Cultivation of Dictyostelium discoideum on an improved synthetic medium in a conventional bioreactor

Han SI, Friehs K, Flaschel E (2004)
PROCESS BIOCHEMISTRY 39(5): 585-589.

Journal Article | Published | English

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Abstract
An improved fully synthetic medium was presented recently [Process Biochem. (in press)] based on the synthetic medium formulated by Franke and Kessin in 1977 [Proc. Natl. Acad. Sci. USA 74 (1977) 2157]. This novel medium was called SIH instead of the classical FM medium. The improved medium leads to even higher cell densities, a more balanced uptake of amino acids and a better utilisation of glucose. However, the growth behaviour had only been assessed during shake-flask cultivation. Here it is shown that Dictyostelium discoideum can be cultivated in conventional stirred tank-type bioreactors, if the operating conditions take care about the shear sensitivity of the cells. A batch as well as a fed-batch cultivation show that mass cultivation of D. discoideum can be achieved during growth on synthetic media. (C) 2003 Elsevier Ltd. All rights reserved.
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Han SI, Friehs K, Flaschel E. Cultivation of Dictyostelium discoideum on an improved synthetic medium in a conventional bioreactor. PROCESS BIOCHEMISTRY. 2004;39(5):585-589.
Han, S. I., Friehs, K., & Flaschel, E. (2004). Cultivation of Dictyostelium discoideum on an improved synthetic medium in a conventional bioreactor. PROCESS BIOCHEMISTRY, 39(5), 585-589.
Han, S. I., Friehs, K., and Flaschel, E. (2004). Cultivation of Dictyostelium discoideum on an improved synthetic medium in a conventional bioreactor. PROCESS BIOCHEMISTRY 39, 585-589.
Han, S.I., Friehs, K., & Flaschel, E., 2004. Cultivation of Dictyostelium discoideum on an improved synthetic medium in a conventional bioreactor. PROCESS BIOCHEMISTRY, 39(5), p 585-589.
S.I. Han, K. Friehs, and E. Flaschel, “Cultivation of Dictyostelium discoideum on an improved synthetic medium in a conventional bioreactor”, PROCESS BIOCHEMISTRY, vol. 39, 2004, pp. 585-589.
Han, S.I., Friehs, K., Flaschel, E.: Cultivation of Dictyostelium discoideum on an improved synthetic medium in a conventional bioreactor. PROCESS BIOCHEMISTRY. 39, 585-589 (2004).
Han, SI, Friehs, Karl, and Flaschel, Erwin. “Cultivation of Dictyostelium discoideum on an improved synthetic medium in a conventional bioreactor”. PROCESS BIOCHEMISTRY 39.5 (2004): 585-589.
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