Comprehensive analysis of the extracellular proteins from Xanthomonas campestris pv. campestris B100

Watt SA, Wilke A, Patschkowski T, Niehaus K (2005)
Proteomics 5(1): 153-167.

Download
Es wurde kein Volltext hochgeladen. Nur Publikationsnachweis!
Zeitschriftenaufsatz | Veröffentlicht | Englisch
Autor
Abstract / Bemerkung
The extracellular proteome of Xanthomonas campestris pv. campestris (Xcc) cultivated in minimal medium was isolated from the cell-free culture supernatant and separated by two-dimensional gel electrophoresis. This technique resolved 97 clearly visible protein spots, which were excised, digested with trypsin and identified on the basis of their peptide mass fingerprints generated by matrix assisted laser desorption/ionisation-time of flight-mass spectrometry. Using this approach 87 different proteins could be distinguished. The Signal P software predicted putative signal peptides for 53% of the extracellular proteins. These proteins are probably transported over the inner membrane and are localized in the periplasm, the outer membrane or secreted into the extracellular space. Among the secreted proteins are 11 degradative enzymes, which are involved in pathogenesis of Xcc. The proteins without obvious secretion signals are known to serve functions in the cytosol. How the cytosolic proteins are delivered to the extracellular space remains unclear.
Erscheinungsjahr
Zeitschriftentitel
Proteomics
Band
5
Zeitschriftennummer
1
Seite
153-167
ISSN
eISSN
PUB-ID

Zitieren

Watt SA, Wilke A, Patschkowski T, Niehaus K. Comprehensive analysis of the extracellular proteins from Xanthomonas campestris pv. campestris B100. Proteomics. 2005;5(1):153-167.
Watt, S. A., Wilke, A., Patschkowski, T., & Niehaus, K. (2005). Comprehensive analysis of the extracellular proteins from Xanthomonas campestris pv. campestris B100. Proteomics, 5(1), 153-167. doi:10.1002/pmic.200400905
Watt, S. A., Wilke, A., Patschkowski, T., and Niehaus, K. (2005). Comprehensive analysis of the extracellular proteins from Xanthomonas campestris pv. campestris B100. Proteomics 5, 153-167.
Watt, S.A., et al., 2005. Comprehensive analysis of the extracellular proteins from Xanthomonas campestris pv. campestris B100. Proteomics, 5(1), p 153-167.
S.A. Watt, et al., “Comprehensive analysis of the extracellular proteins from Xanthomonas campestris pv. campestris B100”, Proteomics, vol. 5, 2005, pp. 153-167.
Watt, S.A., Wilke, A., Patschkowski, T., Niehaus, K.: Comprehensive analysis of the extracellular proteins from Xanthomonas campestris pv. campestris B100. Proteomics. 5, 153-167 (2005).
Watt, S. A., Wilke, A., Patschkowski, Thomas, and Niehaus, Karsten. “Comprehensive analysis of the extracellular proteins from Xanthomonas campestris pv. campestris B100”. Proteomics 5.1 (2005): 153-167.

51 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Bacterial phytopathogens and genome science.
Setubal JC, Moreira LM, da Silva AC., Curr Opin Microbiol 8(5), 2005
PMID: 16125997

34 References

Daten bereitgestellt von Europe PubMed Central.


Gough, Mol. Plant-Microbe Interact. 1(), 1988

Tang, Mol. Gen. Genet. 210(), 1987

Dow, Physiol. Mol. Plant Pathol. 31(), 1987
Purification of a metalloprotease produced by Erwinia carotovora ssp. carotovora and the degradation of potato lectin in vitro.
Heilbronn J, Johnston DJ, Dunbar B, Lyon GD., Physiol. Mol. Plant Pathol. 47(5), 1995
PMID: IND20502586

Antelmann, Mol. Mocrobiol. 49(), 2003
Proteome analysis of the plant pathogen Xylella fastidiosa reveals major cellular and extracellular proteins and a peculiar codon bias distribution.
Smolka MB, Martins-de-Souza D, Martins D, Winck FV, Santoro CE, Castellari RR, Ferrari F, Brum IJ, Galembeck E, Della Coletta Filho H, Machado MA, Marangoni S, Novello JC., Proteomics 3(2), 2003
PMID: 12601815

Glasgow, 1998

Kjeldahl, 1883

Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®

Quellen

PMID: 15619296
PubMed | Europe PMC

Suchen in

Google Scholar