harvesting proteins. EUKARYOTIC CELL. 2003;2(5):978-994.
Stauber, E. J., Fink, A., Markert, C., Kruse, O., Johanningmeier, U., & Hippler, M. (2003). Proteomics of Chlamydomonas reinhardtii light-harvesting proteins. EUKARYOTIC CELL, 2(5), 978-994. doi:10.1128/EC.2.5.978-994.2003
Stauber, E. J., Fink, A., Markert, C., Kruse, O., Johanningmeier, U., and Hippler, M. (2003). Proteomics of Chlamydomonas reinhardtii light-harvesting proteins. EUKARYOTIC CELL 2, 978-994.
Stauber, E.J., et al., 2003. Proteomics of Chlamydomonas reinhardtii light-harvesting proteins. EUKARYOTIC CELL, 2(5), p 978-994.
E.J. Stauber, et al., “Proteomics of Chlamydomonas reinhardtii light-harvesting proteins”, EUKARYOTIC CELL, vol. 2, 2003, pp. 978-994.
Stauber, E.J., Fink, A., Markert, C., Kruse, O., Johanningmeier, U., Hippler, M.: Proteomics of Chlamydomonas reinhardtii light-harvesting proteins. EUKARYOTIC CELL. 2, 978-994 (2003).
Stauber, EJ, Fink, A, Markert, C, Kruse, Olaf, Johanningmeier, U, and Hippler, M. “Proteomics of Chlamydomonas reinhardtii light-harvesting proteins”. EUKARYOTIC CELL 2.5 (2003): 978-994.

66 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Single particle electron microscopy in combination with mass spectrometry to investigate novel complexes of membrane proteins.
Arteni AA, Nowaczyk M, Lax J, Kouril R, Rögner M, Boekema EJ., J Struct Biol 149(3), 2005
PMID: 15721586
New insights into the nature and phylogeny of prasinophyte antenna proteins: Ostreococcus tauri, a case study.
Six C, Worden AZ, Rodríguez F, Moreau H, Partensky F., Mol Biol Evol 22(11), 2005
PMID: 16049197
Light-harvesting complex II protein CP29 binds to photosystem I of Chlamydomonas reinhardtii under State 2 conditions.
Kargul J, Turkina MV, Nield J, Benson S, Vener AV, Barber J., FEBS J 272(18), 2005
PMID: 16156798
Spectral and kinetic analysis of the energy coupling in the PS I-LHC I supercomplex from the green alga Chlamydomonas reinhardtii at 77 K.
Melkozernov AN, Kargul J, Lin S, Barber J, Blankenship RE., Photosynth Res 86(1-2), 2005
PMID: 16172939
Structure, function and assembly of Photosystem II and its light-harvesting proteins.
Minagawa J, Takahashi Y., Photosynth Res 82(3), 2004
PMID: 16143838
Functional proteomics of circadian expressed proteins from Chlamydomonas reinhardtii.
Wagner V, Fiedler M, Markert C, Hippler M, Mittag M., FEBS Lett 559(1-3), 2004
PMID: 14960320
Chloroplast proteomics: potentials and challenges.
Baginsky S, Gruissem W., J Exp Bot 55(400), 2004
PMID: 15020636
Successful herbivore attack due to metabolic diversion of a plant chemical defense.
Wittstock U, Agerbirk N, Stauber EJ, Olsen CE, Hippler M, Mitchell-Olds T, Gershenzon J, Vogel H., Proc Natl Acad Sci U S A 101(14), 2004
PMID: 15051878
Chlamydomonas reinhardtii proteomics.
Stauber EJ, Hippler M., Plant Physiol Biochem 42(12), 2004
PMID: 15707836

47 References

Daten bereitgestellt von Europe PubMed Central.

Stable nuclear transformation of Chlamydomonas using the Chlamydomonas gene for nitrate reductase.
Kindle KL, Schnell RA, Fernandez E, Lefebvre PA., J. Cell Biol. 109(6 Pt 1), 1989
PMID: 2592399
Atomic model of plant light-harvesting complex by electron crystallography.
Kuhlbrandt W, Wang DN, Fujiyoshi Y., Nature 367(6464), 1994
PMID: 8107845

AUTHOR UNKNOWN, 1989
Adaptation to Fe-deficiency requires remodeling of the photosynthetic apparatus.
Moseley JL, Allinger T, Herzog S, Hoerth P, Wehinger E, Merchant S, Hippler M., EMBO J. 21(24), 2002
PMID: 12485992
The tomato Cab-4 and Cab-5 genes encode a second type of CAB polypeptides localized in Photosystem II.
Pichersky E, Hoffman NE, Malik VS, Bernatzky R, Tanksley SD, Szabo L, Cashmore AR., Plant Mol. Biol. 9(2), 1987
PMID: IND92000041

AUTHOR UNKNOWN, 1989
A chloroplast processing enzyme functions as the general stromal processing peptidase.
Richter S, Lamppa GK., Proc. Natl. Acad. Sci. U.S.A. 95(13), 1998
PMID: 9636172

AUTHOR UNKNOWN, 1989
Membrane proteins and proteomics: un amour impossible?
Santoni V, Molloy M, Rabilloud T., Electrophoresis 21(6), 2000
PMID: 10786880
Challenges and prospects of plant proteomics.
van Wijk KJ., Plant Physiol. 126(2), 2001
PMID: 11402181

AUTHOR UNKNOWN, 2002

Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®

Quellen

PMID: 14555480
PubMed | Europe PMC

Suchen in

Google Scholar