Heat shock Proteome analysis of wild-type Corynebacterium glutamicum ATCC 13032 and a spontaneous mutant lacking GroEL1, a dispensable chaperone

Barreiro C, Gonzalez-Lavado E, Brand S, Tauch A, Martin JF (2005)
JOURNAL OF BACTERIOLOGY 187(3): 884-889.

Journal Article | Published | English

No fulltext has been uploaded

Author
; ; ; ;
Abstract
Proteome analysis of Corynebacterium glutanticum ATCC 13032 showed that levels of several proteins increased drastically in response to heat shock. These proteins were identified as DnaK, GroEL1, GroEL2, ClpB, GrpE, and PoxB, and their heat response was in agreement with previous transcriptomic results. A major heat-induced protein was absent in the proteome of strain 13032B of C. glutamicum, used for genome sequencing in Germany, compared with the wild-type ATCC 13032 strain. The missing protein was identified as GroEL1 by matrix-assisted laser desorption ionization-time of flight peptide mass fingerprinting, and the mutation was found to be due to an insertion sequence, IsCg1, that was integrated at position 327 downstream of the translation start codon of the groEL1 gene, resulting in a truncated transcript of this gene, as shown by Northern analysis. The GroEL1 chaperone is, therefore, dispensable in C. glutamicum. On the other hand, GroEL2 appears to be essential for growth. Based on these results, the role of the duplicate groEL1 and groEL2 genes is analyzed.
Publishing Year
ISSN
PUB-ID

Cite this

Barreiro C, Gonzalez-Lavado E, Brand S, Tauch A, Martin JF. Heat shock Proteome analysis of wild-type Corynebacterium glutamicum ATCC 13032 and a spontaneous mutant lacking GroEL1, a dispensable chaperone. JOURNAL OF BACTERIOLOGY. 2005;187(3):884-889.
Barreiro, C., Gonzalez-Lavado, E., Brand, S., Tauch, A., & Martin, J. F. (2005). Heat shock Proteome analysis of wild-type Corynebacterium glutamicum ATCC 13032 and a spontaneous mutant lacking GroEL1, a dispensable chaperone. JOURNAL OF BACTERIOLOGY, 187(3), 884-889.
Barreiro, C., Gonzalez-Lavado, E., Brand, S., Tauch, A., and Martin, J. F. (2005). Heat shock Proteome analysis of wild-type Corynebacterium glutamicum ATCC 13032 and a spontaneous mutant lacking GroEL1, a dispensable chaperone. JOURNAL OF BACTERIOLOGY 187, 884-889.
Barreiro, C., et al., 2005. Heat shock Proteome analysis of wild-type Corynebacterium glutamicum ATCC 13032 and a spontaneous mutant lacking GroEL1, a dispensable chaperone. JOURNAL OF BACTERIOLOGY, 187(3), p 884-889.
C. Barreiro, et al., “Heat shock Proteome analysis of wild-type Corynebacterium glutamicum ATCC 13032 and a spontaneous mutant lacking GroEL1, a dispensable chaperone”, JOURNAL OF BACTERIOLOGY, vol. 187, 2005, pp. 884-889.
Barreiro, C., Gonzalez-Lavado, E., Brand, S., Tauch, A., Martin, J.F.: Heat shock Proteome analysis of wild-type Corynebacterium glutamicum ATCC 13032 and a spontaneous mutant lacking GroEL1, a dispensable chaperone. JOURNAL OF BACTERIOLOGY. 187, 884-889 (2005).
Barreiro, C, Gonzalez-Lavado, E, Brand, S, Tauch, Andreas, and Martin, JF. “Heat shock Proteome analysis of wild-type Corynebacterium glutamicum ATCC 13032 and a spontaneous mutant lacking GroEL1, a dispensable chaperone”. JOURNAL OF BACTERIOLOGY 187.3 (2005): 884-889.
This data publication is cited in the following publications:
This publication cites the following data publications:

30 Citations in Europe PMC

Data provided by Europe PubMed Central.

Multiple chaperonins in bacteria--novel functions and non-canonical behaviors.
Kumar CM, Mande SC, Mahajan G., Cell Stress Chaperones 20(4), 2015
PMID: 25986150
Proteome response of Corynebacterium glutamicum to high concentration of industrially relevant C₄ and C₅ dicarboxylic acids.
Vasco-Cardenas MF, Banos S, Ramos A, Martin JF, Barreiro C., J Proteomics 85(), 2013
PMID: 23624027
Crystallization and preliminary X-ray crystallographic analysis of a GroEL1 fragment from Mycobacterium tuberculosis H37Rv.
Sielaff B, Lee KS, Tsai FT., Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 66(Pt 4), 2010
PMID: 20383012

41 References

Data provided by Europe PubMed Central.


AUTHOR UNKNOWN, 1999

AUTHOR UNKNOWN, 1987
Transcriptome and proteome analysis of Bacillus subtilis gene expression in response to superoxide and peroxide stress.
Mostertz J, Scharf C, Hecker M, Homuth G., Microbiology (Reading, Engl.) 150(Pt 2), 2004
PMID: 14766928
Genome-wide transcription profiling of Corynebacterium glutamicum after heat shock and during growth on acetate and glucose.
Muffler A, Bettermann S, Haushalter M, Horlein A, Neveling U, Schramm M, Sorgenfrei O., J. Biotechnol. 98(2-3), 2002
PMID: 12141991
Efficient 40 degrees C fermentation of L-lysine by a new Corynebacterium glutamicum mutant developed by genome breeding.
Ohnishi J, Hayashi M, Mitsuhashi S, Ikeda M., Appl. Microbiol. Biotechnol. 62(1), 2003
PMID: 12835923
Probability-based protein identification by searching sequence databases using mass spectrometry data.
Perkins DN, Pappin DJ, Creasy DM, Cottrell JS., Electrophoresis 20(18), 1999
PMID: 10612281
Proteome analysis in the study of the bacterial heat-shock response.
Rosen R, Ron EZ., Mass Spectrom Rev 21(4), 2002
PMID: 12533799

AUTHOR UNKNOWN, 2001
A high-resolution reference map for cytoplasmic and membrane-associated proteins of Corynebacterium glutamicum.
Schaffer S, Weil B, Nguyen VD, Dongmann G, Gunther K, Nickolaus M, Hermann T, Bott M., Electrophoresis 22(20), 2001
PMID: 11824608

AUTHOR UNKNOWN, 1980
Isolation and characterization of IS31831, a transposable element from Corynebacterium glutamicum.
Vertes AA, Inui M, Kobayashi M, Kurusu Y, Yukawa H., Mol. Microbiol. 11(4), 1994
PMID: 8196545
Heat shock proteins and antigens of Mycobacterium tuberculosis.
Young DB, Garbe TR., Infect. Immun. 59(9), 1991
PMID: 1679042
Regulation of the heat-shock response.
Yura T, Nakahigashi K., Curr. Opin. Microbiol. 2(2), 1999
PMID: 10322172

Export

0 Marked Publications

Open Data PUB

Web of Science

View record in Web of Science®

Sources

PMID: 15659666
PubMed | Europe PMC

Search this title in

Google Scholar