Influence of medium composition on growth behaviour of Dictyostelium discoideum for cultivation on axenic media

Stephan M, Beshay U, Friehs K, Flaschel E (2003)
PROCESS BIOCHEMISTRY 39(3): 333-343.

Journal Article | Published | English

No fulltext has been uploaded

Author
; ; ;
Abstract
The social amoeba Dictyostelium discoideum represents an attractive host organism for the production of heterologous proteins. However, its application is seriously affected by slow growth rates as well as low maximal cell densities in the presence of axenic (liquid) media. Starting with standard complex media the influence of certain medium components was investigated. The kind and concentration of carbohydrates, the concentration of salt and ammonia and the supplementation of conditioned media were varied. These studies were performed by following cell growth in batch experiments over the whole growth cycle into the decline phase to obtain information about growth rates as well as maximal cell densities. Only maltose, glucose and a-trehalose were metabolised at significant rates. The concentration of ammonia produced correlated inversely with the concentration of carbohydrates metabolised. Under standard conditions ammonia reached concentrations of about 50 mM, most being produced during stationary phase. Ionic strength and the addition of ammonia affected the growth rate as well as the maximal cell density. Ammonia did not limit maximal cell density. D. discoideum can be cultivated in normal stirred bioreactors. A semi-empirical model is discussed for the description of the growth behaviour. (C) 2003 Elsevier Science Ltd. All rights reserved.
Publishing Year
ISSN
PUB-ID

Cite this

Stephan M, Beshay U, Friehs K, Flaschel E. Influence of medium composition on growth behaviour of Dictyostelium discoideum for cultivation on axenic media. PROCESS BIOCHEMISTRY. 2003;39(3):333-343.
Stephan, M., Beshay, U., Friehs, K., & Flaschel, E. (2003). Influence of medium composition on growth behaviour of Dictyostelium discoideum for cultivation on axenic media. PROCESS BIOCHEMISTRY, 39(3), 333-343.
Stephan, M., Beshay, U., Friehs, K., and Flaschel, E. (2003). Influence of medium composition on growth behaviour of Dictyostelium discoideum for cultivation on axenic media. PROCESS BIOCHEMISTRY 39, 333-343.
Stephan, M., et al., 2003. Influence of medium composition on growth behaviour of Dictyostelium discoideum for cultivation on axenic media. PROCESS BIOCHEMISTRY, 39(3), p 333-343.
M. Stephan, et al., “Influence of medium composition on growth behaviour of Dictyostelium discoideum for cultivation on axenic media”, PROCESS BIOCHEMISTRY, vol. 39, 2003, pp. 333-343.
Stephan, M., Beshay, U., Friehs, K., Flaschel, E.: Influence of medium composition on growth behaviour of Dictyostelium discoideum for cultivation on axenic media. PROCESS BIOCHEMISTRY. 39, 333-343 (2003).
Stephan, M, Beshay, U, Friehs, Karl, and Flaschel, Erwin. “Influence of medium composition on growth behaviour of Dictyostelium discoideum for cultivation on axenic media”. PROCESS BIOCHEMISTRY 39.3 (2003): 333-343.
This data publication is cited in the following publications:
This publication cites the following data publications:

Export

0 Marked Publications

Open Data PUB

Web of Science

View record in Web of Science®

Search this title in

Google Scholar