Plasmid pB8 is closely related to the prototype IncP-1 beta plasmid R751 but transfers poorly to Escherichia coli and carries a new transposon encoding a small multidrug resistance efflux protein

Schlüter A, Heuer H, Szczepanowski R, Poler SM, Schneiker-Bekel S, Pühler A, Top EM (2005)
PLASMID 54(2): 135-148.

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Zeitschriftenaufsatz | Veröffentlicht | Englisch
Abstract / Bemerkung
The IncP-1 beta plasmid pB8, which confers resistance to amoxicillin, spectinomycin, streptomycin, and sulfonamides, was previously isolated from a sewage treatment plant. It was found to possess abnormal conjugative transfer properties, i.e., transfer to Escherichia coli by conjugation or electroporation could not be detected. We showed in this study that plasmid pB8 is transferable to E coli by conjugation, but only at low frequencies and under specific experimental conditions, a phenomenon that is very unusual for IncP-1 plasmids. Determination of the complete 57,198 bp pB8 nucleotide sequence revealed that the backbone of the plasmid consists of a complete set of IncP-1 beta-specific genes for replication initiation, conjugative plasmid transfer, stable inheritance, and plasmid control with an organisation identical to that of the prototype IncP-1 beta plasmid R751. All of the minor differences in the pB8 backbone sequence compared to that of R751 were also found in other IncP-1 beta plasmids known to transfer to and replicate in E. coli. Plasmids pB8 and R751 can be distinguished with respect to their accessory genetic elements. First, the pB8 region downstream of the replication initiation gene trfA contains two transposable elements one of which is similar to Tn5501. The latter transposon encodes a putative post-segregational-killing system and the small multidrug resistance (SMR) protein QacF, mediating quaternary ammonium compound resistance. The accessory genes in this region are not responsible for the poor plasmid transfer to E coli since a pB8 deletion derivative devoid of all genes in that region showed the same conjugative transfer properties as pB8. A Tn5090/Tn402 derivative carrying a class 1 integron is located between the conjugative transfer modules. The Tn5090/Tn402 integration-sites are exactly identical on pB8 and R751 but in contrast to R751 the pB8 element carries the resistance gene cassettes oxa-2 for amoxicillin resistance and aadA4 for streptomycin/spectinomycin resistance, the integron-specific conserved segment consisting of the genes qacE Delta 1, sul1, and orf5, and a truncated tni transposition module (tniAB). Although future work will have to determine the molecular basis for the poor transfer of pB8 to E coli, our findings demonstrate that the host-range of typical IncP-1 plasmids may be less broad than expected. (c) 2005 Elsevier Inc. All rights reserved.
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PLASMID
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54
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2
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135-148
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Schlüter A, Heuer H, Szczepanowski R, et al. Plasmid pB8 is closely related to the prototype IncP-1 beta plasmid R751 but transfers poorly to Escherichia coli and carries a new transposon encoding a small multidrug resistance efflux protein. PLASMID. 2005;54(2):135-148.
Schlüter, A., Heuer, H., Szczepanowski, R., Poler, S. M., Schneiker-Bekel, S., Pühler, A., & Top, E. M. (2005). Plasmid pB8 is closely related to the prototype IncP-1 beta plasmid R751 but transfers poorly to Escherichia coli and carries a new transposon encoding a small multidrug resistance efflux protein. PLASMID, 54(2), 135-148. doi:10.1016/j.plasmid.2005.03.001
Schlüter, A., Heuer, H., Szczepanowski, R., Poler, S. M., Schneiker-Bekel, S., Pühler, A., and Top, E. M. (2005). Plasmid pB8 is closely related to the prototype IncP-1 beta plasmid R751 but transfers poorly to Escherichia coli and carries a new transposon encoding a small multidrug resistance efflux protein. PLASMID 54, 135-148.
Schlüter, A., et al., 2005. Plasmid pB8 is closely related to the prototype IncP-1 beta plasmid R751 but transfers poorly to Escherichia coli and carries a new transposon encoding a small multidrug resistance efflux protein. PLASMID, 54(2), p 135-148.
A. Schlüter, et al., “Plasmid pB8 is closely related to the prototype IncP-1 beta plasmid R751 but transfers poorly to Escherichia coli and carries a new transposon encoding a small multidrug resistance efflux protein”, PLASMID, vol. 54, 2005, pp. 135-148.
Schlüter, A., Heuer, H., Szczepanowski, R., Poler, S.M., Schneiker-Bekel, S., Pühler, A., Top, E.M.: Plasmid pB8 is closely related to the prototype IncP-1 beta plasmid R751 but transfers poorly to Escherichia coli and carries a new transposon encoding a small multidrug resistance efflux protein. PLASMID. 54, 135-148 (2005).
Schlüter, Andreas, Heuer, H., Szczepanowski, Rafael, Poler, S. M., Schneiker-Bekel, Susanne, Pühler, Alfred, and Top, E. M. “Plasmid pB8 is closely related to the prototype IncP-1 beta plasmid R751 but transfers poorly to Escherichia coli and carries a new transposon encoding a small multidrug resistance efflux protein”. PLASMID 54.2 (2005): 135-148.

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Complete nucleotide sequence and organization of the atrazine catabolic plasmid pADP-1 from Pseudomonas sp. strain ADP.
Martinez B, Tomkins J, Wackett LP, Wing R, Sadowsky MJ., J. Bacteriol. 183(19), 2001
PMID: 11544232
GenDB--an open source genome annotation system for prokaryote genomes.
Meyer F, Goesmann A, McHardy AC, Bartels D, Bekel T, Clausen J, Kalinowski J, Linke B, Rupp O, Giegerich R, Puhler A., Nucleic Acids Res. 31(8), 2003
PMID: 12682369
The 3' conserved segment of integrons contains a gene associated with multidrug resistance to antiseptics and disinfectants.
Paulsen IT, Littlejohn TG, Radstrom P, Sundstrom L, Skold O, Swedberg G, Skurray RA., Antimicrob. Agents Chemother. 37(4), 1993
PMID: 8494372
Characterization of In40 of Enterobacter aerogenes BM2688, a class 1 integron with two new gene cassettes, cmlA2 and qacF.
Ploy MC, Courvalin P, Lambert T., Antimicrob. Agents Chemother. 42(10), 1998
PMID: 9756755
Transposon Tn5090 of plasmid R751, which carries an integron, is related to Tn7, Mu, and the retroelements.
Radstrom P, Skold O, Swedberg G, Flensburg J, Roy PH, Sundstrom L., J. Bacteriol. 176(11), 1994
PMID: 8195081

Sambrook, 1989
Narrow-host-range IncP plasmid pHH502-1 lacks a complete IncP replication system.
Smith CA, Thomas CM., J. Gen. Microbiol. 133(8), 1987
PMID: 2832520

Thomas, 2000
Incompatibility group P plasmids: genetics, evolution, and use in genetic manipulation
Thomas, Ann. Rev. Microbiol. 41(), 1987
Complete sequence of the IncPbeta plasmid R751: implications for evolution and organisation of the IncP backbone.
Thorsted PB, Macartney DP, Akhtar P, Haines AS, Ali N, Davidson P, Stafford T, Pocklington MJ, Pansegrau W, Wilkins BM, Lanka E, Thomas CM., J. Mol. Biol. 282(5), 1998
PMID: 9753548
Determination of the mechanism of retrotransfer by mechanistic mathematical modeling.
Top E, Vanrolleghem P, Mergeay M, Verstraete W., J. Bacteriol. 174(18), 1992
PMID: 1522069
Genetic organization of the catabolic plasmid pJP4 from Ralstonia eutropha JMP134 (pJP4) reveals mechanisms of adaptation to chloroaromatic pollutants and evolution of specialized chloroaromatic degradation pathways.
Trefault N, De la Iglesia R, Molina AM, Manzano M, Ledger T, Perez-Pantoja D, Sanchez MA, Stuardo M, Gonzalez B., Environ. Microbiol. 6(7), 2004
PMID: 15186344

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