Cultivation of Dictyostelium discoideum on an improved synthetic medium in a conventional bioreactor

Han SI, Friehs K, Flaschel E (2004)
PROCESS BIOCHEMISTRY 39(5): 585-589.

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Zeitschriftenaufsatz | Veröffentlicht | Englisch
Abstract / Bemerkung
An improved fully synthetic medium was presented recently [Process Biochem. (in press)] based on the synthetic medium formulated by Franke and Kessin in 1977 [Proc. Natl. Acad. Sci. USA 74 (1977) 2157]. This novel medium was called SIH instead of the classical FM medium. The improved medium leads to even higher cell densities, a more balanced uptake of amino acids and a better utilisation of glucose. However, the growth behaviour had only been assessed during shake-flask cultivation. Here it is shown that Dictyostelium discoideum can be cultivated in conventional stirred tank-type bioreactors, if the operating conditions take care about the shear sensitivity of the cells. A batch as well as a fed-batch cultivation show that mass cultivation of D. discoideum can be achieved during growth on synthetic media. (C) 2003 Elsevier Ltd. All rights reserved.
Erscheinungsjahr
Zeitschriftentitel
PROCESS BIOCHEMISTRY
Band
39
Zeitschriftennummer
5
Seite
585-589
ISSN
PUB-ID

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Han SI, Friehs K, Flaschel E. Cultivation of Dictyostelium discoideum on an improved synthetic medium in a conventional bioreactor. PROCESS BIOCHEMISTRY. 2004;39(5):585-589.
Han, S. I., Friehs, K., & Flaschel, E. (2004). Cultivation of Dictyostelium discoideum on an improved synthetic medium in a conventional bioreactor. PROCESS BIOCHEMISTRY, 39(5), 585-589. doi:10.1016/S0032-9592(03)00122-5
Han, S. I., Friehs, K., and Flaschel, E. (2004). Cultivation of Dictyostelium discoideum on an improved synthetic medium in a conventional bioreactor. PROCESS BIOCHEMISTRY 39, 585-589.
Han, S.I., Friehs, K., & Flaschel, E., 2004. Cultivation of Dictyostelium discoideum on an improved synthetic medium in a conventional bioreactor. PROCESS BIOCHEMISTRY, 39(5), p 585-589.
S.I. Han, K. Friehs, and E. Flaschel, “Cultivation of Dictyostelium discoideum on an improved synthetic medium in a conventional bioreactor”, PROCESS BIOCHEMISTRY, vol. 39, 2004, pp. 585-589.
Han, S.I., Friehs, K., Flaschel, E.: Cultivation of Dictyostelium discoideum on an improved synthetic medium in a conventional bioreactor. PROCESS BIOCHEMISTRY. 39, 585-589 (2004).
Han, SI, Friehs, Karl, and Flaschel, Erwin. “Cultivation of Dictyostelium discoideum on an improved synthetic medium in a conventional bioreactor”. PROCESS BIOCHEMISTRY 39.5 (2004): 585-589.