Identification of mycorrhiza-regulated genes with arbuscule development-related expression profile

Grunwald U, Nyamsuren O, Tarnasloukht M, Lapopin L, Becker A, Mann P, Gianinazzi-Pearson V, Krajinski F, Franken P (2004)
PLANT MOLECULAR BIOLOGY 55(4): 553-566.

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Abstract
Suppressive subtractive hybridisation was applied to the analysis of late stage arbuscular mycorrhizal development in pea. 96 cDNA clones were amplified and 8 1, which carried fragments more than 200 nt in size, were sequence analysed. Among 67 unique fragments, 10 showed no homology and 10 were similar to sequences with unknown function. RNA accumulation of the corresponding 67 genes was analysed by hybridisation of macro-arrays. The cDNAs used as probes were derived from roots of wild type and late mutant pea genotypes, inoculated or not with the AM fungus Glomus mosseae. After calibration, a more than 2.5-fold mycorrhiza-induced RNA accumulation was detected in two independent experiments in the wild type for 25 genes, 22 of which seemed to be induced specifically during late stage AM development. Differential expression for 7 genes was confirmed by RT-PCR using RNA from mycorrhiza and from controls of a different pea cultivar. In order to confirm arbuscule-related expression, the Medicago truncatula EST data base was screened for homologous sequences with putative mycorrhiza-related expression and among a number of sequences with significant similarities, a family of trypsin inhibitor genes could be identified. Mycorrhiza-induced RNA accumulation was verified for five members by real-time PCR and arbuscule-related activation of the promoter could be shown in transgenic roots for one of the genes, MtTi1.
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Grunwald U, Nyamsuren O, Tarnasloukht M, et al. Identification of mycorrhiza-regulated genes with arbuscule development-related expression profile. PLANT MOLECULAR BIOLOGY. 2004;55(4):553-566.
Grunwald, U., Nyamsuren, O., Tarnasloukht, M., Lapopin, L., Becker, A., Mann, P., Gianinazzi-Pearson, V., et al. (2004). Identification of mycorrhiza-regulated genes with arbuscule development-related expression profile. PLANT MOLECULAR BIOLOGY, 55(4), 553-566.
Grunwald, U., Nyamsuren, O., Tarnasloukht, M., Lapopin, L., Becker, A., Mann, P., Gianinazzi-Pearson, V., Krajinski, F., and Franken, P. (2004). Identification of mycorrhiza-regulated genes with arbuscule development-related expression profile. PLANT MOLECULAR BIOLOGY 55, 553-566.
Grunwald, U., et al., 2004. Identification of mycorrhiza-regulated genes with arbuscule development-related expression profile. PLANT MOLECULAR BIOLOGY, 55(4), p 553-566.
U. Grunwald, et al., “Identification of mycorrhiza-regulated genes with arbuscule development-related expression profile”, PLANT MOLECULAR BIOLOGY, vol. 55, 2004, pp. 553-566.
Grunwald, U., Nyamsuren, O., Tarnasloukht, M., Lapopin, L., Becker, A., Mann, P., Gianinazzi-Pearson, V., Krajinski, F., Franken, P.: Identification of mycorrhiza-regulated genes with arbuscule development-related expression profile. PLANT MOLECULAR BIOLOGY. 55, 553-566 (2004).
Grunwald, U., Nyamsuren, O., Tarnasloukht, M., Lapopin, L., Becker, Anke, Mann, P., Gianinazzi-Pearson, V., Krajinski, F., and Franken, P. “Identification of mycorrhiza-regulated genes with arbuscule development-related expression profile”. PLANT MOLECULAR BIOLOGY 55.4 (2004): 553-566.
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86 References

Data provided by Europe PubMed Central.

Structural diversity in (vesicular)-arbuscular mycorrhizal symbioses.
Smith FA, Smith SE., New Phytol. 137(3), 1997
PMID: IND20799742
Molecular cloning and expression of a MAP kinase homologue from pea.
Stafstrom JP, Altschuler M, Anderson DH., Plant Mol. Biol. 22(1), 1993
PMID: 8388749

Staehelin, P Natl. Acad. Sci. USA 98(), 2001

Strittmatter, Mol. Plant-Microbe Interact 9(), 1996
New plant promoter and enhancer testing vectors.
Szabados L, Charrier B, Kondorosi A, Bruijn FJde, Ratet P., Mol. Breed. 1(4), 1995
PMID: IND21967687

Timonen, Plant Soil 244(), 2002
Protein phosphatase 2A holoenzyme and its subunits from Medicago sativa.
Toth EC, Vissi E, Kovacs I, Szoke A, Arino J, Gergely P, Dudits D, Dombradi V., Plant Mol. Biol. 43(4), 2000
PMID: 11052204

Trouvelot, 1986

Van, Mol. Plant-Microbe Interact 12(), 1999

Wulf, Mol. Plant-Microbe Interact 16(), 2003

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