Identification of a tomatinase in the tomato-pathogenic actinomycete Clavibacter michiganensis subsp michiganensis NCPPB382

Kaup O, Grafen I, Zellermann EM, Eichenlaub R, Gartemann K-H (2005)
MOLECULAR PLANT-MICROBE INTERACTIONS 18(10): 1090-1098.

Journal Article | Published | English

No fulltext has been uploaded

Author
; ; ; ;
Abstract
The insertion site of a transposon mutant of Clavibacter michiganensis subsp. michiganensis NCPPB382 was cloned and found to be located in the gene tomA encoding a member of the glycosyl hydrolase family 10. The intact gene was obtained from a cosmid library of C. michiganensis subsp. michiganensis. The deduced protein TomA (543 amino acids, 58 kDa) contains a predicted signal peptide and two domains, the N-terminal catalytic domain and a C-terminal fibronectin III-like domain. The closest well-characterized relatives of TomA were tomatinases from fungi involved in the detoxification of the tomato saponin alpha-tomatine which acts as a growth inhibitor. Growth inhibition of C. michiganensis subsp. michiganensis by alpha-tomatine was stronger in the tomA mutants than in the wild type. Tomatinase activity assayed by deglycosylation of alpha-tomatine to tomatidine was demonstrated in concentrated culture supernatants of C. michiganensis subsp. michiganensis. No activity was found with the tomA mutants. However, neither the transposon mutant nor a second mutant constructed by gene disruption was affected in virulence on the tomato cv. Moneymaker.
Publishing Year
ISSN
PUB-ID

Cite this

Kaup O, Grafen I, Zellermann EM, Eichenlaub R, Gartemann K-H. Identification of a tomatinase in the tomato-pathogenic actinomycete Clavibacter michiganensis subsp michiganensis NCPPB382. MOLECULAR PLANT-MICROBE INTERACTIONS. 2005;18(10):1090-1098.
Kaup, O., Grafen, I., Zellermann, E. M., Eichenlaub, R., & Gartemann, K. - H. (2005). Identification of a tomatinase in the tomato-pathogenic actinomycete Clavibacter michiganensis subsp michiganensis NCPPB382. MOLECULAR PLANT-MICROBE INTERACTIONS, 18(10), 1090-1098.
Kaup, O., Grafen, I., Zellermann, E. M., Eichenlaub, R., and Gartemann, K. - H. (2005). Identification of a tomatinase in the tomato-pathogenic actinomycete Clavibacter michiganensis subsp michiganensis NCPPB382. MOLECULAR PLANT-MICROBE INTERACTIONS 18, 1090-1098.
Kaup, O., et al., 2005. Identification of a tomatinase in the tomato-pathogenic actinomycete Clavibacter michiganensis subsp michiganensis NCPPB382. MOLECULAR PLANT-MICROBE INTERACTIONS, 18(10), p 1090-1098.
O. Kaup, et al., “Identification of a tomatinase in the tomato-pathogenic actinomycete Clavibacter michiganensis subsp michiganensis NCPPB382”, MOLECULAR PLANT-MICROBE INTERACTIONS, vol. 18, 2005, pp. 1090-1098.
Kaup, O., Grafen, I., Zellermann, E.M., Eichenlaub, R., Gartemann, K.-H.: Identification of a tomatinase in the tomato-pathogenic actinomycete Clavibacter michiganensis subsp michiganensis NCPPB382. MOLECULAR PLANT-MICROBE INTERACTIONS. 18, 1090-1098 (2005).
Kaup, O, Grafen, I, Zellermann, EM, Eichenlaub, Rudolf, and Gartemann, Karl-Heinz. “Identification of a tomatinase in the tomato-pathogenic actinomycete Clavibacter michiganensis subsp michiganensis NCPPB382”. MOLECULAR PLANT-MICROBE INTERACTIONS 18.10 (2005): 1090-1098.
This data publication is cited in the following publications:
This publication cites the following data publications:

15 Citations in Europe PMC

Data provided by Europe PubMed Central.

Evaluation of a SUMO E2 Conjugating Enzyme Involved in Resistance to Clavibacter michiganensis Subsp. michiganensis in Solanum peruvianum, Through a Tomato Mottle Virus VIGS Assay.
Esparza-Araiza MJ, Banuelos-Hernandez B, Arguello-Astorga GR, Lara-Avila JP, Goodwin PH, Isordia-Jasso MI, Castillo-Collazo R, Rougon-Cardoso A, Alpuche-Solis AG., Front Plant Sci 6(), 2015
PMID: 26734014
Genome-wide transcriptome analysis of Clavibacter michiganensis subsp. michiganensis grown in xylem mimicking medium.
Hiery E, Adam S, Reid S, Hofmann J, Sonnewald S, Burkovski A., J. Biotechnol. 168(4), 2013
PMID: 24060828
Detoxification of α-tomatine by Cladosporium fulvum is required for full virulence on tomato.
Okmen B, Etalo DW, Joosten MH, Bouwmeester HJ, de Vos RC, Collemare J, de Wit PJ., New Phytol. 198(4), 2013
PMID: 23448507
Colonization and movement of GFP-labeled Clavibacter michiganensis subsp. michiganensis during tomato infection.
Chalupowicz L, Zellermann EM, Fluegel M, Dror O, Eichenlaub R, Gartemann KH, Savidor A, Sessa G, Iraki N, Barash I, Manulis-Sasson S., Phytopathology 102(1), 2012
PMID: 21879791
What does it take to be a plant pathogen: genomic insights from Streptomyces species.
Bignell DR, Huguet-Tapia JC, Joshi MV, Pettis GS, Loria R., Antonie Van Leeuwenhoek 98(2), 2010
PMID: 20396949
The Gram-positive side of plant-microbe interactions.
Francis I, Holsters M, Vereecke D., Environ. Microbiol. 12(1), 2010
PMID: 19624707
Genetic diversity of Streptomyces spp. causing common scab of potato in eastern Canada.
St-Onge R, Goyer C, Coffin R, Filion M., Syst. Appl. Microbiol. 31(6-8), 2008
PMID: 18947953
Streptomyces scabies 87-22 possesses a functional tomatinase.
Seipke RF, Loria R., J. Bacteriol. 190(23), 2008
PMID: 18835993
Virulence mechanisms of Gram-positive plant pathogenic bacteria.
Hogenhout SA, Loria R., Curr. Opin. Plant Biol. 11(4), 2008
PMID: 18639483
Tomatinase from Fusarium oxysporum f. sp. lycopersici is required for full virulence on tomato plants.
Pareja-Jaime Y, Roncero MI, Ruiz-Roldan MC., Mol. Plant Microbe Interact. 21(6), 2008
PMID: 18624637
Genome of the actinomycete plant pathogen Clavibacter michiganensis subsp. sepedonicus suggests recent niche adaptation.
Bentley SD, Corton C, Brown SE, Barron A, Clark L, Doggett J, Harris B, Ormond D, Quail MA, May G, Francis D, Knudson D, Parkhill J, Ishimaru CA., J. Bacteriol. 190(6), 2008
PMID: 18192393
The genome sequence of the tomato-pathogenic actinomycete Clavibacter michiganensis subsp. michiganensis NCPPB382 reveals a large island involved in pathogenicity.
Gartemann KH, Abt B, Bekel T, Burger A, Engemann J, Flugel M, Gaigalat L, Goesmann A, Grafen I, Kalinowski J, Kaup O, Kirchner O, Krause L, Linke B, McHardy A, Meyer F, Pohle S, Ruckert C, Schneiker S, Zellermann EM, Puhler A, Eichenlaub R, Kaiser O, Bartels D., J. Bacteriol. 190(6), 2008
PMID: 18192381
Virulence genes and the evolution of host specificity in plant-pathogenic fungi.
van der Does HC, Rep M., Mol. Plant Microbe Interact. 20(10), 2007
PMID: 17918619
Evolution of plant pathogenicity in Streptomyces.
Loria R, Kers J, Joshi M., Annu Rev Phytopathol 44(), 2006
PMID: 16719719

24 References

Data provided by Europe PubMed Central.

Detoxification of alpha-tomatine by Botrytis cinerea.
Quidde T, Osbourn AE, Tudzynski T., Physiol. Mol. Plant Pathol. 52(3), 1998
PMID: IND21807837
Two xylanase genes of the vascular wilt pathogen Fusarium oxysporum are differentially expressed during infection of tomato plants.
Ruiz-Roldan MC, Di Pietro A, Huertas-Gonzalez MD, Roncero MI., Mol. Gen. Genet. 261(3), 1999
PMID: 10323234
Gapped BLAST and PSI-BLAST: a new generation of protein database search programs.
Altschul SF, Madden TL, Schaffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ., Nucleic Acids Res. 25(17), 1997
PMID: 9254694
DNA sequencing with chain-terminating inhibitors.
Sanger F, Nicklen S, Coulson AR., Proc. Natl. Acad. Sci. U.S.A. 74(12), 1977
PMID: 271968
Tomatinase from Fusarium oxysporum f. sp. lycopersici defines a new class of saponinases.
Roldan-Arjona T, Perez-Espinosa A, Ruiz-Rubio M., Mol. Plant Microbe Interact. 12(10), 1999
PMID: 10517025
Effects of targeted replacement of the tomatinase gene on the interaction of Septoria lycopersici with tomato plants.
Martin-Hernandez AM, Dufresne M, Hugouvieux V, Melton R, Osbourn A., Mol. Plant Microbe Interact. 13(12), 2000
PMID: 11106022
A highly efficient transposon mutagenesis system for the tomato pathogen Clavibacter michiganensis subsp. michiganensis.
Kirchner O, Gartemann KH, Zellermann EM, Eichenlaub R, Burger A., Mol. Plant Microbe Interact. 14(11), 2001
PMID: 11763129
A large, mobile pathogenicity island confers plant pathogenicity on Streptomyces species.
Kers JA, Cameron KD, Joshi MV, Bukhalid RA, Morello JE, Wach MJ, Gibson DM, Loria R., Mol. Microbiol. 55(4), 2005
PMID: 15686551
Dehydrotomatine content in tomatoes.
Friedman M, Levin CE., J. Agric. Food Chem. 46(11), 1998
PMID: IND21976276
A saponin-detoxifying enzyme mediates suppression of plant defences.
Bouarab K, Melton R, Peart J, Baulcombe D, Osbourn A., Nature 418(6900), 2002
PMID: 12192413
Genomic analysis and initial characterization of the chitinolytic system of Microbulbifer degradans strain 2-40.
Howard MB, Ekborg NA, Taylor LE, Weiner RM, Hutcheson SW., J. Bacteriol. 185(11), 2003
PMID: 12754233
Dehydrotomatine and alpha-tomatine content in tomato fruits and vegetative plant tissues.
Kozukue N, Han JS, Lee KR, Friedman M., J. Agric. Food Chem. 52(7), 2004
PMID: 15053555

Export

0 Marked Publications

Open Data PUB

Web of Science

View record in Web of Science®

Sources

PMID: 16255248
PubMed | Europe PMC

Search this title in

Google Scholar