Production of the soluble human Fas ligand by Dictyostelium discoideum cultivated on a synthetic medium

Lu YH, Knol JC, Linskens MHK, Friehs K, van Haastert PJM, Flaschel E (2004)
JOURNAL OF BIOTECHNOLOGY 108(3): 243-251.

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Abstract
Human Fas ligand (hFasL) is of considerable interest since it is a type II transmembrane glycoprotein that induces programmed cell death, or apoptosis. In this study Dictyostelium discoideum was used to produce a soluble form of the human Fas ligand. The recombinant cells were adapted to a modified synthetic FM medium, called SIH medium. Cells adapted to the SIH medium reached about 2 times higher cell densities and hFasL concentrations on this medium compared with cells growing on the standard complex medium HL-5C. Even higher values were achieved by a dissolved oxygen-controlled fed-batch cultivation in a conventional stirred bioreactor on SIH medium. Cell densities of up to 5.5 x 10(7) ml(-1) and a maximum hFasL concentration of 148 mug l (1) were obtained. These results were further improved by means of continuous cultivation of D. discoideum in a bioreactor equipped with cell retention by microfiltration. At low space velocity very high cell densities of up to 2.4 x 10(8) ml(-1) and hFasL concentrations of up to 205 mug l(-1) were achieved. (C) 2004 Elsevier B.V. All rights reserved.
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Lu YH, Knol JC, Linskens MHK, Friehs K, van Haastert PJM, Flaschel E. Production of the soluble human Fas ligand by Dictyostelium discoideum cultivated on a synthetic medium. JOURNAL OF BIOTECHNOLOGY. 2004;108(3):243-251.
Lu, Y. H., Knol, J. C., Linskens, M. H. K., Friehs, K., van Haastert, P. J. M., & Flaschel, E. (2004). Production of the soluble human Fas ligand by Dictyostelium discoideum cultivated on a synthetic medium. JOURNAL OF BIOTECHNOLOGY, 108(3), 243-251.
Lu, Y. H., Knol, J. C., Linskens, M. H. K., Friehs, K., van Haastert, P. J. M., and Flaschel, E. (2004). Production of the soluble human Fas ligand by Dictyostelium discoideum cultivated on a synthetic medium. JOURNAL OF BIOTECHNOLOGY 108, 243-251.
Lu, Y.H., et al., 2004. Production of the soluble human Fas ligand by Dictyostelium discoideum cultivated on a synthetic medium. JOURNAL OF BIOTECHNOLOGY, 108(3), p 243-251.
Y.H. Lu, et al., “Production of the soluble human Fas ligand by Dictyostelium discoideum cultivated on a synthetic medium”, JOURNAL OF BIOTECHNOLOGY, vol. 108, 2004, pp. 243-251.
Lu, Y.H., Knol, J.C., Linskens, M.H.K., Friehs, K., van Haastert, P.J.M., Flaschel, E.: Production of the soluble human Fas ligand by Dictyostelium discoideum cultivated on a synthetic medium. JOURNAL OF BIOTECHNOLOGY. 108, 243-251 (2004).
Lu, YH, Knol, JC, Linskens, MHK, Friehs, Karl, van Haastert, PJM, and Flaschel, Erwin. “Production of the soluble human Fas ligand by Dictyostelium discoideum cultivated on a synthetic medium”. JOURNAL OF BIOTECHNOLOGY 108.3 (2004): 243-251.
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