Whole genome shotgun sequencing guided-by bioinformatics pipelines - An optimized approach for an established technique

Kaiser O, Bartels D, Bekel T, Goesmann A, Kespohl S, Pühler A, Meyer F (2003)
JOURNAL OF BIOTECHNOLOGY 106(2-3): 121-133.

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Abstract
While the sequencing of bacterial genomes has become a routine procedure at major sequencing centers, there are still a number of genome projects at small- or medium-size facilities. For these facilities a maximum of control over sequencing, assembling and finishing is essential. At the same time, facilities have to be able to co-operate at minimum costs for the overall project. We have established a pipeline for the distributed sequencing of Alcanivorax borkumensis SK2, Azoarcus sp. BH72, Clavibacter michiganensis subsp. michiganensis NCPPB382, Sorangium cellulosum So ce56 and Xanthomonas campestris pv. vesicatoria 85-10. Our pipeline relies on standard tools (e.g. PHRED/PHRAP, CAP3 and Consed/Autofinish) wherever possible, supplementing them with new tools (BioMake and BACCardI) to achieve the aims described above. (C) 2003 Elsevier B.V. All rights reserved.
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Kaiser O, Bartels D, Bekel T, et al. Whole genome shotgun sequencing guided-by bioinformatics pipelines - An optimized approach for an established technique. JOURNAL OF BIOTECHNOLOGY. 2003;106(2-3):121-133.
Kaiser, O., Bartels, D., Bekel, T., Goesmann, A., Kespohl, S., Pühler, A., & Meyer, F. (2003). Whole genome shotgun sequencing guided-by bioinformatics pipelines - An optimized approach for an established technique. JOURNAL OF BIOTECHNOLOGY, 106(2-3), 121-133.
Kaiser, O., Bartels, D., Bekel, T., Goesmann, A., Kespohl, S., Pühler, A., and Meyer, F. (2003). Whole genome shotgun sequencing guided-by bioinformatics pipelines - An optimized approach for an established technique. JOURNAL OF BIOTECHNOLOGY 106, 121-133.
Kaiser, O., et al., 2003. Whole genome shotgun sequencing guided-by bioinformatics pipelines - An optimized approach for an established technique. JOURNAL OF BIOTECHNOLOGY, 106(2-3), p 121-133.
O. Kaiser, et al., “Whole genome shotgun sequencing guided-by bioinformatics pipelines - An optimized approach for an established technique”, JOURNAL OF BIOTECHNOLOGY, vol. 106, 2003, pp. 121-133.
Kaiser, O., Bartels, D., Bekel, T., Goesmann, A., Kespohl, S., Pühler, A., Meyer, F.: Whole genome shotgun sequencing guided-by bioinformatics pipelines - An optimized approach for an established technique. JOURNAL OF BIOTECHNOLOGY. 106, 121-133 (2003).
Kaiser, O., Bartels, D., Bekel, Thomas, Goesmann, Alexander, Kespohl, S., Pühler, Alfred, and Meyer, F. “Whole genome shotgun sequencing guided-by bioinformatics pipelines - An optimized approach for an established technique”. JOURNAL OF BIOTECHNOLOGY 106.2-3 (2003): 121-133.
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13 Citations in Europe PMC

Data provided by Europe PubMed Central.

Whole-genome sequencing and gene mapping of a newly isolated lytic enterococcal bacteriophage EFRM31.
Fard RM, Barton MD, Arthur JL, Heuzenroeder MW., Arch. Virol. 155(11), 2010
PMID: 20844906
The genome of Xanthomonas campestris pv. campestris B100 and its use for the reconstruction of metabolic pathways involved in xanthan biosynthesis.
Vorholter FJ, Schneiker S, Goesmann A, Krause L, Bekel T, Kaiser O, Linke B, Patschkowski T, Ruckert C, Schmid J, Sidhu VK, Sieber V, Tauch A, Watt SA, Weisshaar B, Becker A, Niehaus K, Puhler A., J. Biotechnol. 134(1-2), 2008
PMID: 18304669
The genome sequence of the tomato-pathogenic actinomycete Clavibacter michiganensis subsp. michiganensis NCPPB382 reveals a large island involved in pathogenicity.
Gartemann KH, Abt B, Bekel T, Burger A, Engemann J, Flugel M, Gaigalat L, Goesmann A, Grafen I, Kalinowski J, Kaup O, Kirchner O, Krause L, Linke B, McHardy A, Meyer F, Pohle S, Ruckert C, Schneiker S, Zellermann EM, Puhler A, Eichenlaub R, Kaiser O, Bartels D., J. Bacteriol. 190(6), 2008
PMID: 18192381
Complete genome sequence of the myxobacterium Sorangium cellulosum.
Schneiker S, Perlova O, Kaiser O, Gerth K, Alici A, Altmeyer MO, Bartels D, Bekel T, Beyer S, Bode E, Bode HB, Bolten CJ, Choudhuri JV, Doss S, Elnakady YA, Frank B, Gaigalat L, Goesmann A, Groeger C, Gross F, Jelsbak L, Jelsbak L, Kalinowski J, Kegler C, Knauber T, Konietzny S, Kopp M, Krause L, Krug D, Linke B, Mahmud T, Martinez-Arias R, McHardy AC, Merai M, Meyer F, Mormann S, Munoz-Dorado J, Perez J, Pradella S, Rachid S, Raddatz G, Rosenau F, Ruckert C, Sasse F, Scharfe M, Schuster SC, Suen G, Treuner-Lange A, Velicer GJ, Vorholter FJ, Weissman KJ, Welch RD, Wenzel SC, Whitworth DE, Wilhelm S, Wittmann C, Blocker H, Puhler A, Muller R., Nat. Biotechnol. 25(11), 2007
PMID: 17965706
Complete genome of the mutualistic, N2-fixing grass endophyte Azoarcus sp. strain BH72.
Krause A, Ramakumar A, Bartels D, Battistoni F, Bekel T, Boch J, Bohm M, Friedrich F, Hurek T, Krause L, Linke B, McHardy AC, Sarkar A, Schneiker S, Syed AA, Thauer R, Vorholter FJ, Weidner S, Puhler A, Reinhold-Hurek B, Kaiser O, Goesmann A., Nat. Biotechnol. 24(11), 2006
PMID: 17057704
Genome sequence of the ubiquitous hydrocarbon-degrading marine bacterium Alcanivorax borkumensis.
Schneiker S, Martins dos Santos VA, Bartels D, Bekel T, Brecht M, Buhrmester J, Chernikova TN, Denaro R, Ferrer M, Gertler C, Goesmann A, Golyshina OV, Kaminski F, Khachane AN, Lang S, Linke B, McHardy AC, Meyer F, Nechitaylo T, Puhler A, Regenhardt D, Rupp O, Sabirova JS, Selbitschka W, Yakimov MM, Timmis KN, Vorholter FJ, Weidner S, Kaiser O, Golyshin PN., Nat. Biotechnol. 24(8), 2006
PMID: 16878126
The crystal structure of hypothetical methyltransferase from Thermus thermophilus HB8.
Sasaki C, Sugiura I, Ebihara A, Tamura T, Sugio S, Inagaki K., Proteins 64(2), 2006
PMID: 16700050
Insights into genome plasticity and pathogenicity of the plant pathogenic bacterium Xanthomonas campestris pv. vesicatoria revealed by the complete genome sequence.
Thieme F, Koebnik R, Bekel T, Berger C, Boch J, Buttner D, Caldana C, Gaigalat L, Goesmann A, Kay S, Kirchner O, Lanz C, Linke B, McHardy AC, Meyer F, Mittenhuber G, Nies DH, Niesbach-Klosgen U, Patschkowski T, Ruckert C, Rupp O, Schneiker S, Schuster SC, Vorholter FJ, Weber E, Puhler A, Bonas U, Bartels D, Kaiser O., J. Bacteriol. 187(21), 2005
PMID: 16237009
Shotgun sequencing of the negative-sense RNA genome of the rhabdovirus Maize mosaic virus.
Reed SE, Tsai CW, Willie KJ, Redinbaugh MG, Hogenhout SA., J. Virol. Methods 129(1), 2005
PMID: 16005085
Complete genome sequence and analysis of the multiresistant nosocomial pathogen Corynebacterium jeikeium K411, a lipid-requiring bacterium of the human skin flora.
Tauch A, Kaiser O, Hain T, Goesmann A, Weisshaar B, Albersmeier A, Bekel T, Bischoff N, Brune I, Chakraborty T, Kalinowski J, Meyer F, Rupp O, Schneiker S, Viehoever P, Puhler A., J. Bacteriol. 187(13), 2005
PMID: 15968079
BACCardI--a tool for the validation of genomic assemblies, assisting genome finishing and intergenome comparison.
Bartels D, Kespohl S, Albaum S, Druke T, Goesmann A, Herold J, Kaiser O, Puhler A, Pfeiffer F, Raddatz G, Stoye J, Meyer F, Schuster SC., Bioinformatics 21(7), 2005
PMID: 15514001

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