A Rab-related small GTP binding protein is predominantly expressed in root nodules of Medicago sativa

Schiene K, Donath S, Brecht M, Pühler A, Niehaus K (2004)
Molecular Genetics and Genomics 272(1): 57-66.

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Abstract
Rab-related small GTP-binding proteins are known to be involved in the regulation of the vesicular transport system in eucaryotic cells. In this paper we report the isolation of the cDNA clone MS- rab11f from Medicago sativa (alfalfa) root nodules using a combination of RT-PCR and SSCP analysis. MS- rab11f shows high homology to the Rab-related cDNA clone LJ- rab11f from Lotus japonicus root nodules. The MS-Rab11F protein expressed in Escherichia coli was found to bind GTP, confirming that the isolated cDNA indeed codes for a small GTP-binding protein. Expression analysis by RT-PCR demonstrated that MS- rab11f is preferentially expressed in root nodules of alfalfa. Using the cDNA-sequence of MS-rab11f, a peptide-specific antibody was generated. Western blot analysis with this antibody revealed that two Rab11F isoforms, designated MS-Rab11FA and MS-Rab11FB, are found in M. sativa root nodules.
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Schiene K, Donath S, Brecht M, Pühler A, Niehaus K. A Rab-related small GTP binding protein is predominantly expressed in root nodules of Medicago sativa. Molecular Genetics and Genomics. 2004;272(1):57-66.
Schiene, K., Donath, S., Brecht, M., Pühler, A., & Niehaus, K. (2004). A Rab-related small GTP binding protein is predominantly expressed in root nodules of Medicago sativa. Molecular Genetics and Genomics, 272(1), 57-66.
Schiene, K., Donath, S., Brecht, M., Pühler, A., and Niehaus, K. (2004). A Rab-related small GTP binding protein is predominantly expressed in root nodules of Medicago sativa. Molecular Genetics and Genomics 272, 57-66.
Schiene, K., et al., 2004. A Rab-related small GTP binding protein is predominantly expressed in root nodules of Medicago sativa. Molecular Genetics and Genomics, 272(1), p 57-66.
K. Schiene, et al., “A Rab-related small GTP binding protein is predominantly expressed in root nodules of Medicago sativa”, Molecular Genetics and Genomics, vol. 272, 2004, pp. 57-66.
Schiene, K., Donath, S., Brecht, M., Pühler, A., Niehaus, K.: A Rab-related small GTP binding protein is predominantly expressed in root nodules of Medicago sativa. Molecular Genetics and Genomics. 272, 57-66 (2004).
Schiene, K., Donath, S., Brecht, M., Pühler, Alfred, and Niehaus, Karsten. “A Rab-related small GTP binding protein is predominantly expressed in root nodules of Medicago sativa”. Molecular Genetics and Genomics 272.1 (2004): 57-66.
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