Sequence of the 68,869 bp IncP-1 alpha plasmid pTB11 from a waste-water treatment plant reveals a highly conserved backbone, a Tn402-like integron and other transposable elements

Tennstedt T, Szczepanowski R, Krahn I, Pühler A, Schlüter A (2005)
PLASMID 53(3): 218-238.

Download
No fulltext has been uploaded. References only!
Journal Article | Original Article | Published | English

No fulltext has been uploaded

Abstract
To analyse the significance of conjugative broad-host-range IncP-lot plasmids for the spread of antibiotic resistance determinants in waste-water treatment plants we isolated and characterised five different IncP-lot plasmids from bacteria of activated sludge and the final effluents of a municipal waste-water treatment plant. These plasmids mediate resistance to ampicillin, cefaclor, cefuroxime, gentamicin, kanamycin, spectinomycin, streptomycin, tetracycline, tobramycin, and trimethoprim. The complete 68,869 bp DNA-sequence of the IncP-1α plasmid pTB11 was determined. The pTB11 backbone modules for replication (Rep), mating pair formation (Trb), multimer resolution (Mrs), post-segregational killing (Psk), conjugative DNA-transfer (Tra), plasmid control (01), and stable maintenance and inheritance (KilA, KilE, and KilC) are highly conserved as compared to the ′ Birmingham′ IncP-lα plasmids. In contrast to the ′ Birmingham′ plasmids pTB11 carries an insert of a Tn402-derivative integrating a class 1 integron in the intergenic region between the multimer resolution operon parCBA and the post-segregational killing operon parDE. The integron comprises the resistance gene cassettes oxa2 (β-lactamase), aacA4 (aminoglycoside-6′ N-acetyltransferase), and aadA1 (aminoglycoside-3′-adenylyltransfetase) and a complete tniABQR transposition module. Integron-specific sequences were also identified on other IncP-lα plasmids analysed in this work. In contrast to the ′ Birmingham′ plasmids the pTB11 tetracycline resistance module carries a pecM- and a pncA-like gene downstream of the tetracycline resistance gene tetA and contains an insertion of the new insertion sequence element ISTB11. The transposable elements IS21 and Tn1 which disrupted, respectively, orf7 and klcB on the ′ Birmingham′ plasmids are not present on pTB11. Identification of IncP-lα plasmids in bacteria of the waste-water treatment plant's final effluents indicates that bacteria carrying these kind of plasmids are released into the environment. © 2004 Elsevier Inc. All rights reserved.
Publishing Year
ISSN
PUB-ID

Cite this

Tennstedt T, Szczepanowski R, Krahn I, Pühler A, Schlüter A. Sequence of the 68,869 bp IncP-1 alpha plasmid pTB11 from a waste-water treatment plant reveals a highly conserved backbone, a Tn402-like integron and other transposable elements. PLASMID. 2005;53(3):218-238.
Tennstedt, T., Szczepanowski, R., Krahn, I., Pühler, A., & Schlüter, A. (2005). Sequence of the 68,869 bp IncP-1 alpha plasmid pTB11 from a waste-water treatment plant reveals a highly conserved backbone, a Tn402-like integron and other transposable elements. PLASMID, 53(3), 218-238. doi:10.1016/j.plasmid.2004.09.004
Tennstedt, T., Szczepanowski, R., Krahn, I., Pühler, A., and Schlüter, A. (2005). Sequence of the 68,869 bp IncP-1 alpha plasmid pTB11 from a waste-water treatment plant reveals a highly conserved backbone, a Tn402-like integron and other transposable elements. PLASMID 53, 218-238.
Tennstedt, T., et al., 2005. Sequence of the 68,869 bp IncP-1 alpha plasmid pTB11 from a waste-water treatment plant reveals a highly conserved backbone, a Tn402-like integron and other transposable elements. PLASMID, 53(3), p 218-238.
T. Tennstedt, et al., “Sequence of the 68,869 bp IncP-1 alpha plasmid pTB11 from a waste-water treatment plant reveals a highly conserved backbone, a Tn402-like integron and other transposable elements”, PLASMID, vol. 53, 2005, pp. 218-238.
Tennstedt, T., Szczepanowski, R., Krahn, I., Pühler, A., Schlüter, A.: Sequence of the 68,869 bp IncP-1 alpha plasmid pTB11 from a waste-water treatment plant reveals a highly conserved backbone, a Tn402-like integron and other transposable elements. PLASMID. 53, 218-238 (2005).
Tennstedt, T., Szczepanowski, Rafael, Krahn, Irene, Pühler, Alfred, and Schlüter, Andreas. “Sequence of the 68,869 bp IncP-1 alpha plasmid pTB11 from a waste-water treatment plant reveals a highly conserved backbone, a Tn402-like integron and other transposable elements”. PLASMID 53.3 (2005): 218-238.
This data publication is cited in the following publications:
This publication cites the following data publications:

51 Citations in Europe PMC

Data provided by Europe PubMed Central.

Genomics of high molecular weight plasmids isolated from an on-farm biopurification system.
Martini MC, Wibberg D, Lozano M, Torres Tejerizo G, Albicoro FJ, Jaenicke S, van Elsas JD, Petroni A, Garcillan-Barcia MP, de la Cruz F, Schluter A, Puhler A, Pistorio M, Lagares A, Del Papa MF., Sci Rep 6(), 2016
PMID: 27321040

Export

0 Marked Publications

Open Data PUB

Web of Science

View record in Web of Science®

Sources

PMID: 15848226
PubMed | Europe PMC

Search this title in

Google Scholar