Qualitative and quantitative proteomics by two-dimensional gel electrophoresis, peptide mass fingerprint and a chemically-coded affinity tag (CCAT)

Watt SA, Patschkowski T, Kalinowski J, Niehaus K (2003)
Journal of Biotechnology 106(2-3): 287-300.

Download
Es wurde kein Volltext hochgeladen. Nur Publikationsnachweis!
Zeitschriftenaufsatz | Veröffentlicht | Englisch
Abstract / Bemerkung
The chemically-coded affinity tag (CCAT) method combines standard electrophoresis protocols with MALDI-TOF-MS analysis to identify and quantify protein abundances in complex samples in one step. This method is designed to fit into the workflow of SDS-PAGE or two-dimensional electrophoresis (2-DE) only requiring basic proteome laboratory equipment. Prior to electrophoresis two protein samples are separately labelled with a heavy or a light version of the CCAT reagent via reduced cysteines in the proteins. Equal amounts are then combined and electrophoretically separated. Proteins can then be excised from the gel to obtain their peptide mass fingerprint by mass spectrometry. This fingerprint enabled not only identification, but also quantification by comparing relative peak intensities of CCAT-labelled peptides. In this article, we display how the CCAT method can be used to analyse two protein samples in one gel and that the peak intensities of labelled peptides reflect the abundance of a protein in it. (C) 2003 Published by Elsevier B.V.
Erscheinungsjahr
Zeitschriftentitel
Journal of Biotechnology
Band
106
Zeitschriftennummer
2-3
Seite
287-300
ISSN
PUB-ID

Zitieren

Watt SA, Patschkowski T, Kalinowski J, Niehaus K. Qualitative and quantitative proteomics by two-dimensional gel electrophoresis, peptide mass fingerprint and a chemically-coded affinity tag (CCAT). Journal of Biotechnology. 2003;106(2-3):287-300.
Watt, S. A., Patschkowski, T., Kalinowski, J., & Niehaus, K. (2003). Qualitative and quantitative proteomics by two-dimensional gel electrophoresis, peptide mass fingerprint and a chemically-coded affinity tag (CCAT). Journal of Biotechnology, 106(2-3), 287-300. doi:10.1016/j.jbiotec.2003.07.014
Watt, S. A., Patschkowski, T., Kalinowski, J., and Niehaus, K. (2003). Qualitative and quantitative proteomics by two-dimensional gel electrophoresis, peptide mass fingerprint and a chemically-coded affinity tag (CCAT). Journal of Biotechnology 106, 287-300.
Watt, S.A., et al., 2003. Qualitative and quantitative proteomics by two-dimensional gel electrophoresis, peptide mass fingerprint and a chemically-coded affinity tag (CCAT). Journal of Biotechnology, 106(2-3), p 287-300.
S.A. Watt, et al., “Qualitative and quantitative proteomics by two-dimensional gel electrophoresis, peptide mass fingerprint and a chemically-coded affinity tag (CCAT)”, Journal of Biotechnology, vol. 106, 2003, pp. 287-300.
Watt, S.A., Patschkowski, T., Kalinowski, J., Niehaus, K.: Qualitative and quantitative proteomics by two-dimensional gel electrophoresis, peptide mass fingerprint and a chemically-coded affinity tag (CCAT). Journal of Biotechnology. 106, 287-300 (2003).
Watt, S. A., Patschkowski, Thomas, Kalinowski, Jörn, and Niehaus, Karsten. “Qualitative and quantitative proteomics by two-dimensional gel electrophoresis, peptide mass fingerprint and a chemically-coded affinity tag (CCAT)”. Journal of Biotechnology 106.2-3 (2003): 287-300.

5 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Systems and synthetic biology perspective of the versatile plant-pathogenic and polysaccharide-producing bacterium Xanthomonas campestris.
Schatschneider S, Schneider J, Blom J, Létisse F, Niehaus K, Goesmann A, Vorhölter FJ., Microbiology 163(8), 2017
PMID: 28795660
Understanding the blood-brain barrier using gene and protein expression profiling technologies.
Pottiez G, Flahaut C, Cecchelli R, Karamanos Y., Brain Res Rev 62(1), 2009
PMID: 19770003
Mass spectrometry-based peptide quantification: applications and limitations.
Pütz S, Reinders J, Reinders Y, Sickmann A., Expert Rev Proteomics 2(3), 2005
PMID: 16000084
State-of-the-art in phosphoproteomics.
Reinders J, Sickmann A., Proteomics 5(16), 2005
PMID: 16196093

Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®

Quellen

PMID: 14651868
PubMed | Europe PMC

Suchen in

Google Scholar