Kinetic analysis of the binding of hemopexin-like domain of gelatinase B cloned and expressed in Pichia pastoris to tissue inhibitor of metalloproteinases-1

Stute J, Pourmotabbed T, Tschesche H (2003)
JOURNAL OF PROTEIN CHEMISTRY 22(6): 509-514.

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Abstract
The gelatinases are a subgroup of the matrix metalloproteinase family. The interaction of their C-terminal hemopexin-like domain with a tissue inhibitor of metalloproteinases (TIMP) is a major part of the regulatory mechanisms of gelatinases. To investigate the interaction of the hemopexin-like domain of gelatinase B (92-Pex) and TIMP-1, we expressed the individual domain in Pichia pastoris. The active refolded domain was purified by ion exchange chromatography and gel filtration. We investigated the formation of the 92-Pex/TIMP-1 complex by surface plasmon resonance (SPR). The dissociation constant K-d was calculated to be 0.86 nM. Analogous to the complex of the hemopexin-like domain of gelatinase A and TIMP-2 (Olson, M. W. et al., 1997), the binding curves of the 92-Pex/TIMP-1 complex were best fitted with a monophasic model.
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Stute J, Pourmotabbed T, Tschesche H. Kinetic analysis of the binding of hemopexin-like domain of gelatinase B cloned and expressed in Pichia pastoris to tissue inhibitor of metalloproteinases-1. JOURNAL OF PROTEIN CHEMISTRY. 2003;22(6):509-514.
Stute, J., Pourmotabbed, T., & Tschesche, H. (2003). Kinetic analysis of the binding of hemopexin-like domain of gelatinase B cloned and expressed in Pichia pastoris to tissue inhibitor of metalloproteinases-1. JOURNAL OF PROTEIN CHEMISTRY, 22(6), 509-514.
Stute, J., Pourmotabbed, T., and Tschesche, H. (2003). Kinetic analysis of the binding of hemopexin-like domain of gelatinase B cloned and expressed in Pichia pastoris to tissue inhibitor of metalloproteinases-1. JOURNAL OF PROTEIN CHEMISTRY 22, 509-514.
Stute, J., Pourmotabbed, T., & Tschesche, H., 2003. Kinetic analysis of the binding of hemopexin-like domain of gelatinase B cloned and expressed in Pichia pastoris to tissue inhibitor of metalloproteinases-1. JOURNAL OF PROTEIN CHEMISTRY, 22(6), p 509-514.
J. Stute, T. Pourmotabbed, and H. Tschesche, “Kinetic analysis of the binding of hemopexin-like domain of gelatinase B cloned and expressed in Pichia pastoris to tissue inhibitor of metalloproteinases-1”, JOURNAL OF PROTEIN CHEMISTRY, vol. 22, 2003, pp. 509-514.
Stute, J., Pourmotabbed, T., Tschesche, H.: Kinetic analysis of the binding of hemopexin-like domain of gelatinase B cloned and expressed in Pichia pastoris to tissue inhibitor of metalloproteinases-1. JOURNAL OF PROTEIN CHEMISTRY. 22, 509-514 (2003).
Stute, J, Pourmotabbed, T, and Tschesche, Harald. “Kinetic analysis of the binding of hemopexin-like domain of gelatinase B cloned and expressed in Pichia pastoris to tissue inhibitor of metalloproteinases-1”. JOURNAL OF PROTEIN CHEMISTRY 22.6 (2003): 509-514.
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